Human BCR/ABL gene T315I mutation detection and analysis

A technology for humans, testing methods, applied in the field of molecular biology, which can solve problems such as ineffectiveness

Inactive Publication Date: 2020-07-03
山东瑞启智博生物科技有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

When the dose of TKI is increased or the second-generation TKI is replaced, most of the mutant patients are clinically effective for the above changes, but patients with BCR / ABLT315I mutations are not effective for the above drugs

Method used

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  • Human BCR/ABL gene T315I mutation detection and analysis
  • Human BCR/ABL gene T315I mutation detection and analysis
  • Human BCR/ABL gene T315I mutation detection and analysis

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Embodiment Construction

[0022] The mutation detection and analysis of the human BCR / ABLT315I gene is based on the principle of amplification block mutation, which can accurately detect the mutant DNA with a content as low as 0.5%, that is, only 100 copies of the mutant can be detected; specific detection In the background of a higher copy of the wild plasmid, there is still no false positive.

[0023] The ABL T315I mutation of the BCR-ABL fusion gene is clinically used as the main indicator for CML and Ph+ALL drug treatment guidance, and has important guiding significance for the screening of CML and Ph+ALL drug resistance and clinical scientific medication.

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Abstract

The invention provides a kit for human BCR / ABLT 315 I mutation and a preparation method and application thereof. The application of the kit has guiding significance for CML and Ph+ALL drug resistancescreening and clinical scientific drug use, wherein the ABL T315I mutation of BCR-ABL fusion gene is clinically used as a main indicator for CML and Ph+ALL drug treatment guidance.

Description

technical field [0001] The invention relates to gene detection technology in the field of molecular biology, in particular to the detection and analysis of human BCR-ABL gene T315I mutation, and provides a kit for human BCR / ABLT315I mutation. Background technique [0002] The Philadelphia chromosome is t(9;22)(q34;q11) formed by the mutual translocation of chromosomes 9 and 22, which encodes an abnormal protein "BCR-ABL" fusion protein. Due to the different breaking points of ABL on chromosome 9, three fusion genes were produced, encoding three fusion proteins P190, P210, and P230 with different molecular weights. Among them, 95% of chronic myelogenous leukemia (CML) and 20%-40% of acute lymphoblastic leukemia (ALL) can detect the characteristic Ph chromosome and its molecular marker BCR-ABL fusion gene. [0003] CML is a malignant proliferative disease of the hematopoietic system. Its global incidence rate is 1.6 / 100,000 people, and the incidence rate in my country is 0.39...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6858C12Q1/6851
CPCC12Q1/6851C12Q1/6858C12Q1/6886C12Q2600/106C12Q2600/156C12Q2600/166C12Q2531/113C12Q2535/137C12Q2545/101
Inventor 钟建韩国英
Owner 山东瑞启智博生物科技有限公司
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