Kit for quantitatively detecting cTnT/NT-proBNP/D-dimer and application thereof

Abstract

The embodiment of the invention belongs to the technical field of fluorescence immunochromatography, and relates to a kit for quantitatively detecting cTnT / NT-proBNP / D-dimer and application thereof, the kit comprises a detection card, the detection card comprises a card shell and a test paper card, the test paper card comprises a PVC bottom plate, and the PVC bottom plate is provided with a sample pad, a conjugate pad, a nitrocellulose membrane and absorbent paper; three monoclonal antibodies, namely anti-cardiac troponin T, anti-N-terminal atrial natriuretic peptide and anti-D dimer, which are respectively marked by rare earth Eu < 3 + > fluorescent microspheres, and a goat anti-chicken IgY antibody marked by rare earth Eu < 3 + > fluorescent microspheres are adsorbed on the combination pad; the nitrocellulose membrane is provided with a first detection line, a second detection line, a third detection line and a quality control line, the first detection line is coated with a D-dimer monoclonal antibody, the second detection line is coated with a cTnT monoclonal antibody, the third detection line is coated with an NT-proBNP monoclonal antibody, and the quality control line is coated with a chicken IgY antibody; and the cTnT, NT-proBNP and D-dimer can be rapidly, accurately and quantitatively detected at the same time through one-time sampling.

Description

technical field [0001] The application relates to the technical field of fluorescence immunochromatography, in particular to a kit for quantitatively detecting cTnT / NT-proBNP / D-dimer and its application. Background technique [0002] Chest pain is a common and life-threatening condition. The causes of chest pain are complex and diverse, including acute coronary syndrome (Acute Coronary Syndrome, ACS), acute aortic dissection (Acute Aortic Dissection, AAD) and acute pulmonary embolism (Acute Pulmonary Embolism). Pulmonary Embolism, APE), among which ACS accounts for the highest proportion of these serious life-threatening diseases. In recent years, its incidence rate has been increasing year by year, and the risk of death of patients is also increasing. How to quickly and accurately diagnose and differentiate the etiology of ACS and other fatal chest pains has become the difficulty and focus of emergency treatment. All patients with chest pain need to undergo an electrocardi...

AI Technical Summary

Problems solved by technology

[0007] 1. The detection methods for cTnI, NT-proBNP and D-dimer include enzyme-linked immunoassay, fluorescent immunoassay, chemiluminescence, immunoturbidimetry and colloidal gold immunochromatography, etc., but the detection products and methods are mainly Limited to the detection of one or two of them, there are very few methods for simultaneous detection of the three

[0008] 2. Most myocardial injuries are treated with cardiac troponin I / T (cardiac troponin I / cardiac troponinT, cTnT / T), creatine kinase-MB (CK-MB), myoglobin (myoglobin, MYO), these three joint tests, although very important, are relatively single in terms of diagnosis. For patients with non-cardiac chest pain, these three tests have certain limitations, especially for emergency departments. challenged

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