Preparation and application of a nitrogen-doped graphene quantum dot two-photon fluorescent probe

A technology of graphene quantum dots and nitrogen doping, which is applied in the field of preparation of two-photon fluorescent probes, and achieves the effect of simple preparation method
CN104109534BInactive Publication Date: 2015-11-25THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a nitrogen-doped graphene quantum dot, a preparation method thereof and application thereof as a two-photon fluorescence probe to imaging of living cells. The nitrogen-doped graphene quantum dot is simple in preparation method, is good in imaging effect when applied to two-photon fluorescence imaging of living cells, and has extremely strong two-photon fluorescence in a relatively wide pH scope, and the two-photon absorption cross section can reach 48000 GM; the nitrogen-doped graphene quantum dot is extremely easy to dissolve in water, free of toxicity and good in light stability; and a solution of the nitrogen-doped graphene quantum dot has extremely strong stability, is capable of keeping stable within 24 months, and has wide application prospect.
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Description

technical field

[0001] The invention relates to a preparation method of a two-photon fluorescent probe and its application, in particular to a preparation method of a nitrogen-doped graphene quantum dot two-photon fluorescent probe and its application in live cell imaging. Background technique

[0002] Two-photon fluorescence microscopy has the characteristics of near-infrared excitation, deep penetration of excitation light into biological tissues, low phototoxicity, and weak background fluorescence of organisms, and is significantly superior to traditional single-photon fluorescence microscopy. , is one of the most important and powerful tools in the field of life science research. The emergence of laser scanning confocal two-photon fluorescence microscopy has promoted the development of two-photon fluorescence microscopy imaging technology, which has shown excellent application prospects in the field of living biological tissue imaging, and has attracted more and more att...

Claims

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