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A kind of biological nanocomposite material and its synthesis method and application

A bio-nano and composite material technology, applied in the field of bio-nano composite materials and their synthesis, can solve problems such as interactions that have not been clearly reported, and achieve the effects of good drug-carrying capacity, uniform size distribution, and simple operation.

Active Publication Date: 2022-07-26
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, Fe in relation to Parkinson's disease has not been clearly reported 3+ Interaction with α-synuclein

Method used

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  • A kind of biological nanocomposite material and its synthesis method and application
  • A kind of biological nanocomposite material and its synthesis method and application
  • A kind of biological nanocomposite material and its synthesis method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0141] Example 1: Synthesis of iron ion two-photon fluorescent probe

[0142] 1 mmol of tetrabutylammonium hydrogen sulfate, 22 mmol of 4-diethylaminobenzaldehyde and 10 mmol of 2-amino-4,6-dimethylpyrimidine were refluxed in 50 mL of 5M NaOH solution for 2 hours. The orange product was obtained by rotary evaporation and pure material was obtained by column chromatography (ethyl acetate:petroleum ether=1:1).

Embodiment 2

[0143] Example 2: Synthesis of α-synuclein two-photon fluorescent probe

[0144] 56 mmol of ruthenium trichloride, 112 mmol of phenanthroline and 3.7 mmol of lithium chloride were refluxed in 25 mL of DMF for 8 hours. Acetone was then added to the reaction mixture, which was cooled at 4°C overnight, the product Ru(phen) 2 Cl 2 Used without further purification. 12mmol Ru(phen) 2 Cl 2 and 10 mmol of dipyrido[3,2-A:2'.3'-C]phenazine in 1:1 aqueous methanol at reflux for 4 hours. The apparatus was cooled to room temperature, ammonium hexafluorophosphate was added to precipitate the product out of solution and the crude product was obtained by filtration. Purification by column chromatography (dichloromethane:acetonitrile=4:1) and recrystallization (ethanol:water=90:10) gave red-orange crystals.

Embodiment 3

[0145] Example 3: Synthesis of multifunctional bio-nanocomposites

[0146] In pH=7.4 PBS buffer solution, 20 μg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was added to 5 mL of 10 μg / mL copper sulfide nanospheres, stirred for 2 hours, and then 20 μg of N-hydroxysuccinimide was added and stirred for half an hour, and then 100 μL of the 50 μg / mL iron ion two-photon fluorescent probe prepared in Example 1 of the present invention was added to the mixture overnight with stirring. The product was collected by centrifugation and purified by washing several times with deionized water, then resuspended in 5 mL of PBS buffer pH=7.4. Finally, 100 μL of 50 μg / mL α-synuclein two-photon fluorescent probe prepared in Example 2 of the present invention was added to the solution and stirred overnight at room temperature. The resulting product was collected by centrifugation, purified by several washes with deionized water, and then resuspended in 5 mL of PBS buffer pH=7.4....

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Abstract

The invention discloses a multifunctional biological nanocomposite material and a synthesis method thereof. The nanocomposite material is composed of iron ion two-photon fluorescent probe, α-synuclein two-photon fluorescent probe, porous hollow copper sulfide nanoparticle, DNA Nanogate and α-synuclein aggregate therapeutic drug complexed. The two two-photon fluorescent probes disclosed in the invention have short synthesis steps, simple operation, high selectivity and high sensitivity; copper sulfide nanoparticles are synthesized by a one-pot method, have uniform size distribution, large specific surface area and good drug loading ability. The invention also discloses the application of the multifunctional biological nanocomposite material in the two-photon fluorescence lifetime detection and imaging of iron ions and α-synuclein aggregates in vitro and in vivo, and analyzes the iron ions related to Parkinson's disease for the first time. The interaction between ions and α-synuclein, and the construction of an integrated platform for the diagnosis and treatment of chemotherapy and photothermal therapy is of great significance for the study of the treatment and diagnosis of Parkinson's disease.

Description

technical field [0001] The invention belongs to the technical field of nanometer material analysis and detection, and relates to a biological nanocomposite material and a synthesis method and application thereof. Background technique [0002] Parkinson's disease is a common neurodegenerative disease, first proposed by James Parkinson in 1817, and is the second most common neurodegenerative disease in humans after Alzheimer's disease. The etiology and pathogenesis are not yet clear. The main pathological factors include degeneration and death of midbrain substantia nigra dopamine neurons, significant reduction of striatum, and Lewy bodies in the cytoplasm of substantia nigra neurons. Alpha-synuclein is the main component of Lewy bodies, which are located at the presynaptic terminal of neurons, and is a protein composed of 140 amino acids. Its mutation, aggregation and excessive accumulation are associated with a series of diseases including Parkinson's disease. Neurodegenera...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/06C07F15/00C07D239/42G01N21/64A61K41/00A61K49/00A61P25/16A61P25/28
CPCC09K11/06C07D239/42C07F15/0046G01N21/6428A61K49/0021A61K49/0093A61K41/0052A61K41/0042A61P25/16A61P25/28G01N2021/6432C09K2211/1044C09K2211/1007C09K2211/185A61K2300/00
Inventor 田阳陈伟闻郑婷婷
Owner EAST CHINA NORMAL UNIV
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