Millet spore culture medium for fermentation production of lovastatin and preparation method of millet spore

A culture medium preparation, lovastatin technology, applied in the direction of using spores, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of high labor intensity, unfavorable large-scale production, inconvenient operation, etc., to reduce contamination The effect of reducing the chance of bacteria, reducing labor intensity, and ensuring stability

Inactive Publication Date: 2015-04-29
SHANDONG LUKANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of the spore bacteria suspension is to add an appropriate amount of sterile water to the mature eggplant bottle, wash the spores on the surface with an inoculation needle, and then use a sterile pipette to suck the spore liquid into a sterile triangular flask containing glass beads. Shake and smash, and then merge the dispersed spore suspension obtained in such a plurality of triangular flasks into another larger sterile triangular flask, and then connect it to the first-level seed tank; this process is in the actual operation process There are many disadvantages in this method, such as high labor intensity, inconvenient operation, more glassware used, and complicated operation, which makes it difficult to ensure sterility
In short, this process greatly increases the workload and the probability of contamination, which is extremely unfavorable for large-scale production; therefore, it is necessary to seek a simpler and more effective preparation process for strains used in tanks

Method used

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  • Millet spore culture medium for fermentation production of lovastatin and preparation method of millet spore

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this embodiment, the process of culturing and preparing rice spores is described by taking the water increase amount of millet reaching 20% ​​as an example, including the following steps:

[0038] (1) Weigh 200g (W1) of millet, 60ml of water, and 3g of corn steep liquor, mix the corn steep liquor and water evenly and pour into a pot to heat, then pour the millet into it and stir-fry until the weight of the millet reaches 250g and take out ;

[0039] (2) Steam the fried millet at 100°C for 15 minutes, and then rub it up;

[0040] (3) after the steamed millet is aired to 240g (W2), the millet is divided into shake bottles, 25g / bottle;

[0041] (4) steam the shake flask that has been divided into millet at 100°C for 20 minutes; then take it out after sterilizing at 115°C for 40 minutes, then shake the bottom of the shaker flask to disperse the millet evenly;

[0042] (5) After each shake flask was aired to room temperature, the spore concentration was 1.2×10 8 0.5ml ...

Embodiment 2

[0046] In this embodiment, the process of culturing and preparing rice spores is described by taking the water increase amount of millet reaching 25% as an example, including the following steps:

[0047] (1) Weigh 200g (W1) of millet, 80ml of water, and 2g of corn steep liquor, mix the corn steep liquor and water evenly and pour into a pot to heat, then pour the millet into it and stir-fry until the weight of the millet reaches 260g and take out ;

[0048] (2) Steam the fried millet at 105°C for 15 minutes, and then rub it up;

[0049] (3) after the steamed millet is dried to 250g (W2), the millet is divided into shake bottles, 25g / bottle;

[0050] (4) steam the shake flask that has been divided into millet at 95°C for 25 minutes; then take it out after sterilizing at 121°C for 30 minutes, then shake the bottom of the shaker flask to disperse the millet evenly;

[0051] (5) After each shake flask was aired to room temperature, the spore concentration was 2.6×10 8 0.5 ml of...

Embodiment 3

[0055]In this embodiment, the process of culturing and preparing rice spores is described by taking the water increase amount of millet reaching 50% as an example, including the following steps:

[0056] (1) Weigh 200g (W1) of millet, 120ml of water, and 4g of corn steep liquor, mix the corn steep liquor and water evenly and pour into a pot to heat, then pour the millet into it and stir-fry until the weight of the millet reaches 310g and take out ;

[0057] (2) Steam the fried millet at 100°C for 20 minutes, then rub it up;

[0058] (3) after the steamed millet is dried to 300g (W2), the millet is divided into shake bottles, 20g / bottle;

[0059] (4) Steam the shake flask that has been divided into millet at 90°C for 30 minutes; then take out after sterilizing at 123°C for 25 minutes, then shake the bottom of the shaker flask to disperse the millet evenly;

[0060] (5) After each shake flask was aired to room temperature, the spore concentration was 1.44×10 8 0.2 ml of Monas...

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Abstract

The invention relates to a millet spore culture medium for fermentation production of lovastatin, and belongs to the field of biological pharmacy. The millet spore culture medium for fermentation production of lovastatin is mainly prepared from the raw materials of millet and corn steep liquor. The invention further provides a method adopting the millet spore culture medium to prepare the millet spore, wherein the culture for the method is a microbial culture for fermentation production of lovastatin. The culture medium provided by the invention is simple and effective; the preparation method of the millet spore can be used for producing a lot of high-quality spores, so that the labor intensity of workers can be lowered, and meanwhile the microbiological contamination probability can be greatly reduced.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to an oatmeal medium for fermentation production of lovastatin and a method for culturing and preparing high-quality spores by using the medium. Background technique [0002] Lovastatin is a hydroxymethylglutaryl coenzyme A reductase inhibitor (HMG-CoA-RI), which inhibits the activity of HMG-CoA reductase competitively and blocks the synthesis of endogenous cholesterol, thereby regulating lipids. Lipid-lowering effect. Lovastatin was first discovered from the fermentation broth of Aspergillus terreus by Japanese Endo and Albort in 1979 and 1980, developed by Merck Co, and approved by the FDA in 1987. The first HMG-CoA-RI on the market. Since its listing, lovastatin has become the drug of choice for clinical treatment and prevention of hyperlipidemia. In recent years, many new pharmacological activities of lovastatin have been discovered, such as anti-cancer, anti-oxidation, and imp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C12N3/00C12R1/66C12R1/645
Inventor 白芳静王文笙田晓梅戴晓艳谢寅伟金伟闫婷婷吴洪宁张爽马艳茹吕利静
Owner SHANDONG LUKANG PHARMA
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