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Method for increasing oxygen production by blue algae endocytosing up-conversion nanoparticles
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A nanoparticle and oxygen production technology, applied in the field of medical materials to achieve the effect of increasing oxygen production
Pending Publication Date: 2020-05-01
TIANJIN UNIV
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However, the high-efficiency up-conversion process can only be achieved by doping trivalent rare earth ions because of its long metastable energy level lifetime.
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Embodiment 1
[0023] Comparison of oxygen production between cyanobacteria and cyanobacteria composites after upconverting nanoparticles co-cultured with cyanobacteria. Including the following steps:
[0025] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 2.
[0026] 3) Detect the oxygen production of cyanobacteria under near-infrared 808nm irradiation.
[0027] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
Embodiment 2
[0029] Oxygen production of cyanobacteria after co-cultivation of upconverting nanoparticles and cyanobacteria and tumor changes after intratumoral injection. Including the following steps:
[0031] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 4.
[0032] 3) Detection of oxygen production of cyanobacteria under near-infrared 808nm irradiation
[0033] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
Embodiment 3
[0035] Oxygen production of cyanobacteria after co-cultivation of upconverting nanoparticles and cyanobacteria and tumor changes after intratumoral injection. Including the following steps:
[0037] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 6.
[0038] 3) Detect the oxygen production of cyanobacteria under near-infrared 808nm irradiation.
[0039] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
[0040] Such as figure 1 Shown: the change of oxygen production of cyanobacteria after co-cultivation of upconversion nanomaterials and cyanobacteria;
[0041] Such as figure 2 Shown: TEM of upconverting nanoparticles.
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Abstract
The invention discloses a method for increasing oxygen production by blue algae endocytosing up-conversion nanoparticles. The method mainly comprises the following steps: 1) detecting a fluorescence spectrum of an up-conversion nanometer material; 2) co-culturing the up-conversion nanoparticles and the blue algae, and detecting the luminescence condition of the blue algae under a confocalmicroscope after the growth concentration of the blue algae reaches an OD value of 2-5; 3) detecting the oxygen production condition of the blue algae under the irradiation of near-infrared light with a wavelength of 808 nm; 4) detecting the oxygen production condition of the blue algae phagocytized into the up-conversion nanometer material under the irradiation of near infrared with a wavelength of 808 nm; and 5) comparing the blue algae oxygen production amounts detected twice. Through preparation of a compound of the up-conversion nanoparticles and the blue algae, the up-conversion nanoparticles can emit blue light with a wavelength of about 450 nm under irradiation of near-infrared light with a wavelength of 808 nm; after the nanoparticles and the blue algae are co-cultured, the blue algae phagocytize the up-conversion nanoparticles to enter the body; the blue light emitted by the nanoparticles can be used for photosynthesis; so oxygen is generated.
Description
technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a method for increasing oxygen production by cyanobacteria endocytosing upconverting nanoparticles. Background technique [0002] Cyanobacteria, also known as blue-green algae, is one of the ancient organisms on the earth. It contains chlorophyll a. It is an autotrophic organism that can perform photosynthesis. One of the biggest characteristics of algae that is different from eukaryotes is that it does not contain chloroplasts and only contains chlorophyll. The difference with photosynthetic bacteria is that photosynthetic bacteria (Rhodospirillum) perform relatively primitive photosynthetic phosphorylation, do not release oxygen during the reaction process, and are anaerobic organisms, while cyanobacteria can perform photosynthesis and release oxygen. There are also hypotheses that plants Chloroplasts are evolved from cyanobacteria. Because cyanobacteria...
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