Momordica charantia peptide for wrinkle relieving and repairing and preparation process thereof

A preparation process and peptide technology are applied in the field of balsam pear peptide for anti-wrinkle repair and its preparation technology, which can solve the research on skin wrinkle repair effect without balsam pear peptide, the low efficiency of polypeptide extraction, and the anti-wrinkle repair effect of balsam pear peptide Excellent and other problems, to achieve the effect of anti-glycation wrinkles, improve economic efficiency and reduce wrinkles

Inactive Publication Date: 2020-07-28
ZHEJIANG XITAI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, in the prior art, the extraction efficiency of the polypeptide in bitter melon is not high,

Method used

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  • Momordica charantia peptide for wrinkle relieving and repairing and preparation process thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation technology of bitter gourd peptide for anti-wrinkle repair comprises the following steps:

[0038] (1) balsam pear seeds are shelled, the seeds without rot are selected, and crushed to 40 orders to obtain balsam pear seed powder;

[0039] (2) The bitter melon seed powder is subjected to supercritical CO 2 fluid extraction, the supercritical CO 2 The condition of fluid extraction is CO 2 The flow rate is 15kg / h, the extraction pressure is 35Mpa, the extraction temperature is 35°C, the extraction time is 2h, the oil is removed, and the defatted bitter melon seed powder is obtained;

[0040] (3) Add defatted balsam pear seed powder into water 10 times the weight of the defatted balsam pear seed powder, adjust the pH to 9.6 with 0.1mol / L aqueous sodium hydroxide solution, stir and extract at 50°C for 4 hours at a speed of 150r / min, and Centrifuge at 6000r / min for 20min, separate the precipitate from the supernatant, and obtain the extraction slag and extr...

Embodiment 2

[0043] The preparation technology of bitter gourd peptide for anti-wrinkle repair comprises the following steps:

[0044] (1) balsam pear seeds are shelled, the seeds without rot are selected, and crushed to 40 orders to obtain balsam pear seed powder;

[0045] (2) The bitter melon seed powder is subjected to supercritical CO 2 fluid extraction, the supercritical CO 2 The condition of fluid extraction is CO 2 The flow rate is 15kg / h, the extraction pressure is 35Mpa, the extraction temperature is 35°C, the extraction time is 2h, the oil is removed, and the defatted bitter melon seed powder is obtained;

[0046] (3) Add defatted balsam pear seed powder into water 10 times the weight of the defatted balsam pear seed powder, adjust the pH to 9.6 with 0.1mol / L aqueous sodium hydroxide solution, stir and extract at 50°C for 4 hours at a speed of 150r / min, and Centrifuge at 6000r / min for 20min, separate the precipitate from the supernatant, and obtain the extraction slag and extr...

Embodiment 3

[0050] The preparation technology of bitter gourd peptide for anti-wrinkle repair comprises the following steps:

[0051] (1) balsam pear seeds are shelled, the seeds without rot are selected, and crushed to 40 orders to obtain balsam pear seed powder;

[0052] (2) The bitter melon seed powder is subjected to supercritical CO 2 fluid extraction, the supercritical CO 2 The condition of fluid extraction is CO 2 The flow rate is 15kg / h, the extraction pressure is 35Mpa, the extraction temperature is 35°C, the extraction time is 2h, the oil is removed, and the defatted bitter melon seed powder is obtained;

[0053] (3) Add defatted balsam pear seed powder to the water of 10 times of the weight of the defatted balsam pear seed powder, add bromelain of 12% of the weight of the defatted balsam pear seed powder, add L-sodium ascorbate of 0.2% of the weight of the defatted balsam pear seed powder, at 50° C. Stir at 150r / min for enzymolysis for 12h, incubate at 95°C for 8min to inact...

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Abstract

The invention relates to a momordica charantia peptide for wrinkle relieving and repairing and a preparation process thereof. The preparation process comprises the following steps: (1) hulling momordica charantia seeds, and performing grinding; (2) performing supercritical CO2 fluid extraction; (3) adjusting the pH value of the mixture to 9-10 with a sodium hydroxide aqueous solution having the concentration of 0.05-0.15mol/L, performing stirring and extracting at 30-60 DEG C for 2-6 hours, performing centrifuging, separating precipitate from supernatant liquid to respectively obtain extractedslag and an extracted liquid; and (4) performing ultrafiltration on the extracted liquid with an ultrafiltration membrane with molecular weight cut-off being 8000-15000Da, and performing freeze-drying. According to the momordica charantia peptide for wrinkle relieving and repairing and the preparation process, through a great deal of researches, a practical and efficient preparation process is found out, and a novel momordica charantia peptide for wrinkle relieving and repairing is developed, so that the economic benefit of the momordica charantia peptide for wrinkle relieving and repairing can be increased; the method for preparing the momordica charantia peptide is simple in process, and the momordica charantia peptide can be used as a cosmetic raw material and health food additive andcan achieve the effects of resisting saccharification and relieving wrinkles.

Description

technical field [0001] The invention relates to a bitter melon peptide, in particular to a bitter melon peptide for anti-wrinkle repair and a preparation process thereof. Background technique [0002] Bitter melon (Momordica charantia L.) is a Cucurbitaceae plant. The whole plant, including the fruit, has a bitter taste. The leaf margins are tooth-shaped. The fruit is oblong. The tender fruit is emerald green and orange-yellow when ripe. , can be used as vegetables again, and is widely planted in the world. The fruit is bitter in nature and cold in taste. It has the effects of clearing away heat, improving eyesight and detoxification. It can be applied topically to treat trauma, and taken internally for deworming, regulating menstruation, anti-virus, treating measles and hepatitis. [0003] According to "Compendium of Materia Medica" and "Southern Yunnan Materia Medica", bitter gourd has the functions of clearing away heat and refreshing heat, nourishing blood and Qi, tonif...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/14C12P21/06A61K8/64A61Q19/00A61Q19/08
CPCA61K8/645A61Q19/00A61Q19/08C07K1/145C07K1/30C07K1/34C07K1/36C12P21/06Y02P20/54
Inventor 潘文桥
Owner ZHEJIANG XITAI BIOTECHNOLOGY CO LTD
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