Extraction method for polyphenol compounds from ginseng leaves

A polyphenolic compound and extraction method technology, applied in the field of extraction of polyphenolic compounds in ginseng leaves, can solve the problems of few polyphenolic compound extraction studies, long reaction period, low extraction efficiency, etc., and achieve accurate process parameters. Reliable, low cost of industrialization, and the effect of improving the dissolution rate

Inactive Publication Date: 2020-05-22
JILIN UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0004] Traditional plant polyphenol extraction methods mainly include ultrasonic extraction, microwave extraction, supercritical fluid extraction, and fl

Method used

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  • Extraction method for polyphenol compounds from ginseng leaves

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specific Embodiment approach

[0041] In order to clearly and completely describe the technical solution of the present invention and its specific working process, in conjunction with the accompanying drawings, the specific implementation of the present invention is as follows:

[0042] The present invention adopts the combination of low-temperature plasma treatment and compound enzyme extraction to extract polyphenolic compounds in ginseng leaves in stages, and takes the total polyphenol yield as an evaluation index to determine the process parameters of the low-temperature plasma treatment stage and the compound enzyme extraction stage . The specific process route is as follows:

[0043] Raw material processing→low temperature plasma treatment→compound enzyme extraction→centrifugation→ginseng leaf polyphenol compound sample solution→decompression concentration→lyophilization→storage.

Embodiment 1

[0046] a) Raw material handling:

[0047] Washing, drying, pulverizing and passing through a 60-mesh sieve in sequence to prepare ginseng leaf powder, and storing it in a dry environment for later use;

[0048] b) Low temperature plasma treatment:

[0049] Weighing 0.5 g of processed ginseng leaf powder in step a) and putting it into a low-temperature plasma treatment apparatus to carry out low-temperature plasma treatment modification;

[0050] The conditions for low-temperature plasma treatment are: the vacuum degree is less than 100Pa, and the stable range of vacuum degree is 70-90Pa; the discharge power is 200W, and the low-temperature plasma treatment time is 30s;

[0051] c) Complex enzyme extraction:

[0052] The ginseng leaf powder after step b) low-temperature plasma treatment is added in the compound enzyme extracting solution by the liquid material ratio of 80ml / g to carry out compound enzyme extraction, then carry out 15min centrifugal treatment under the conditi...

Embodiment 2

[0062] a) Raw material handling:

[0063] Washing, drying, pulverizing and passing through a 60-mesh sieve in sequence to prepare ginseng leaf powder, and storing it in a dry environment for later use;

[0064] b) Low temperature plasma treatment:

[0065] Weighing 0.5 g of processed ginseng leaf powder in step a) and putting it into a low-temperature plasma treatment apparatus to carry out low-temperature plasma treatment modification;

[0066] The conditions for low-temperature plasma treatment are: the vacuum degree is less than 100Pa, and the stable range of vacuum degree is 70-90Pa; the discharge power is 300W, and the low-temperature plasma treatment time is 30s;

[0067] c) Complex enzyme extraction:

[0068] The ginseng leaf powder after step b) low-temperature plasma treatment is added in the compound enzyme extracting solution by the liquid material ratio of 80ml / g to carry out compound enzyme extraction, then carry out 15min centrifugal treatment under the conditi...

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Abstract

The invention relates to a natural product extraction technology, and specifically provides an extraction method for polyphenol compounds from ginseng leaves. According to the method, the ginseng leaves are used as raw materials, and low-temperature plasma-complex enzyme is used to extract the polyphenol compounds in the ginseng leaves. The extraction method is divided into the following two stages: low-temperature plasma treatment is used in the early stage, and complex enzyme extraction is used in the later stage. The method provides a novel technology for extracting the polyphenol compoundsfrom the ginseng leaves, the raw materials are rich in source, the extraction conditions are accurate and reliable, the extraction efficiency is high, the equipment is simple to operate, the production cost of the polyphenol compounds is reduced, and the industrial production of the polyphenol compounds is facilitated.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting polyphenolic compounds in ginseng leaves. Background technique [0002] Ginseng leaves are the dried leaves of the perennial herb Araliaceae ginseng. It is one of the medicinal and edible raw materials with health care effects in my country. It is often used to treat qi deficiency cough, heat and irritability, thirsty wounds, unclear head, tired limbs, etc. disease. Modern pharmacological studies have shown that it has a good therapeutic effect on tumors, hepatitis, coronary heart disease, and Addison's disease. It can strengthen the excitation process of the cerebral cortex, accelerate the conduction of nerve impulses, shorten the latent period of nerve reflexes, enhance conditioned reflexes, and improve Skills of analyze. In September 2012, after the state approved artificial planting of ginseng as a new resource food, the...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K127/00
CPCA61K36/258A61K2236/17A61K2236/19A61K2236/30
Inventor 赵玉娟高峰徐艳阳吴文福陈艳颜伟强姜雯雯
Owner JILIN UNIV
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