An ELISA kit for combined detection of autoantibodies for early-stage cardia adenocarcinoma screening

A technology of combined detection of autoantibodies, applied in the fields of molecular biology and oncology, to achieve strong balance, reduce misdiagnosis rate, and improve specificity
CN111323587BActive Publication Date: 2020-10-09THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
Publication Date
2020-10-09

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Abstract

The invention belongs to the technical field of medical biology, and specifically discloses an autoantibody joint detection ELISA kit for early cardia adenocarcinoma screening. The kit comprises a solid phase carrier and a tumor-associated antigen coated on the solid phase carrier, and the tumor-associated antigen is composed of P53, CDH1, NRG1, MDM2, RHOA and IVL. Furthermore, the kit also comprises a sample diluent, a second antibody, a second antibody diluent, positive control serum, negative control serum, a developing solution, a stop solution and a washing solution. The ELISA kit provided by the invention can effectively detect cardia adenocarcinoma, especially early cardia adenocarcinoma, has detection sensitivity as high as 86% and specificity as high as 88%, can be used for large-scale screening of asymptomatic people in cardia adenocarcinoma high-incidence areas, and is beneficial to screening and early discovery of asymptomatic high-risk people.
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Description

technical field

[0001] The invention belongs to the fields of molecular biology and oncology, and in particular relates to an ELISA kit for combined detection of autoantibodies for early cardia adenocarcinoma screening. Background technique

[0002] The cardia is located below the esophagogastric junction and most adjacent to the stomach, extending approximately 2 cm downward from the squamous-columnar junction. Stein et al. divided adenocarcinoma of the esophagogastric junction (AEG) into three types. Type I AEG is distal esophageal adenocarcinoma originating from special intestinal metaplasia; type II is true cardiac adenocarcinoma originating from Short segments of gastric cardia epithelium or intestinal metaplasia at the esophagogastric junction; type III is subcardia adenocarcinoma involving the esophagogastric junction and distal esophagus. Type II is the main type in my country, and the cardiac adenocarcinomas mentioned in this patent are all type II cardiac adenocar...

Claims

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