32results about How to "Enhanced two-photon absorption cross section" patented technology

The invention belongs to the field of two-photon polymerization, and particularly relates to the application of coumarin and / or benzylidene cycloalkanone dyes in the preparation of two-photon polymerization resins. The present invention is a straight-chain or multi-branched coumarin two-photon dye, a straight-chain or multi-branched benzylidene cycloalkanone two-photon dye, or a functional group containing both coumarin and benzylidene cycloalkane ketone The two-photon dye is used as a one-component initiator to prepare two-photon polymerization resin with low initiation threshold, low small molecule residue and high resolution.

The invention discloses a pyrrolidine double-fluorine-boron strong fluorescent dye and a preparation method and application thereof, wherein the structure is as shown in formula (1), R1, R2 and R3 areH or C1-C6 linear or branched alkyl group, R5 is H, Cl, xylylene group, nonane group, benzene ring group, OR9, NR9R10, or SR9. R4, R6, R7 and R8 are each independently H, C1-C6 linear or branched alkyl, C1-C6 linear or branched cycloalkane group; wherein R9 and R10 are each independently H, CH2COOEt, oxo group, xylyl group, C1-C6 linear or branched alkyl or C1-6 linear or branched cycloalkane group; the pyrrolidine double-fluorine-boron strong fluorescent dye has the advantages of up to 998 GM double-photon absorption cross section at 700-900nm, high molar absorption coefficient, high fluorescence quantum yield, high light stability and double photon fluorescence and the like.

The invention disclose a two photon absorption material capable of imaging alive cells, which is a terpyridine derivative Zn(II)S2 complex shown by the following structural formula. The complex is a two photon absorption material having a property of two-photon fluorescence imaging of alive tissue cells, has the characteristics of large absorption area, low excitation energy, large wavelength, high penetrability, less light injuries, non-toxicity and the like and is suitable to be used for detecting alive cells.

The invention belongs to the field of organic nonlinear optical materials, and particularly relates to a triphenylamine two-photon fluorescence probe compound and a preparation method and an application thereof. The preparation method of the triphenylamine two-photon fluorescence probe compound comprises the following steps of: injecting anhydrous benzene into 4-nitrapyrin-2, 6-dimethyl isophthalate and triphenylphosphine under the protection of nitrogen; increasing temperature, refluxing, and cooling; filtering and washing to obtain chlorination-4-methylene triphenylphosphine group-pyridine-2,6-dimethyl isophthalate, and dissolving into methanol; mixing and stirring tri(4-formylphenyl)amine and absolute methanol; adding the methanol solution of the chlorination-4-methylene triphenylphosphine group-pyridine-2,6-dimethyl isophthalate and the methanol solution of sodium methylate; stirring at 0-5 DEG C for 1-2 hours, stirring at normal temperature for 3-4 hours; separating through column chromatography. The triphenylamine two-photon fluorescence probe compound disclosed by the invention has the advantages of outstandingly enhanced two-photon absorption section, good water solubility, higher reaction activity and very high coordination capacity and can be applied to silver ion detection and pH detection.

The invention discloses a D-pi-A-pi-D' compound as well as synthesis and application thereof. The structure of the D-pi-A-pi-D' compound is shown as formula I or formula II. The synthesis method of the D-pi-A-pi-D' compound comprises the following steps: step 1, unilateral Horner-Wadsworth-Emmons reaction is performed on 4-(9H-carbazol-9-yl)benzaldehyde or 3,4-dimethoxybenzaldehyde and a compoundshown as formula V to obtain a compound shown as formula III or formula IV; step 2, the Horner-Wadsworth-Emmons reaction is performed on 9-ethyl-9H-carbazole-3-carboxaldehyde and the compound shown asformula III or formula IV to obtain the D-pi-A-pi-D' compound shown as formula I or formula II. According to the invention, the D-pi-A-pi-D' compound has the advantages of large two-photon absorptioncross section, high fluorescence quantum yield and long fluorescence lifetime, so that the compound can be used as a high-performance two-photon absorption and two-photon fluorescence material. (Theformulas are shown in the description).

The present invention relates to the technical field of two-photon fluorescent probes, in particular, to the use of a two-photon fluorescent probe, which is used in cell and tissue imaging to detect lipid raft content and distribution dynamics. The probe can improve the accuracy, precision, sensitivity, and imaging resolution of lipid raft detection, and provide new ideas for further prevention and control of neurodegenerative diseases such as Alzheimer's disease and prion disease.

The invention relates to two photon polymerization initiating system, especially relates to a high- effective method for synthesizing coumarins dyestuff connected with stilbene and its application in two photon polymerization. It comprises following steps: dissolving the mediate coumarins dyestuff with A1 gene into organic solvent; adding alkaline catalyst solution into it; dropping the organic phosphonic solvent carrying A2 gene with the same molar weight to that of coumarins dyestuff into reation system, heating to solvent boiling and refluxing; cooling and separting organic phase from water phase through extraction with organic solvent; drying and evaporating organic phase, separating coarse product with column chromatography, heating with iodine with its amount the same to that of catalyst, getting coumarins dyestuff connected with stilbene, which is used as photosensitiser in photon polymerization, and its structure is as follows: the substituents of A1 and A2 are the same or different, and are chose from alkyl, alkoxy, amido, heterocycle or substituted phenyl.

The invention discloses a multifunctional biological nanocomposite material and a synthesis method thereof. The nanocomposite material is composed of iron ion two-photon fluorescent probe, α-synuclein two-photon fluorescent probe, porous hollow copper sulfide nanoparticle, DNA Nanogate and α-synuclein aggregate therapeutic drug complexed. The two two-photon fluorescent probes disclosed in the invention have short synthesis steps, simple operation, high selectivity and high sensitivity; copper sulfide nanoparticles are synthesized by a one-pot method, have uniform size distribution, large specific surface area and good drug loading ability. The invention also discloses the application of the multifunctional biological nanocomposite material in the two-photon fluorescence lifetime detection and imaging of iron ions and α-synuclein aggregates in vitro and in vivo, and analyzes the iron ions related to Parkinson's disease for the first time. The interaction between ions and α-synuclein, and the construction of an integrated platform for the diagnosis and treatment of chemotherapy and photothermal therapy is of great significance for the study of the treatment and diagnosis of Parkinson's disease.

The invention belongs to the technical field of fine chemical engineering, and particularly relates to a preparation method of a two-photon fluorescence pH probe and application in cell imaging thereof. The probe is prepared by taking dicyan-biphenyl-ethylene as a matrix, and comprises carbazolyl, di-cyano and n-octyl. Because the molecular structure of the probe has a longer flexible chain, the lipophicity is stronger, the probe can penetrate a cell wall (or cell membrane) into a cell to stain the cell for detecting the pH in the cell; the molecular structure of the probe belongs to D-pi-A type molecules, the two-photon absorption cross section (delta) is improved, so that the probe has higher imaging definition and resolution; in addition, the probe has a large Stokes shift in the marking process, thereby greatly improving the imaging accuracy and precision; the fluorescence quantum yield (phi) of the probe in a solvent is higher, the two-photon emission cross section is larger, so that the higher the brightness of the imaging is, the clearer the image is, and the higher the resolution is.

The invention relates to a stellated compound which contains 2, 4, 6-tri [2-thienyl)-1, 3, 5-sym-triazine units, wherein the structural formula of the stellated compound is as shown in a figure. The preparation method for the stellated compound is as follows: 2, 4, 6-tri [2-(5- bromine thienyl)]-1,3,5-sym-triazine prepared by bromination reaction of the 2, 4, 6-tri [2- thienyl)-1, 3, 5-sym-triazine undergoes Suzuki reaction with mono-bromination arene ArBr under the catalysis of Ed(PPh3)4, so as to obtain the stellated compound of the 2, 4, 6-tri (2- thienyl)-1, 3, 5-sym-triazine units; or the 2,4,6-tri [2-(5- bromine thienyl)]-1,3,5-sym-triazine and mono-acetylenyl-substituted aromatic ArH undergo Sonogashira reaction under the catalysis of the Pd(PPh3)4 and CuI, so as to obtain the stellated compound which contains the 2, 4, 6-tri (2- thienyl)-1, 3, 5-sym-triazine units. The stellated compound which contains the 2, 4, 6-tri (2- thienyl)-1, 3, 5-sym-triazine units is applied to the field of organic luminescent materials or organic two-photon absorption materials.

The invention discloses an organic-inorganic nanocomposite two-photon fluorescent material as well as a preparation method and an application thereof. The organic-inorganic nanocomposite two-photon fluorescent material is formed by compositing an organic component and an inorganic component under interfacial coordination of Zn(NCS)2, wherein the organic component is ligand L, namely, 4-([2,2':6',2''-terpyridine]-4'-yl)-N,N-diphenylamine, and the inorganic component is ZnS. The ligand L and ZnS nanoparticles are composited under the interfacial coordination of Zn(NCS)2. The L-ZnS nanocompositematerial has the characteristics of large two-photon absorption cross section (capable of reaching 2992.08 GM), low excitation energy, high penetrability, small optical damage, low toxicity and the like and realizes long-wavelength emission, so that damage to cells is avoided, and the material can be used for detecting copper ions in living cells and has obvious application value.

The invention discloses a nitrogen-doped graphene quantum dot, a preparation method thereof and application thereof as a two-photon fluorescence probe to imaging of living cells. The nitrogen-doped graphene quantum dot is simple in preparation method, is good in imaging effect when applied to two-photon fluorescence imaging of living cells, and has extremely strong two-photon fluorescence in a relatively wide pH scope, and the two-photon absorption cross section can reach 48000 GM; the nitrogen-doped graphene quantum dot is extremely easy to dissolve in water, free of toxicity and good in light stability; and a solution of the nitrogen-doped graphene quantum dot has extremely strong stability, is capable of keeping stable within 24 months, and has wide application prospect.

The invention discloses a nitrogen-sulfur co-doped graphene quantum dot material, its preparation method and its application. The method of the invention adopts a bottom-up preparation method from small organic molecules 1,3,6-trinitropyrene , One-step synthesis of nitrogen-sulfur co-doped graphene quantum dots in alkaline aqueous solution of ammonium sulfite. The method of the invention has simple steps, high repeatability and high output. Since the surface of the prepared nitrogen-sulfur co-doped graphene quantum dots contains more functional groups of hydroxyl, amino and sulfonic acid groups, it is very soluble in water and exists stably in aqueous solution for a long time. The two-photon fluorescence performance of the graphene quantum dots of the present invention is significantly improved, and the imaging effect and the imaging depth in biological tissues are further improved. The nitrogen-sulfur co-doped graphene quantum dots prepared by the method of the present invention are used as two-photon fluorescent probes, which have It has good two-photon fluorescence characteristics, can emit strong fluorescence under near-infrared wavelength excitation, and can perform good imaging effects on biological tissues.