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Lattice light sheet microscope and method for tiling lattice light sheet in lattice light sheet microscope

A microscope, lattice technology, applied in the field of lattice light sheet microscopy and tiling lattice light sheets in lattice light sheet microscopy, can solve the problems of very different optical structures and inability to easily use tiled LLS, etc. Achieve high-resolution, convenient, high-speed imaging capabilities

Active Publication Date: 2020-05-05
WESTLAKE UNIV
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  • Application Information

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Problems solved by technology

The optical configuration of this microscope is very different from that of the widely used LLSM, which prevents the tiled LLS technique from being easily implemented by most LLSM users

Method used

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  • Lattice light sheet microscope and method for tiling lattice light sheet in lattice light sheet microscope
  • Lattice light sheet microscope and method for tiling lattice light sheet in lattice light sheet microscope
  • Lattice light sheet microscope and method for tiling lattice light sheet in lattice light sheet microscope

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Embodiment Construction

[0041] In order to enable those skilled in the art to better understand the technical solutions of the present disclosure, the present disclosure will be described in detail below in conjunction with the accompanying drawings and specific embodiments. Embodiments of the present disclosure will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but are not intended to limit the present disclosure.

[0042] "First", "second" and similar words used in the present disclosure do not indicate any order, quantity or importance, but are only used for distinction. Words like "comprising" or "comprising" mean that the elements preceding the word cover the elements listed after the word, and do not exclude the possibility of also covering other elements.

[0043] FIG. 1( a ) shows a schematic optical configuration of an illumination path of an LLSM according to an embodiment of the present disclosure, which can directly adopt the ...

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Abstract

The present disclosure relates to a lattice light sheet microscope and a method for tiling a lattice light sheet in the lattice light sheet microscope. The lattice light sheet microscope comprises: asingle spatial light modulator (SLM) of which the optical modulation plane conjugates to an image plane of an excitation objective lens and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice; a transparent annular diaphragm, arranged at a plane conjugate to the entrance pupil of the excitation objective lens; and a first galvanometer, configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet. Hence, an LLS may be easily tiled without changing the hardware structure of a conventional LLSM, and the high-resolution and high-speed imaging capabilities of an LLSM are maintained on an imaging field of view that is much greater than the size of the light sheet, which is convenient, low cost and may be easily applied.

Description

technical field [0001] The present disclosure relates to a precision optical instrument and a method of using the same, and more particularly, to a lattice light sheet microscope and a method of tiling a lattice light sheet in the lattice light sheet microscope. Background technique [0002] Lattice light sheet microscopy (LLSM) uses a light sheet formed by various 2D optical lattices (Optical Lattice) to irradiate the sample for imaging. LLSM can image in two working modes: shaking mode, shaking mode, in which the optical lattice is scanned continuously to form a lattice light sheet, enabling high-speed imaging; and the super-resolution structured illumination microscopy (SR-SIM) mode, in which the optical lattice is discretely scanned to form a The imaging results with a resolution beyond the diffraction limit are obtained by means of the SR-SIM method. [0003] Compared with other commonly used light sheets in light sheet microscopy, lattice light sheet (LLS) has three m...

Claims

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Application Information

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IPC IPC(8): G02B21/00G02B21/06
CPCG02B21/002G02B21/06G02B21/0032G02B21/0076G02B21/367G01N21/6458G02B21/0048G02B21/14
Inventor 高亮陈壁彰
Owner WESTLAKE UNIV
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