Double-channel quadrature phase microimaging sampling system

Abstract

The invention provides a dual-channel quadrature phase microimaging sampling system, which comprises a pentaprism, a laser emission device, a first image acquisition device and a second image acquisition device, and the pentaprism is internally provided with a hollow channel. A first light beam enters the pentaprism and then horizontally enters the sample in the hollow channel, part of the first light beam is horizontally transmitted out to form object light of a horizontal channel, and the other part of the first light beam forms reference light of a vertical channel. After a second light beam enters the pentaprism, part of the second light beam is horizontally transmitted out through the semi-transparent and semi-reflective prism surface of the pentaprism to form reference light of the horizontal channel, and the other part of the second light beam forms object light of the vertical channel. The first image acquisition device is used for acquiring an interference amplification imageof object light and reference light of the horizontal channel, and the second image acquisition device is used for acquiring an interference amplification image of object light and reference light ofthe vertical channel. According to the invention, double light sources with the same quality are realized, and the matching performance between channels can be improved.

Description

technical field [0001] The invention relates to the technical field of optical three-dimensional morphology imaging or the field of cell three-dimensional morphology microscopic imaging, in particular to a dual-channel orthogonal phase microscopic imaging sampling system. Background technique [0002] In subject research and engineering applications, there are many micro-scale phase bodies (that is, transparent to visible light) that require 3D morphology microscopic imaging, especially the detection of cell morphology has important scientific research and clinical application significance. Since cells are phase bodies, and the scale is on the order of microns, they are active, so they are particularly dependent on the level of phase microscopy imaging technology. For example: common leukemia, ischemic anemia, retinopathy and other diseases can be known from the morphological analysis results of white blood cells; diabetes, sepsis, malaria, arteriosclerosis, myocardial infar...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a dual-channel quadrature-phase microscopic imaging sampling system, which solves the three deficiencies of the traditional dual-optical-path quadrature-phase microscopic imaging optical path. The imbalance of the two channels will cause different systematic errors in the two orthogonal channels, which will affect the accuracy of shape reconstruction; the second is that the traditional imaging is often a static slide sample, and the flow channel method is used here , and ensure the normal incidence of both beams on the sampling channel, which can effectively improve the imaging sampling speed. The third is that the traditional orthogonal dual-channel phase microscopic imaging optical path structure is complex, there are many components, and the maintenance cost is high. Pentaprism is used as the optical path The optical path structure under modulation will effectively solve such problems

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