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Inflorescence-tissue-culture rapid propagation method for Wushan epimedium

A technology of tissue culture rapid propagation and Epimedium, applied in the field of plant tissue culture, to achieve the effects of low production cost, stable genetics, and avoiding accumulation from generation to generation

Active Publication Date: 2020-08-04
WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on tissue culture and rapid propagation of epimedium, and there are few reports on tissue culture and rapid propagation of inflorescences as explants.

Method used

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  • Inflorescence-tissue-culture rapid propagation method for Wushan epimedium
  • Inflorescence-tissue-culture rapid propagation method for Wushan epimedium
  • Inflorescence-tissue-culture rapid propagation method for Wushan epimedium

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1: a kind of Wushan Epimedium inflorescence tissue culture rapid propagation method, its steps are as follows:

[0044] (1) Sterilization of inflorescences: collect Epimedium Wushanense in early spring and germinate fresh and disease-free inflorescences 10 or 12 or 14 or 16 or 18 or 20 cm long on the soil surface, put the explant material in a 500ml large beaker, cover it with gauze and place it in tap water Rinse under water for 1 hour, drain, transfer to ultra-clean workbench and sterilize with 75% alcohol solution at 120rpm for 30s, take it out and rinse with sterile water 3 times to remove residual alcohol; then sterilize with 0.1% mercuric chloride for 5 minutes, sterile Rinse with water 4 times, and then place on sterilized filter paper to remove residual water for later use.

[0045] (2) Inducing explants to generate callus: cut the sterilized inflorescence into 1.5cm long sections in an ultra-clean bench, and then inoculate it on the callus induction ...

Embodiment 2

[0050] Embodiment 2: a kind of Wushan Epimedium inflorescence tissue culture rapid propagation method, its steps are as follows:

[0051] (1) Sterilization of inflorescences: collect Epimedium Wushanense in early spring and germinate fresh and disease-free inflorescences 10 or 12 or 14 or 16 or 18 or 20 cm long on the soil surface, put the explant material in a 500ml large beaker, cover it with gauze and place it in tap water Rinse under water for 1 hour, drain, transfer to ultra-clean workbench, sterilize with 75% alcohol solution at 120rpm for 45s, take it out and rinse with sterile water 4 times to remove residual alcohol; then disinfect with 0.1% mercuric chloride for 5 minutes, no Rinse with bacterial water for 5 times, and then place on sterilized filter paper to remove residual water for later use.

[0052] (2) Inducing explants to generate callus: cut the sterilized inflorescence into 1cm-long sections in an ultra-clean bench, and then inoculate them on the callus indu...

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Abstract

The invention discloses an inflorescence-tissue-culture rapid propagation method for Wushan epimedium. The method comprises the steps of disinfecting inflorescence, inducing an explant to generate calli and differentiate adventitious buds or cluster buds, then, inducing buds to form fibrils, and carrying out seedling hardening and seedling raising, thereby finally completing a complete process forforming healthy complete plants. The differentiation rate of differentiated germination of the calli reaches up to 83.7%, and the multiplication coefficient of the cluster buds can reach 5.1. The method is easy to operate and has the advantages of high propagation rate, low cost, short cycle, robust young seedlings and the like, a foundation is provided for large-scale production of the epimedium, and the method has a certain promoting action on protecting and utilizing epimedium resources.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and more specifically relates to a tissue culture rapid propagation method using Epimedium wushanense inflorescences as explants. Background technique [0002] Epimedium wushanense Ying is a plant of the genus Epimedium in the family Berberidaceae, and its medicinal part is the dry leaf. It is one of the most widely used and long-standing Chinese medicinal materials in China. my country is the main supplier of Epimedium medicinal materials and extracts in the world. Modern pharmacology shows that with the development of new medicines such as anti-cancer and nerve protection using Epimedium as raw materials, the demand for Epimedium has further increased. As a result, the reserve of wild resources of Epimedium has decreased sharply, resulting in a serious contradiction between the supply and demand of Epimedium medicinal materials. Obviously relying on the wild resources of Epimediu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 张燕君于东悦梁琼黄天悦于淑霞王兰香袁芳
Owner WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI
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