Monoclonal neutralizing antibody against hpv33l1 and its application

A monoclonal antibody and protein technology, applied in the direction of antibodies, anti-tumor drugs, anti-viral agents, etc., can solve the problems of less research on monoclonal antibodies and limit the detection of HPV33 virus by monoclonal antibodies, and achieve the effect of improving the clinical negative conversion rate

Active Publication Date: 2022-05-31
中生方政生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The above data show that HPV33 is a common HPV type in cervical cancer patients, both abroad and domestically, but there are very few studies on its monoclonal antibody, which limits the use of monoclonal antibody to detect HPV33 virus and treat HPV33 virus

Method used

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  • Monoclonal neutralizing antibody against hpv33l1 and its application

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Embodiment 1

[0102] The No. 1 mouse with the highest antibody titer was used for fusion. Immunization with incomplete Freund's adjuvant 3 days before fusion

[0104]

[0108]

Embodiment 2

Embodiment 3

[0120] Antibodies were tested for their ability to neutralize activity by a pseudovirus-cell neutralization model.

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Abstract

The invention relates to the technical field of antibody medicine, in particular to a monoclonal neutralizing antibody of HPV33L1 and its application. The invention provides a specific HPV33 monoclonal neutralizing antibody and a hybridoma cell line producing the antibody, using one of the monoclonal neutralizing antibodies with the highest neutralizing activity titer, using indirect ELISA method, double antibody sandwich ELISA method, immune hybridization method, etc., can be widely used in the detection of HPV33 virus infection in clinical samples; use one of the monoclonal neutralizing antibodies with the highest neutralizing activity to prepare vaginal gel, vaginal washing liquid, external use Lotion, vaginal suppository, freeze-dried powder, vaginal effervescent tablet, etc. are used for the treatment of patients with HPV33 infection, which can effectively improve the clinical negative conversion rate of HPV33 patients. The invention has important significance for women's healthy development and public health prevention and control.

Description

Monoclonal neutralizing antibody against HPV33L1 and its application technical field [0001] The present invention relates to the technical field of antibody drugs, in particular to the monoclonal neutralizing antibody of HPV33L1 and its application. Background technique Human papillomavirus (Human Papillomavirus, referred to as HPV) is a spherical, double-stranded DNA virus. The particle diameter is 55-60 nm, the nucleocapsid is icosahedral symmetry, and consists of 72 pentamers of the major capsid protein L1 and minor capsid eggs. White L2 composition. It mainly invades human epithelial tissues and cells, and can cause the proliferation of squamous epithelium of human skin and mucous membranes, and then induce Various benign and malignant proliferative lesions. High-risk HPV infection is associated with the occurrence of a variety of malignant tumors, while low-risk HPV infection Anal and genital warts. [0003] High-risk HPV includes 16, 18, 26, 31, 33, 35, 39, 4...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/08C12N15/113C12N5/20A61K39/395A61P31/20A61P17/12A61P35/00
CPCC07K16/084A61P31/20A61P17/12A61P35/00A61K2039/505C07K2317/76C07K2317/565
Inventor 邹国宝魏颖颖郭光华蔺皓刘欣欣宋高尚沈江卫
Owner 中生方政生物技术股份有限公司
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