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Culture medium for dictyophora rubrovolvata cultivated species and preparation method of culture medium

A technology for Pseudomonas erythrinatus and a culture medium, which is applied in the field of edible fungus seed production, can solve the problems such as the mycelium of P.

Inactive Publication Date: 2020-07-24
贵州织金毫米农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the relatively high temperature in summer and autumn (mainly from July to October in production), the microbial activities are frequent. After the preparation of the medium, the microorganisms multiply rapidly and the pH value drops rapidly. If the storage time is long, the acid in the medium will accumulate more. The pH value will drop, and the pH value will drop below 5.5 and cause the medium to become rancid, and the mycelia of Dictyophora can not grow normally and may die

Method used

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  • Culture medium for dictyophora rubrovolvata cultivated species and preparation method of culture medium
  • Culture medium for dictyophora rubrovolvata cultivated species and preparation method of culture medium
  • Culture medium for dictyophora rubrovolvata cultivated species and preparation method of culture medium

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preparation example Construction

[0022] The present invention also provides a preparation method for the culture medium described in the above technical solution, comprising: mixing the raw materials with water and then sterilizing to obtain the culture medium; the mass ratio of the total mass of the raw materials to water is 1:1.3~ 1.6 。

[0023] In the present invention, the sterilization time is preferably 2 hours, the sterilization pressure is preferably 0.11-0.14 MPa, and the sterilization temperature is preferably 121-125°C.

Embodiment 1

[0026] Based on the percentage of the total mass of the medium for cultivating Dictyophora edulis, 73% of sawdust, 19.8% of wheat bran, 3% of corn flour, 2% of soybean flour, 1% of sugar, 0.7% of gypsum, 0.3% of quicklime, diphosphate Mix 0.1% potassium hydrogen and 0.1% magnesium sulfate, adjust the water content to 60%, stack at an outdoor temperature of 25°C for 6 hours, use high pressure (0.11-0.14Mpa) to sterilize for 2 hours, use it in bags for 1 hour, and heat up for 1 hour. More than 10 repetitions, the pH value after sterilization is 6-7, wherein the pH value is 83.5% when the pH value is 6-6.5, and the pH value is 16.5% when the pH value is 6.5-7. After being inoculated with the mycelia of Dictyophora edulis, the germination rate of the mycelium reached 100%.

Embodiment 2

[0028] Based on the percentage of the total mass of the medium for cultivating Dictyophora edulis, 73% of sawdust, 19.7% of wheat bran, 3% of corn flour, 2% of soybean flour, 1% of sugar, 0.5% of gypsum, 0.6% of quicklime, and diphosphate Mix 0.1% potassium hydrogen and 0.1% magnesium sulfate, adjust the water content to 60%, stack at an outdoor temperature of 25°C for 12 hours, use high pressure (0.11-0.14Mpa) to sterilize for 2 hours, use it in bags for 1 hour, and heat up for 1 hour. More than 10 repetitions, the pH value after sterilization is 6-7, wherein the pH value of 6-6.5 is 65.4%, and the pH value of 6.5-7 is 34.6%. After being inoculated with the mycelia of Dictyophora edulis, the germination rate of the mycelium reached 100%.

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Abstract

The invention provides a culture medium for dictyophora rubrovolvata cultivated species and a preparation method of the culture medium, and belongs to the technical field of edible fungus seed production. The culture medium comprises the following raw materials in parts by weight: 73-80 parts of sawdust, 14.7-19.8 parts of wheat bran, 1 part of sugar and 0.5-0.8 part of gypsum. The culture mediumfor the dictyophora rubrovolvata cultivated species provided by the invention is allowed to stand for 6-12 h at the temperature of 25 DEG C or higher and then sterilized, the pH value of the culture medium is still 6-7, and after dictyophora rubrovolvata hyphae are inoculated, the germination rate of the dictyophora rubrovolvata hyphae reaches 100%.

Description

technical field [0001] The invention belongs to the technical field of edible fungus seed production, and in particular relates to a culture medium of Dictyophora cultivar and a preparation method thereof. Background technique [0002] In Guizhou, Dictyophora edulis is generally planted from December to May of the following year, and most growers plant Dictyophora edulis from late December to February of the following year, so the time for cultivating Dictyophora edulis is generally 7 From July to December, the golden seed production period of cultivars is from July to October. The culture medium of Dictyophora edulis requires a pH value of 5.5-7.5, and the optimum growth pH value is 6-7. Gypsum is generally used as the pH value of the culture medium in the seed production process. value regulator. The general formula is: wood chips 78%, wheat bran 20%, sugar 1%, gypsum 1%, medium water content 60%. Due to the relatively high temperature in summer and autumn (mainly from J...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G24/25A01G24/23A01G24/20A01G24/10C05G3/00
CPCA01G24/10A01G24/20A01G24/23A01G24/25C05B7/00C05G3/00C05D3/00C05D3/02C05D5/00C05F5/002C05F11/00
Inventor 杨念龙王波刘德宾
Owner 贵州织金毫米农业科技发展有限公司
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