Kit capable of being used detecting RNA methylation degree of H19 gene related to liver cancer and application for kit
A methylation and kit technology, applied in the field of detection kits for auxiliary diagnosis of malignant liver cancer, can solve the problems of low detection rate of early liver cancer, limitations, unsatisfactory diagnostic accuracy and sensitivity, etc.
Inactive Publication Date: 2020-06-05
YANGZHOU UNIV
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Primary liver cancer is one of the most common malignant tumors in the world. It ranks fifth in the incidence of clinical malignant tumors and ranks third in the mortality rate. However, the molecular mechanism of its pathogenesis is not yet fully understood.
At present, the screening of liver cancer mainly uses the combination of alpha-fetoprotein (AFP), ultrasound and other imaging diagnostic techniques, but ultrasound or imaging methods are limited by technical equipment or cost, and cannot be widely used
AFP is widely considered as the preferred serum marker for the diagnosis of liver cancer, but the detection rate of early liver cancer is not high, and its diagnostic accuracy and sensitivity are not satisfactory.
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[0024] The present invention will be further described below in conjunction with specific examples, but the protection scope of the present invention is not limited thereto.
[0025] 1. Collection of research objects
[0026] In this study, 55 surgical specimens (including corresponding paracancerous tissues) of liver cancer patients undergoing surgical resection in a hospital in Beijing were selected as the research objects. The diagnosis of all cases was based on the clinical data and imaging examination of the patients, and was confirmed by postoperative pathological diagnosis. Liver cancer tissue and paracancerous tissue (normal tissue at least 5 cm away from the cancer tissue) were surgically resected and immediately snap-frozen in liquid nitrogen and stored in a -80°C refrigerator for total RNA extraction. All research subjects signed informed consent.
[0027] 2. Total RNA extraction
[0028] Total tissue RNA was extracted by TRizol method, and the concentration of R...
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The invention relates to a kit capable of being used for detecting the RNA methylation degree of an H19 gene related to liver cancer and an application for the kit. The invention discloses the kit capable of being used for detecting the RNA methylation degree of the H19 gene related to the liver cancer and the application for the kit for the first time. The detection kit comprises a pair of RNA methylation specific amplification primers and an RNA methylation specific sequencing primer for the H19 gene; high RNA methylation of the H19 gene is remarkably related to the malignant degree of the liver cancer; and a new thought is provided for auxiliary diagnosis of malignant liver cancer through detection of the RNA methylation degree of the H19 gene.
Description
technical field [0001] The invention relates to a kit for detecting the degree of H19 gene RNA methylation related to liver cancer and an application thereof, belonging to the technical field of detection kits for auxiliary diagnosis of malignant liver cancer. Background technique [0002] Liver cancer is a tumor lesion in the liver, which is divided into primary liver cancer and secondary liver cancer. Primary liver cancer is one of the most common malignant tumors in the world. It ranks fifth in the incidence of clinical malignant tumors and ranks third in the mortality rate. However, the molecular mechanism of its pathogenesis is still not fully understood. At present, the screening of liver cancer mainly uses the combination of alpha-fetoprotein (AFP), ultrasound and other imaging diagnostic techniques, but ultrasound or imaging methods are limited by technical equipment or cost, and cannot be widely used. AFP is widely considered as the first choice serum marker for th...
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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154
Inventor 崔恒宓孙振
Owner YANGZHOU UNIV