Tissue culture method for obtaining kelp filaments and application of tissue culture method

A tissue culture, filamentous technology, applied in applications, horticultural methods, botanical equipment and methods, etc., can solve problems such as germplasm degradation

Active Publication Date: 2020-07-28
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method of using different varieties of male and female gametophyte clones and hybrid breeding is still not separated from the met

Method used

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  • Tissue culture method for obtaining kelp filaments and application of tissue culture method
  • Tissue culture method for obtaining kelp filaments and application of tissue culture method
  • Tissue culture method for obtaining kelp filaments and application of tissue culture method

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Effect test

Embodiment 3

[0034] Example 3 The enlarged cultured kelp filaments, microscopic examination is a mixed state of male and female, and the female filaments account for the vast majority, respectively hybridized with normal haploid male and female gametophytes, according to the conventional process of kelp seedling cultivation, to obtain normal products Seedlings, and can complete temporary cultivation and cultivation in the sea.

[0035] According to the different hybrid combinations in Table 1, the seedling raising experiment was carried out. The seedling raising conditions of the four combinations were the same. After two and a half months of cultivation, samples were taken and photographed respectively. Figure 5 . Depend on Figure 5 It can be seen that the combination of kelp-induced filaments and kelp haploid male gametophyte (No. 46) has obvious advantages over other combinations in terms of growth speed and seedling volume. The kelp filaments induced by the method of the present in...

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Abstract

The invention discloses a tissue culture method for obtaining kelp filaments and application of the tissue culture method. The method comprises the following steps of placing infant kelp spore explants in a culture dish, adding a PESI culture solution, culturing in an illumination incubator with the culture temperature of 15 +/-2 DEG C, the illumination intensity of 1500-2000 Lux, and the photoperiod of 10 L: 14 D; replacing the PESI culture solution once every 7-10 days in two months before the PESI culture solution; replacing once every 15-30 days in the third month to the fourth month; andthen replacing kelp explants once every 50-60 days until brown filamentous body clusters around the kelp explants can be seen under a microscope. According to the kelp filaments obtained through the method, on one hand, breed conservation can be carried out through tissue culture of pure line first filial generation filaments, and on the other hand, a large number of pure line filaments can be generated by enlarging cultivation to carry out conventional cultivation and seedling culture.

Description

technical field [0001] The invention relates to a tissue culture method for obtaining kelp filaments and an application method of the obtained kelp filaments. The invention belongs to the technical field of seaweed tissue culture and application. Background technique [0002] In the biological natural classification system, kelp belongs to Phaeophyta, Phaeophyta, Laminaria, Laminaceae, and Laminaria. Its life history has obvious alternation of generations, that is, gametophyte generation and sporophyte generation. The morphological difference between gametophyte and sporophyte is significant. The sporophyte is huge and the gametophyte generally requires the help of a microscope to observe clearly, and the gametophyte can live independently without the mother camp. In the 1970s, Chinese scholar Fang Zongxi discovered that under certain conditions, kelp gametophytes can only grow vegetatively, forming asexual reproduction lines, also called clones, and using cloning to carry...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/005A01H4/001
Inventor 田萍萍姜黎明彭捷李言刘延岭武瑞娜史良孙娟梁广津罗世菊李晓捷
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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