High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes

A chromatographic material, a high-purity technology for high-purity chromatographic materials that contain ionizable modifiers for retention of acidic analytes, to resolve issues such as sample or diluent detection incompatibilities

Active Publication Date: 2020-07-10
WATERS TECH CORP
View PDF23 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, these current methods can suffer from negative results due to sample or diluent limitations, the need for specialized equipment, or incompatibility with mass spectrometry (MS) detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes
  • High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes
  • High purity chromatographic materials comprising an ionizable modifier for retention of acidic analytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0302] will formula (O 1.5 SiCH 2 CH 2 SiO 1.5 ) (SiO 2 ) 4 (Prepared according to the method described in US Patent 6,686,035) BEH porous hybrid particles (15g, Waters Corporation, Milford, MA; 6.5%C; SSA=186m 2 / g; SPV=0.79cm 3 / g; ) was refluxed in toluene (100 mL, Fisher Scientific, Fairlawn, NJ) for 1 hour using a Dean-Stark separator. Reaction 1a used 7.2 g of BEH material. After cooling, add Component A silane additives including aminopropyltriethoxysilane (APTES, Gelest Inc., Morrisville, PA), 2-(2-(trichlorosilyl)ethyl)pyridine (2PE, Gelest Inc., Morrisville, PA), 2-(4-pyridylethyl)triethoxysilane (4PE, Gelest Inc., Morrisville, PA), N-trimethoxysilylpropyl- N,N,N-trimethylammonium chloride (QPTMS, 50% solution in methanol, Gelest Inc., Morrisville, PA) or chloropropyltrimethoxysilane (CPTMS, Gelest Inc., Morrisville, PA). The reaction was heated to reflux for 1 hour. After cooling, imidazole (Aldrich, Milwaukee, WI) and octadecyldimethylchlorosilane (Comp...

Embodiment 2

[0306] The material from Example 1 was modified with trimethylchlorosilane (TMCS, Gelest Inc., Morrisville, PA) using imidazole (Aldrich, Milwaukee, WI) in refluxing toluene (100 mL) for 4 hours. The reaction was then cooled, and the product was filtered and washed sequentially with water, toluene, 1:1 v / v acetone / water, and acetone (all solvents were obtained from J.T. Baker), then dried at 80° C. under reduced pressure for 16 hours. The reaction data are listed in Table 2.

[0307] Table 2

[0308] product precursor Granules (g) TMCS(g) Imidazole (g) ％C 2a 1a 7.2 1.49 1.12 13.96 2b 1b 15.9 3.27 2.48 14.22 2c 1c 15.0 3.00 2.25 14.33 2d 1d 15.0 3.11 2.34 13.97 2e 1e 15.6 3.30 2.44 12.93 2f 1f 15.0 3.00 2.25 14.57 2g 1g 15.0 3.11 2.34 14.19 2h 1h 15.0 3.00 2.25 14.19 2i 1i 15.0 3.11 2.34 13.80 2j 1j 15.0 3.00 2.25 13.83 2k 1k 15.0 2.99 2.25 13.12 2l 1l 1...

Embodiment 3

[0310] will formula (O 1.5 SiCH 2 CH 2 SiO 1.5 ) (SiO 2 ) 4 (Prepared according to the method described in US Patent 6,686,035) BEH porous hybrid particles (Waters Corporation, Milford, MA; 6.5% C; SSA=182-185m 2 / g; SPV=0.72-0.76cm 3 / g; ) was refluxed for 1 hour in toluene (5 mL / g, Fisher Scientific, Fairlawn, NJ) using a Dean-Stark separator. After cooling, add Component A silane additives including aminopropyltriethoxysilane (APTES, Gelest Inc., Morrisville, PA), 2-(4-pyridylethyl)triethoxysilane ( 4PE, Gelest Inc., Morrisville, PA) or diethylphosphorylethyltriethoxysilane (DEPS, Gelest Inc., Morrisville, PA) or 2-(4-chlorosulfonylphenyl)ethyltrichloro Silane (SPETCS, 50% solution in toluene, Gelest Inc., Morrisville, PA). The reaction was heated to reflux for 1 hour. After cooling, imidazole (Aldrich, Milwaukee, WI) and octadecyltrichlorosilane (Component B, ODTCS, Aldrich, Milwaukee, WI) were added. The reaction was then heated to reflux for 16 hours. The pro...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle sizeaaaaaaaaaa
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The present invention provides the use of charged surface reversed phase chromatographic materials along with standard reversed-phase LC and mass spectrometry compatible conditions for the retention,separation, purification, and characterization of acidic, polar molecules, including, but not limited to, organic acids, alpha-amino acids, phosphate sugars, nucleotides, other acidic, polar biologically relevant molecules. The chromatographic materials of the invention are high purity chromatographic materials comprising a chromatographic surface wherein the chromatographic surface comprises a hydrophobic surface group and one or more ionizable modifier.

Description

[0001] related application [0002] This application claims the benefit of U.S. Provisional Application No. 62 / 563,334, filed September 26, 2017, the entire disclosure of which is incorporated by reference. Background technique [0003] Chromatographic retention of acidic, polar analytes such as organic acids, sugars, and phosphorylated compounds is difficult to achieve with current chromatographic techniques. Current methods often use ion-pair reagents in combination (Luo et al., Journal of Chromatography B, Vol. 1147, 2007, pp. 153-164 and Lu et al., Analytical Chemistry, 2010, Vol. ), ion chromatography (IC) or hydrophilic interaction chromatography (HILIC). (Bajad et al., Journal of Chromatography A, Vol. 1125, 2006, pp. 76-88). In addition, the use of derivatization can be performed as a means of reducing the polarity of molecules (Tan et al., Analytical Biochemistry, Vol. 465, 2014, pp. 134-147), allowing retention, separation, and subsequent detection of these analyt...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/32B01D15/36B01J20/283B01J20/287
CPCB01D15/327B01D15/36B01J20/287B01D15/3847B01J20/28061B01J20/28073B01J20/28076B01J20/283B01J20/288B01J2220/54B01J2220/80
Inventor M.A.劳伯P.赖因维尔J.N.费尔蔡尔德B.奥肯德吉N.L.劳伦斯D.沙赫
Owner WATERS TECH CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap