Method for increasing oxygen production by blue algae endocytosing up-conversion nanoparticles
A nanoparticle and oxygen production technology, applied in the field of medical materials to achieve the effect of increasing oxygen production
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Embodiment 1
[0023] Comparison of oxygen production between cyanobacteria and cyanobacteria composites after upconverting nanoparticles co-cultured with cyanobacteria. Including the following steps:
[0024] 1) Detection of fluorescence spectra of upconversion nanomaterials.
[0025] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 2.
[0026] 3) Detect the oxygen production of cyanobacteria under near-infrared 808nm irradiation.
[0027] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
Embodiment 2
[0029] Oxygen production of cyanobacteria after co-cultivation of upconverting nanoparticles and cyanobacteria and tumor changes after intratumoral injection. Including the following steps:
[0030] 1) Detection of fluorescence spectra of upconversion nanomaterials.
[0031] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 4.
[0032] 3) Detection of oxygen production of cyanobacteria under near-infrared 808nm irradiation
[0033] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
Embodiment 3
[0035] Oxygen production of cyanobacteria after co-cultivation of upconverting nanoparticles and cyanobacteria and tumor changes after intratumoral injection. Including the following steps:
[0036] 1) Detection of fluorescence spectra of upconversion nanomaterials.
[0037] 2) Co-cultivate the upconverting nanoparticles and cyanobacteria, and detect the luminescence of the cyanobacteria after the OD value of the growth concentration of the cyanobacteria reaches 6.
[0038] 3) Detect the oxygen production of cyanobacteria under near-infrared 808nm irradiation.
[0039] 4) Detect the oxygen production of the cyanobacteria that have phagocytosed into the upconverting nanomaterial under near-infrared 808nm irradiation.
[0040] Such as figure 1 Shown: the change of oxygen production of cyanobacteria after co-cultivation of upconversion nanomaterials and cyanobacteria;
[0041] Such as figure 2 Shown: TEM of upconverting nanoparticles.
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