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Method for extracting cannabidiol by purple sulfur bacteria fermentation and application of cannabidiol

A technology of purple sulfur bacteria and cannabidiol, which is applied in chemical instruments and methods, pharmaceutical formulations, topical antibacterial agents, etc., can solve the problems of high cost, low efficiency, and difficulty in realizing industrialized scale-up production, etc. simple effect

Active Publication Date: 2020-07-17
云南植源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the supercritical extraction technology has a high extraction rate and no solvent residue, the equipment investment is large, the efficiency is low, the cost is high, the energy consumption is high, and it is difficult to realize industrial scale-up production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for extracting cannabidiol by fermentation of purple sulfur bacteria, comprising the steps of:

[0026] (1) The flowers and leaves of industrial hemp are vacuum-dried at 55°C at low temperature until the water content is ≤5%, then pulverized, and passed through a 100-mesh sieve to obtain industrial hemp powder;

[0027] (2) Add 10 times its mass of water to the industrial hemp powder obtained in step (1) to obtain a mixed solution, pour the fermented purple sulfur bacteria fermentation solution into the mixed solution, wherein the purple sulfur bacteria fermentation solution and the industrial hemp aqueous solution The mass ratio is 1:100. In a closed environment, use sunlight or a 60-watt incandescent bulb to ferment and cultivate at 30°C for 30 hours to obtain a cannabis fermentation liquid;

[0028] (3) Filter the cannabis fermentation liquid obtained in step (2), remove the residue, concentrate under reduced pressure, remove some impurities in the cannabis ...

Embodiment 2

[0039] A method for extracting cannabidiol by fermentation of purple sulfur bacteria, comprising the steps of:

[0040] (1) Drying the flowers and hemp bran of industrial hemp in vacuum at low temperature at 60°C until the water content is ≤5%, then pulverize them, and pass through a 100-mesh sieve to obtain industrial hemp powder;

[0041] (2) Add 12 times its mass of water to the industrial hemp powder obtained in step (1) to obtain a mixed solution, pour the fermented purple sulfur bacteria fermentation solution into the mixed solution, wherein the purple sulfur bacteria fermentation solution and the industrial hemp aqueous solution The mass ratio is 1:150. In a closed environment, use sunlight or a 60-watt incandescent bulb to ferment and cultivate at 35°C for 26 hours to obtain a cannabis fermentation liquid;

[0042] (3) Filter the cannabis fermentation liquid obtained in step (2), remove the residue, concentrate under reduced pressure, remove impurities in the cannabis ...

Embodiment 3

[0053] A method for extracting cannabidiol by fermentation of purple sulfur bacteria, comprising the steps of:

[0054] (1) Drying the flowers, leaves and hemp bran of industrial hemp in vacuum at low temperature at 65°C until the water content is ≤5%, then pulverizing them, and passing through a 100-mesh sieve to obtain industrial hemp powder;

[0055] (2) Add 15 times its mass of water to the industrial hemp powder obtained in step (1) to obtain a mixed solution, pour the fermented purple sulfur bacteria fermentation solution into the mixed solution, wherein the purple sulfur bacteria fermentation solution and the industrial hemp aqueous solution The mass ratio is 1:200. In a closed environment, use sunlight or a 60-watt incandescent bulb to ferment and cultivate at 40°C for 24 hours to obtain a cannabis fermentation liquid;

[0056] (3) Filter the cannabis fermentation liquid obtained in step (2), remove the residue, concentrate under reduced pressure, remove impurities in ...

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Abstract

The invention belongs to the technical field of chemical drug intermediate extraction, and discloses a method for extracting cannabidiol by purple sulfur bacteria fermentation. The method comprises the following steps: by using one or more of flowers, leaves and hemp bran of industrial hemp as raw materials, drying and pulverizing to obtain a hemp raw material of which the water content does not exceed 5%; adding water and a cultured purple sulfur bacterium fermentation liquor into a transparent organic glass tank filled with the crushed hemp raw material, and irradiating, sealing and standingfor 24-30 hours to obtain a hemp fermentation liquor; filtering the fermentation liquor to remove residues; performing low-temperature reduced-pressure concentration to obtain a primary extract containing cannabidiol; and removing impurities by using macroporous resin, and purifying by using silica gel column chromatography to obtain cannabidiol with the purity of more than 80%. The method is simple to operate and low in cost, and has good popularization and application values. The invention also discloses application of cannabidiol as a main component, and alkannin, essential oil and other components are added or not added, so that the traditional Chinese medicine composition has the effects of treating infantile red buttocks, eczema, urination rash, saliva rash and the like.

Description

technical field [0001] The invention relates to the technical field of extraction of chemical drug intermediates, in particular to a method for extracting cannabidiol by fermentation of purple sulfur bacteria and its application. Background technique [0002] Industrial hemp, also known as hemp, is a 1-year-old herbaceous plant of the Cannabis family Cannabis genus, which has a wide range of applications. Cannabidiol, a non-addictive component in hemp, has the functions of protecting nerves, improving memory, anti-inflammation, sterilization, analgesia, anti-anxiety, anti-psychotic, and anti-oxidation, and is widely used in medicines, health products, functional drinks, and cosmetics and other fields have high research value. [0003] At present, the extraction methods of cannabidiol mainly include supercritical extraction and organic solvent extraction. Compared with the organic solvent extraction method, the use of microbial fermentation to extract cannabidiol can reduce...

Claims

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Application Information

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IPC IPC(8): C07C37/68C07C37/82C07C39/23A61K36/9066A61K36/61A61K36/30A61K31/05A61P31/02A61P17/00
CPCA61K31/05A61K36/30A61K36/61A61K36/9066A61P17/00A61P31/02C07C37/685C07C37/82C07C2601/16C07C39/23A61K2300/00
Inventor 丁瑞孙秋
Owner 云南植源生物科技有限公司
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