Medicinal composition for inducing stem cells to differentiate into cartilage and application of medicinal composition

A stem cell differentiation and composition technology, applied in the field of medicine, can solve problems such as high price, and achieve the effects of promoting proliferation, inducing and promoting differentiation into chondrocytes, and having strong pharmacological effects

Active Publication Date: 2020-07-17
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The object of the present invention is to provide a pharmaceutical composition and its application, the pharmaceutical composition has the ability to effectively induce bone marrow mesenchymal stem cells to differentiate into cartilage, to achieve The purpose of treating osteoarthritis, and the pharmaceutical composition of the present invention replaces the chemical substances or cytokines or hormones that induce bone marrow mesenchymal stem cells to differentiate into cartilage, and jointly induces BMSCs to differentiate into cartilage, overcoming the problem of high price and safety. Deficiencies that cannot be widely used in clinical practice, and the anti-inflammatory, anti-oxidant, and anti-tumor activities of puerarin and tetrandrine can improve the success rate of stem cell transplantation, reduce rejection, and reduce the risk of stem cell differentiation into tumors; and this The inventive pharmaceutical composition has a remarkable effect of inducing bone marrow mesenchymal stem cells to differentiate into chondrocytes, provides a new source of BMSCs-inducing drugs for stem cell transplantation in the treatment of osteoarthritis diseases, and provides a new drug source for the treatment of osteoarthritis diseases drug source

Method used

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  • Medicinal composition for inducing stem cells to differentiate into cartilage and application of medicinal composition
  • Medicinal composition for inducing stem cells to differentiate into cartilage and application of medicinal composition
  • Medicinal composition for inducing stem cells to differentiate into cartilage and application of medicinal composition

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 is prepared medicinal liquid

[0037] 1. Prepare puerarin solution

[0038] Accurately weigh 4.36mg puerarin, dissolve in 1mL DMSO, and prepare 10-2 M puerarin mother liquor, the mother liquor was subpackaged and stored in a refrigerator at -20°C, filtered through a disposable sterile syringe filter with a pore size of 0.22 μm before use, and diluted with deionized water as a solvent in sequence to a concentration of 10 μM, 1 μM, Five different concentrations of puerarin, 0.1 μM, 0.01 μM, and 0.001 μM, were administered to the solution.

[0039] 2. Preparation of tetrandrine solution

[0040] Accurately weigh 6.28mg tetrandrine, dissolve in 1mL DMSO, and prepare 10 -2 M powder tetrandrine mother liquor, divide the mother liquor, store in -20°C refrigerator, filter it through a 0.22μm pore size disposable sterile syringe filter before use, and use deionized water as a solvent to dilute it successively to a concentration of 10μM and 5μM , 2.5μM, 1.25μM, 0....

Embodiment 2

[0050] Example 2 Isolation, culture and identification of SD rat bone marrow mesenchymal stem cells

[0051] 1. Experimental animals and reagents

[0052] Healthy SPF-grade Sprague Dawley (SD) male rats, 4-6 weeks old, were purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (permit number: SCXK (Beijing) 2016-0006).

[0053] Certified Foetal Bovine Serum Qualtified for human MSC (fetal bovine serum for stem cell culture grade), Bioilgical Industries, the United States; MEM-ALPHA medium, Bioilgical Industries, the United States; DMEM high-sugar, low-sugar phenol red-free medium, Sigma-Aldrich Company, the United States ; 0.25% trypsin digest (containing 2.21mM EDTA), Corning, the United States; CD29 (PE Hamster Anti-Rat CD29), CD45 (PE-Cy TM 5 Mouse Anti-Rat CD45), CD54 (PE Mouse Anti-Rat CD54), CD90 (APC Mouse Anti-Rat CD90 / MouseCD90.1), CD11b (FITC Mouse Anti-Rat CD11b), BD Biosciences, the United States; RecombinantHuman BMP -2. Recombinant Human...

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Abstract

The invention discloses a medicinal composition for inducing stem cells to differentiate into cartilage and an application of the medicinal composition. The medicinal composition comprises puerarin and tetrandrine. The invention also discloses an application of the medicinal composition in preparing medicaments or health-care products for inducing bone marrow mesenchymal stem cells (BMSCs) to differentiate into cartilage cells and an application of the medicinal composition in medicaments for treating osteoarthritis. According to the invention, puerarin and tetrandrine are proportioned to replace cytokines or hormones to jointly induce BMSCs to differentiate to cartilage, so that the defect that medicines cannot be widely applied to clinical practice due to high price and safety problems is overcome; and the puerarin and the tetrandrine are proportioned to improve the success rate of stem cell transplantation, reduce rejection reactions and reduce the risk of stem cells differentiate to tumors. The medicine composition provided by the invention has obvious effects in inducing BMSCs to differentiate into cartilage cells, has dose dependence, is suitable for popularization and application, and provides a new BMSCs induction medicine source for treating osteoarthritis diseases.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a drug combination for inducing bone marrow mesenchymal stem cells to differentiate into cartilage and its application in treating osteoarthritis. Background technique [0002] Osteoarthritis (OA) is a chronic joint disease characterized by degeneration of articular cartilage and secondary bone hyperplasia. The disease is more common in middle-aged and elderly people, and women are more common than men; it mainly occurs in knee joints, hip joints, lumbosacral spinal joints and first metatarsophalangeal joints with heavy loads. Its pathological features are degenerative changes of articular cartilage, destruction of tissue structure, subchondral bone sclerosis, reactive hyperplasia of joint margins and subchondral bone, and osteophyte formation. Although the research on osteoarthritis has been very in-depth, traditional therapy can only improve the symptoms of osteoa...

Claims

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Application Information

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IPC IPC(8): A61K31/4748A61K31/352A61P19/02A61P29/00
CPCA61K31/4748A61K31/352A61P19/02A61P29/00A61K2300/00
Inventor 崔元璐陈昳冰
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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