Establishment and evaluation method of underactive bladder animal model

Abstract

The invention relates to an establishment and evaluation method of an underactive bladder animal model. The method comprises the following steps: selecting SPF male SD rats, and randomly dividing therats into five groups under proper feeding conditions, wherein the first group is a sham group, and sham group rats only expose the bladder neck without ligation; and establishing a partial bladder outlet obstruction (PBOO) model by a modified method for the 2nd to 5th groups, wherein in the second to fifth groups, the second group is a control group, the PBOO model is established just by the modified method, and the third group to fifth groups are respectively given TGF-beta1 at different doses for multi-point injection at a bladder serosal layer. The invention provides the establishment andevaluation method of the underactive bladder animal model. The PBOO model established by the modified method is combined with the TGF-beta1 multi-point injection at the bladder serosal layer, so thatthe UAB model is established successfully, and the establishment method has the advantages of simple and controllable operation, high success rate and stable model.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for establishing and evaluating an animal model of bladder hypoactivity. Background technique [0002] Underactive bladder (UAB) is a common, chronic and complicated urinary system disease related to bladder detrusor underactivity (DU). Patients are usually accompanied by obvious lower urinary tract symptoms, such as waiting for urination, strenuous urination, slow urine flow, incomplete bladder emptying, and patients also have varying degrees of abnormal bladder filling sensation, which can easily lead to increased bladder residual urine volume and chronic urinary retention Etc., significantly increased the risk of urinary system infection, calculus and renal failure. Among elderly men over 70 years old, the incidence rate can be as high as 48%. Moreover, 89% of the population is currently unaware of their own DU or UAB, so the actual incidence may be higher. Currently, th...

AI Technical Summary

Problems solved by technology

However, the sleeve method and the silver clip method have a large free range, large trauma, and high animal mortality. The thread ligation method generally needs to insert a catheter through the urethra, which is easy to damage the urethra, causing problems such as urethral edema and fibrosis.

In addition, it still takes a long time for BOO to evolve into UAB, and individual differences are large, which makes it difficult to establish a stable UAB animal model and the success rate is low

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