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Human testicular tissue suspension cryoprotectant and preparation method thereof

A technology of cryoprotectant and testis, which is applied in the preservation of human or animal bodies, germ cells, vertebrate cells, etc., can solve the problems of poor cryo-resuscitation effect and difficulty in finding, so as to improve clinical utilization rate, increase quantity, The effect of solving security risks

Inactive Publication Date: 2020-06-30
THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a cryoprotectant for human testicular tissue suspension and a preparation method thereof. The cryoprotectant is specially designed for the testicular tissue suspension, which can improve the cryopreservation effect of testicular sperm and effectively solve the problem of It has very important clinical application value to solve the problems of potential safety hazards, poor cryopreservation effect and difficulty in finding motile spermatozoa.

Method used

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  • Human testicular tissue suspension cryoprotectant and preparation method thereof
  • Human testicular tissue suspension cryoprotectant and preparation method thereof
  • Human testicular tissue suspension cryoprotectant and preparation method thereof

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Embodiment 1

[0024] A human testicular tissue suspension cryoprotectant, each 100mL includes the following raw materials by weight: 0.35g of sodium chloride, 0.03g of potassium chloride, 0.035g of calcium chloride, 0.008g of magnesium chloride, 0.004g of potassium dihydrogen phosphate, and sodium bicarbonate 0.2g, HEPES 0.3g, caffeine 0.035g, sodium pyruvate 0.0017g, pentoxifylline 0.102g, glycine 0.5g, glucose 0.08g and sucrose 1g; also includes sodium lactate 130μL and glycerin 10mL.

[0025] The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0026] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0027] (2) Add sodium lactate and glycerin to the mixed solution obt...

Embodiment 2

[0029] A human testis tissue suspension cryoprotectant, every 100mL human testis tissue suspension cryoprotectant comprises the following weight raw materials: sodium chloride 0.584g, potassium chloride 0.04g, calcium chloride 0.04g, magnesium chloride 0.01g, diphosphate Potassium hydrogen 0.005g, sodium bicarbonate 0.26g, HEPES 0.477g, caffeine 0.048g, sodium pyruvate 0.0028g, pentoxifylline 0.208g, glycine 1g, glucose 0.109g and sucrose 1.712g; also includes sodium lactate 188μL and glycerin 30mL.

[0030] The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0031] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0032] (2) Add sodium lactate and glycer...

Embodiment 3

[0034] A human testicular tissue suspension cryoprotectant, each 100mL includes the following raw materials by weight: 0.65g of sodium chloride, 0.045g of potassium chloride, 0.047g of calcium chloride, 0.0151g of magnesium chloride, 0.007g of potassium dihydrogen phosphate, and sodium bicarbonate 0.32g, HEPES 0.55g, caffeine 0.058g, sodium pyruvate 0.0035g, pentoxifylline 0.555g, glycine 1.3g, glucose 0.209g and sucrose 2.5g; also includes sodium lactate 280μL and glycerin 65mL.

[0035]The preparation method of above-mentioned human testis tissue suspension cryoprotectant, comprises the following steps:

[0036] (1) Weigh successively sodium chloride, potassium chloride, calcium chloride, magnesium chloride, potassium dihydrogen phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose, add After the ultrapure water is dissolved, a mixed solution is obtained;

[0037] (2) Add sodium lactate and glycerin to the mixed soluti...

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Abstract

The invention discloses a human testicular tissue suspension cryoprotectant and a preparation method thereof. The human testicular tissue suspension cryoprotectant comprises the following raw materials: sodium chloride, potassium chloride, calcium chloride, magnesium chloride, monopotassium phosphate, sodium bicarbonate, HEPES, caffeine, sodium pyruvate, pentoxifylline, glycine, glucose and sucrose. The human testicular tissue suspension cryoprotectant also comprises sodium lactate and glycerol. The invention further discloses the preparation method of the human testicular tissue suspension cryoprotectant and a freezing method adopting the human testicular tissue suspension cryoprotectant. The cryoprotectant is specially designed for a testicular tissue suspension, the testicular sperm cryopreservation and resuscitation effect can be improved, the problems that potential safety hazards exist, the cryopreservation and resuscitation effect is poor, and movable testicular sperms are not easy to find are effectively solved, and the human testicular tissue suspension cryoprotectant has quite important clinical application value.

Description

technical field [0001] The invention relates to the technical field of cryoprotectant and its preparation, in particular to a human testicular tissue suspension cryoprotectant and a preparation method thereof. Background technique [0002] With the development of social industrialization, environmental pollution is becoming more and more serious, and the proportion of infertility is increasing. The incidence rate among couples of childbearing age is as high as 9.3%, and among them, the male factor accounts for about 30-50%. Among the male factors, The proportion of patients with azoospermia is as high as 10-20%. In 1992, the emergence of intracytoplasmic sperm injection (ICSI) technology realized the wish of male patients with oligospermia to have offspring. In 1993, Schoysman used testicular sperm to perform ICSI and achieved success. Clinical application brings good news. Clinically, on the basis of excluding genetic diseases, patients with azoospermia usually undergo te...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N5/076
CPCA01N1/0226C12N5/061
Inventor 李福平鲜泱刘博蒋敏柳莎莎刘晓赵文瑞
Owner THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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