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Application of hsp90ab1 protein in identification of Streptococcus suis meningitis

A technology for identifying swine and streptococci, which is applied in the field of swine disease identification, can solve the problems that there are no auxiliary diagnostic indicators for meningitis, and achieve the effect of simple and easy detection

Active Publication Date: 2021-11-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the retrieval, in the studies so far, there is no report on using it as an auxiliary diagnostic index for meningitis

Method used

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  • Application of hsp90ab1 protein in identification of Streptococcus suis meningitis
  • Application of hsp90ab1 protein in identification of Streptococcus suis meningitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Method

[0015] 1. The source of the strain and the establishment of the infection model of Streptococcus suis type 2

[0016] Source of strains: All SS2 strains used were isolated from SS2-infected pigs. JZLQ022 strain was isolated from the brain tissue of pigs with meningitis; JZLQ001 strain was isolated from the mandibular lymph nodes of pigs with arthritis.

[0017] Experimental grouping: 18 26-day-old healthy Bama miniature pigs were randomly divided into three groups, 6 in each group, which were meningitis group, non-meningitis group and healthy control group.

[0018] Model preparation: Inject 200 μL of pig ear vein containing 5 × 10 6 PBS of CFUs JZLQ022; 200 μL of pig ear vein injection containing 5 × 10 6 CFUs JZLQ001 in PBS; pigs in the healthy control group were injected with 200 μL PBS intravenously.

[0019] After infection, the clinical symptoms of pigs in each group were monitored, including appetite, mental state, and body temperature.

[0020...

Embodiment 2

[0038] 1. Method

[0039] 1. Fabrication of antibody-coated microtiter plates

[0040] ① Take rabbit anti-pig HSP90AB1 antibody, detect antibody concentration by BCA, use coating buffer (1.59g / LNa 2 CO 3 , 2.93g / L NaHCO 3 , 1L ddH 2 O, PH=9.4) Adjust the antibody concentration to 7 μg / mL to obtain the antibody coating solution;

[0041] ② Add 100 μL / well of the antibody coating solution to a blank ELISA plate and incubate overnight at 4°C.

[0042] 2. Detection of HSP90AB1 protein level in porcine serum samples by ELISA

[0043] ① Take the overnight coated ELISA plate, discard the coating solution, add washing buffer (8.0g / L NaCl, 0.2g / L KCl, 3.58g / L NaCl, 200μL / well 2 HPO 4 , 0.27g / L KH 2 PO 4 , 500μL / L Tween-20, 1L ddH2O), shake and wash 3 times, 5min each time, and pat dry;

[0044] ② Add 150 μL / well of blocking solution (prepared with 0.05 g / mL skim milk), and block at 37°C for 1 hour;

[0045] ③ Discard the blocking solution and wash (the method is the same as ...

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Abstract

The invention discloses the application of HSP90AB1 protein in identifying Streptococcus suis meningitis, and provides a new application of HSP90AB1 protein, which is to detect HSP90AB1 protein as a target, and carry out auxiliary diagnosis of SS2 meningitis according to the result; new and traditional Compared with the method, the present invention is more accurate and sensitive, and the protein does not show high expression in non-meningitis infection or healthy pig serum, so not only can SS2 infection be confirmed, but at the same time, it can be detected when there are no clinical symptoms of meningitis Auxiliary diagnosis of meningitis facilitates targeted treatment in a timely manner; the detection of protein expression level in the present invention is very simple, and accurate results can be obtained in a short time. It is also relatively easy to produce corresponding commercial reagents and kits for protein detection, and can be more widely used in the detection of sick pigs.

Description

technical field [0001] The invention discloses an application of HSP90AB1 protein in identification of Streptococcus suis meningitis. The identification of Streptococcus suis meningitis is carried out through protein changes in blood; it belongs to the technical field of pig disease identification methods. Background technique [0002] Streptococcus suis type 2 (SS2) is an important zoonotic pathogen that can cause severe clinical symptoms such as meningitis, arthritis, endocarditis and sepsis in humans or pigs. It not only brings huge economic losses to the pig industry all over the world, but also seriously threatens public health and safety. Compared with non-meningitis-type SS2 infection, meningitis caused by meningitis-type SS2 infection is the most serious damage to the body, not only leading to cerebral edema, infiltration of inflammatory cells in the brain, neurological symptoms, and even death of animals, even if cured , often accompanied by serious sequelae such a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/535G01N33/558
CPCG01N33/535G01N33/558
Inventor 雷连成刘洪涛吴桐姜合祥贾丽孙莹莹孙长江
Owner JILIN UNIV
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