Automatic culture box for living cells

Abstract

The invention provides an automatic culture box for living cells and is used for suspension culture of cells. A U-shaped support and a culture flask are arranged in a culture cavity, the culture flaskis mounted on the U-shaped support, a swing mechanism is arranged in the U-shaped support to swing the culture flask back and forth, meanwhile, a reciprocating mechanism is also arranged in the culture cavity and can drive the U-shaped support to reciprocate bilaterally, under the cooperation of the swing mechanism and the reciprocating mechanism, a culture solution in the culture flask can be stirred continuously, the adherence phenomenon of cells can be prevented, meanwhile, as stirring equipment is not required to be arranged in the culture flask, excessive cell death can be prevented, andthe survival rate of the cells can be increased.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an automatic incubator for living cells. Background technique [0002] Cell culture refers to a method of simulating the in vivo environment in vitro to enable cells to survive, grow, reproduce and maintain the main structure and function. Cell culture generally includes adherent culture, suspension culture and immobilized culture, among which suspension culture refers to It is a tissue culture system that cultures single cells and small cell clusters in a liquid medium that is constantly stirred or shaken. It is a culture method for non-anchorage-dependent cells. [0003] Most of the existing suspension culture equipment is to directly extend the agitation mechanism into the culture medium for agitation. The agitation equipment will generate shear force and heat during agitation. The shear force will cause cell death, and the heat will affect the growth of the culture mediu...

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Problems solved by technology

[0003] Most of the existing suspension culture equipment is to directly extend the agitation mechanism into the culture medium for agitation. The agitation equipment will generate shear force and heat during agitation. The shear force will cause cell death, and the heat will affect the growth of the culture medium. Temperature, which in turn affects the culture of cells, causing a lot of loss

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