LAMP detection primer sets for wheat take-all gaeumannomyces graminis and method utilizing the same

A technology of wheat take-all pathogen and detection primers, which is applied in the field of genetic engineering, can solve the problems of easy confusion of early symptoms, and achieve the effect of avoiding damage and preventing confusion

Active Publication Date: 2020-05-01
HENAN UNIV OF SCI & TECH
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Problems solved by technology

In addition, wheat sheath blight and wheat root rot are also important soil-borne diseases of wheat, but the early symptoms of these three kinds of wheat soil-borne diseases are easy to be confused. To prevent and control in time at the initial stage to achieve twice the result with half the effort, it is necessary to develop a specific, sensitive, and rapid early diagnosis technology for wheat take-all

Method used

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  • LAMP detection primer sets for wheat take-all gaeumannomyces graminis and method utilizing the same
  • LAMP detection primer sets for wheat take-all gaeumannomyces graminis and method utilizing the same
  • LAMP detection primer sets for wheat take-all gaeumannomyces graminis and method utilizing the same

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Embodiment Construction

[0022] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention.

[0023] 1 Materials and methods

[0024] 1.1 Tested strains

[0025] A total of 14 kinds of plant pathogenic bacteria were tested, all of which were important pathogens of wheat and common soil-borne pathogens, including 8 strains of wheat take-all fungi and 1 strain of other pathogenic bacteria. The specific information is shown in Table 1.

[0026] surface Information on strains used for LAMP detection

[0027]

[0028] 1.2 Test medium

[0029] The test media were potato dextrose agar (PDA) and V8 juice agar (V8A).

[0030] PDA medium: 200 g potato, 20 g glucose, 15 g agar powder, 1 L distilled water.

[0031] V8A medium: V8 juice 200 mL, calcium carbonate 3 g, agar powder 15 g, distilled water 800 mL.

[0032] When cultivating strains in liquid, the medium used is the corresponding...

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Abstract

The invention relates to LAMP detection primer sets for wheat take-all gaeumannomyces graminis and a method utilizing the same, and belongs to the technical field of genetic engineering. The ITS of pathogen is taken as a target sequence so as to establish the LAMP detection technology of gaeumannomyces graminis, the adopted primers are strong in specificity, and a reaction system is high in sensitivity; and through the detection on diseased tissue, the reaction system can accurately and rapidly detect wheat take-all from diseased wheat tissue. The detection method is simple, rapid and sensitive without using expensive instruments, so that large-area promotion and use at grassroots plant protection stations can be achieved, and technical support can be provided for the early and accurate prevention of the wheat take-all.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a primer set and a method for detecting LAMP of the fungus Grassy graminosa LAMP. Background technique [0002] Henan Province is an important grain production base in the country and the most important main wheat producing area in my country. In recent years, with the implementation of conservation tillage techniques such as no-till sowing, shallow wheat sowing and straw returning, and mechanized cross-regional operations, the occurrence area of ​​wheat soil-borne diseases has continued to expand. In particular, wheat take-all disease has been spreading continuously since it was discovered in Henan Province in 1992. In recent years, it has shown a trend of large outbreaks in the whole province, seriously affecting the safe production of wheat in Henan Province. Wheat take-all disease is caused by the grass husk ( Gaeumannomyces graminis ) causes damage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6844C12Q1/04C12N15/11C12R1/645
CPCC12Q1/6895C12Q1/6844C12Q2531/119
Inventor 崔林开胡艳红和志华李梦琪邓俊丽
Owner HENAN UNIV OF SCI & TECH
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