Preparation process of four ingredients in herba cistanche and application of four ingredients

A technology of Cistanche deserticola and Cistanche deserticola water extract, which is applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives and other directions, can solve the problems of unclear active ingredients, lack of microscopic analysis of medicinal effect substances, etc., so as to reduce costs, pollution, The effect of strong repeatability and stable process quality

Pending Publication Date: 2020-05-22
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, although Cistanche deserticola is used for neuroprotection in the prior art to achieve better curative effect, the active ingredients are not yet clear, and there is a lack of microscopic analysis of the effective substances.

Method used

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  • Preparation process of four ingredients in herba cistanche and application of four ingredients
  • Preparation process of four ingredients in herba cistanche and application of four ingredients
  • Preparation process of four ingredients in herba cistanche and application of four ingredients

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Example 1 Cistanche Extraction Process Investigation

[0089] Soaking time, extraction method, extraction solvent, extraction time, extraction times, solid-liquid ratio and extraction particle size are optimized and investigated by single factor investigation method, and the medicinal material of Cistanche deserticola is cut into less than 1cm 3The small pieces were soaked in water for 1 hour, decocted and extracted, and the number of extractions was 2 times; the investigation items of the orthogonal design experiment were the extraction solvent, extraction time and solid-liquid ratio, and then the response surface design was used to analyze the extraction solvent, extraction time and solid-liquid ratio. The final investigation result is: water is used as the extraction solvent, the extraction time is 1h, and the ratio of solid to liquid is 1:11.85. The investigation is based on the yield of the extract, and the content determination method is based on the chromatograph...

Embodiment 2

[0104] Macroporous resin purification process investigation of embodiment 2 cistanche extract

[0105] The alcohol precipitation method is used to remove the polysaccharides in the water extract of Cistanche deserticola, and the alcohol precipitation process is optimized from the concentration and density of ethanol. Precipitating the solution, using ethanol with a concentration of 95% to remove the polysaccharides in the Cistanche cistanche water extract obtained in step 1), so that the final concentration of the alcohol precipitating solution is 50-70%, obtaining the supernatant of the alcohol precipitating, and the alcohol precipitating on the The supernatant was allowed to stand at 4°C for 12 hours, and the supernatant was collected by centrifugation. The ethanol was recovered under reduced pressure, and the aqueous solution was lyophilized to obtain the solid matter of the alcohol precipitation supernatant.

[0106] The optimization index is the transfer rate of echinacos...

Embodiment 3

[0115] Example 3 Preparation of four high-purity chemical components using the HPLC-DAD / MS component automatic collection system

[0116] Take Cistanche medicinal material (particle size is less than 1cm 2 ) 250g, placed in a 10L round bottom flask, added 11.85 times the amount of water (2750mL), soaked for 1h, extracted twice, heated and decocted for 1h each time, filtered, and combined the filtrates. Rotary evaporation, concentrate to 357mL (0.7g crude drug / mL), add 773mL 95% ethanol, stand at 4°C for 12h, centrifuge to take the supernatant, rotary evaporation, recover under reduced pressure to remove ethanol, freeze-dry the water to obtain the supernatant of alcohol precipitation Liquid solids 130g.

[0117] Get the alcohol-precipitated supernatant solid after alcohol precipitation, dissolve in water, make the homogeneous clear solution that echinacoside concentration is 2.8mg / mL, take the processed 32g HPD-300 and 32g NKA-9 (wet weight ) Wet packing, put into a glass col...

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Abstract

The invention specifically discloses a method for extracting, purifying and simultaneously preparing four high-purity ingredients of echinacoside, verbascoside, isoacteoside and tubuloside from herbacistanche and an application of the four single ingredients and compositions of the four single ingredients in different proportions in neuroprotection and bone protection. The method comprises stepsas follows: extraction of medicinal materials, alcohol precipitation with an extracting solution, macroporous resin purification and refining in combination with an HPLC-DAD/MS component automatic collecting system, that is, the medicinal materials of herba cistanche are extracted with a hot reflux method; preliminary purification is performed in an alcohol precipitation manner; the four ingredients are further purified with a macroporous resin purification method, and phenylethyl alcohol glycoside parts (with the content larger than or equal to 50% and meeting the requirements of five traditional Chinese medicine parts of novel medicines) of the required four ingredients are obtained; and finally, four ingredient monomers are efficiently prepared in a high-purity manner by use of the HPLC-DAD/MS component automatic collecting system and have the purity higher than 98%; and besides, the neuroprotection and bone protection functions of the four single ingredients and compositions of thefour single ingredients in different proportions is studied.

Description

technical field [0001] The invention belongs to the field of natural products and relates to the medical application of natural products, in particular to the application of four component monomers in Cistanche deserticola and their compositions in different proportions in neuroprotection and bone protection. Background technique [0002] Cistanche deserticola (Cistanche deserticola Ma) is a plant of the genus Cistanche in the family Cistanche. It is parasitic on the roots of plants of the genus Salix, such as Haloxylon, red willow, white thorn, sycamore, and Artemisia. The medicinal part of Cistanche deserticola is a dry fleshy stem with scales. It is currently recorded that Cistanche is divided into four varieties, namely, Cistanche, Cistanche, Cistanche, Salt Cistanche and Salt Cistanche. The varieties accepted in the Pharmacopoeia are Cistanche and Cistanche. Cistanche has been recorded in the ancient medical classics "Shen Nong's Materia Medica", which has the function...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H15/18A61P25/28A61P19/08
CPCC07H1/08C07H15/18A61P25/28A61P19/08
Inventor 李飞方佳艺李英祺吴闪闪李萍
Owner CHINA PHARM UNIV
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