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Method for identifying or assisting to identify black shank resistance of to-be-detected Nicotiana tabacum

A tobacco black shank and auxiliary identification technology, which is applied in the field of identification or auxiliary identification of the resistance of tobacco black shank to be tested, can solve the problem of less utilization and achieve high accuracy

Active Publication Date: 2020-07-28
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But blackleg resistance genes in 'Beinhart-1000' are closely associated with cigar traits, and resistance in this line is less utilized in Burley and flue-cured varieties

Method used

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  • Method for identifying or assisting to identify black shank resistance of to-be-detected Nicotiana tabacum
  • Method for identifying or assisting to identify black shank resistance of to-be-detected Nicotiana tabacum
  • Method for identifying or assisting to identify black shank resistance of to-be-detected Nicotiana tabacum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] Example 1, fine mapping of flue-cured tobacco black shank resistance QTL

[0062] 1. Construction of RIL groups

[0063] 1. Crossing the highly resistant flue-cured tobacco variety 'Yunyan 85' and the susceptible flue-cured tobacco variety 'Dabaijin 599' to obtain F 1 generation seeds.

[0064] 2. After completing step 1, plant F 1 Generation seeds, self-pollination, get F 2 generation seeds.

[0065] 3. After completing step 2, plant F 2 177 F7:8-9RIL families were obtained by single-seed transmission method. 177 F7:8-9RIL families constituted the RIL population.

[0066] 2. Tobacco black shank resistance analysis

[0067] The tobacco materials to be tested were 'Yunyan 85', 'Dabaijin 599' and 177 RIL families.

[0068] 1. The inventors of the present invention were registered in Zhucheng, Weifang City, Shandong Province in 2017 (ZC2017 for short), in Zhucheng, Weifang City, Shandong Province in 2018 (ZC2018 for short), in Zhucheng, Weifang City, Shandong Provi...

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Abstract

The invention discloses a method for identifying or assisting to identify the black shank resistance of to-be-detected Nicotiana tabacum. The method includes the following steps: detecting whether thegenotype of to-be-detected Nicotiana tabacum is a genotype I or a genotype II, wherein the Nicotiana tabacum with the genotype I has or suspiciously has black shank resistance and the Nicotiana tabacum with the genotype II does not have or suspiciously does not have black shank resistance. The Nicotiana tabacum with the genotype I is the Nicotiana tabacum whose genotype based on SNP locus 1 is aTT homozygous type and / or genotype based on SNP locus 2 is a GG homozygous type; the Nicotiana tabacum with the genotype II is the Nicotiana tabacum whose genotype based on the SNP locus 1 is a CC homozygous type and / or genotype based on the SNP locus 2 is an AA homozygous type; the SNP locus 1 is the nucleotide 101 from the 5'end of SEQ ID NO: 1 in the genome of the Nicotiana tabacum; and the SNPlocus 2 is the nucleotide 101 from the 5'end of SEQ ID NO: 2 in the genome of the Nicotiana tabacum. Thus, the method has important values.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying or assisting in identifying the resistance of tobacco black shank to be tested. Background technique [0002] Tobacco (Nicotianatabacum L.) is an important economic crop and a model system for studying plant-pathogen interactions. Black shank is a disease caused by soil-borne Phytophthora Nicotianae that seriously affects tobacco production and can widely infect various plants. Compared with chemical control strategies, locating disease-resistant genes and cultivating disease-resistant varieties is the safest, most economical and effective method to reduce the economic loss of this pathogen. [0003] The physiological races of tobacco black shank that have been reported so far include races 0, 1, 2 and 3, and the physiological races of tobacco black shank in China are mainly races 0 and 1. The disease resistance genes php and phl derived from wi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6869
CPCC12Q1/6895C12Q1/6869C12Q2600/13C12Q2600/156
Inventor 龚达平陈明丽向小华高军平郝俊杰蒲文宣徐秀红解敏敏
Owner TOBACCO RES INST CHIN AGRI SCI ACAD