Pharmaceutical composition for promoting estrus in dogs

A composition and drug technology, applied in the directions of drug combination, medical formula, vertebrate antigen components, etc., can solve the problems of low conception rate, inability to effectively induce estrus and ovulation in female dogs, and unsatisfactory effect.

Pending Publication Date: 2020-06-09
NANCHANG POLICE DOG BASE MINIST OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research of Xing Hua (2006) showed that a single small dose of PMSG (150IU~300IU) could not effectively induce estrus and ovulation in bitches, although a large dose (500IU) could effectively induce estrus and ovulation in bitches, but the conceptio

Method used

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  • Pharmaceutical composition for promoting estrus in dogs
  • Pharmaceutical composition for promoting estrus in dogs
  • Pharmaceutical composition for promoting estrus in dogs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: The immune effect of inhibin gene vaccine to dog

[0017] (1) Source of inhibin gene vaccine and its preparation method

[0018] The inhibin gene vaccine C501 (pVAX-S / INH-tpa) with attenuated Salmonella as the carrier was obtained from the Animal Reproduction Laboratory of the School of Animal Science and Technology of Huazhong Agricultural University. For related preparation methods, please refer to the literature: Yu Yaoyao et al., 2017). The preparation method is as follows: the correctly identified vaccine strain C501 (pVAX-S / INH-tpa) was added to 250ml LB liquid medium for expanded cultivation, and cultured with shaking at 200r / min at 37°C for 14h. Use the plasmid endotoxin removal kit to extract a large number of plasmids, measure the concentration of the plasmid, perform enzyme digestion and sequencing verification. According to needs, the obtained plasmid was diluted to different concentrations with normal saline, and stored at -20°C after aliquot...

Embodiment 2

[0037] Example 2: Inhibin Gene Vaccine Combined with PMSG on Canine Steroid Hormone Secretion

[0038] (1) The steps of preparation of vaccine, immunization and grouping of animals, and blood collection are the same as in Example 1.

[0039] (2) Determination of estrogen and progesterone:

[0040] The levels of estradiol and progesterone in canine blood were detected by double-antibody sandwich ELISA. Specific steps are as follows:

[0041] 1) Take out the ELISA plate, set a blank control well without adding any liquid; set up two wells for each standard point in turn, and add 50 μL of the corresponding standard product to each well; directly add 50 μL of the sample to be tested in each of the remaining detection wells.

[0042] 2) Add 50 μL of enzyme conjugate to each well (except for blank control wells), and then add 50 μL of antibody in the same order, mix thoroughly, attach self-adhesive to seal, and incubate at 37°C for 1 hour.

[0043] 3) Wash the plate manually and ...

Embodiment 3

[0049] Example 3: Effects of inhibin gene vaccine combined with PMSG on dog estrus and conception

[0050] Record the date of the first immunization according to the group, and closely observe each dog every day. When the vulva of the bitch is observed to be red, swollen, and dripping blood, it is judged to be in estrus. After 1 week of estrus, they were allowed to mate naturally. Record the date of estrus, breeding date, litter date and litter size. The results are shown in Table 3. According to Table 3, it can be seen that the estrus rate of bitches in T2 and T4 groups is the highest, reaching 66.7%, while the estrus rate of female dogs in the inhibin gene vaccine alone immunization group (T5) is the lowest, suggesting that inhibin gene vaccine and PMSG cooperate It can significantly increase the estrus rate of female dogs.

[0051] Table 3 Effects of different doses of inhibin gene vaccine combined with PMSG on estrus and conception in dogs

[0052]

[0053] Judging ...

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Abstract

The invention belongs to the technical field of animal reproduction, and in particular relates to a pharmaceutical composition for promoting estrus in dogs. The pharmaceutical composition can promoteestrus and the regulation of ovulation in dogs. The pharmaceutical composition is a pharmaceutical combination of an inhibin gene vaccine prepared with Salmonella as a carrier and horse chorionic gonadotropin (PMSG), which jointly induces estrus in dogs and assists reproduction of ovulation, so as to improve the reproduction ability of female dogs.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a pharmaceutical composition for promoting estrus in dogs and a pharmaceutical application thereof. The pharmaceutical composition can promote the regulation of estrus and ovulation in dogs. The pharmaceutical composition is a pharmaceutical combination of an inhibin gene vaccine prepared with Salmonella as a carrier and equine chorionic gonadotropin (PMSG), combined with assisted reproduction to induce estrus and ovulation in dogs, thereby improving the reproductive capacity of female dogs . [0002] technical background [0003] The preliminary work of the present invention is to prepare a kind of attenuated Salmonella called C501 (pVAX-S / INH-tpa), which is a kind of attenuated Salmonella with deletion of asd gene and crp gene, which contains non-resistance screening hepatitis B surface The fusion fragment of antigen S gene and inhibin gene (INH), its ma...

Claims

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Application Information

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IPC IPC(8): A61K38/24A61K39/00A61P15/08
CPCA61K38/24A61K39/0005A61P15/08A61K2300/00
Inventor 赵勤涛杨利国梁爱心熊前叶俊华祖卓鑫叶挺柱
Owner NANCHANG POLICE DOG BASE MINIST OF PUBLIC SECURITY
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