Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of rice transcription factor BTF3 in identification of japonica rice and indica rice and method for identifying japonica rice and indica rice

A technology of rice transcription factor and transcription factor, applied in the application field of rice transcription factor BTF3 in the identification of japonica rice and indica rice, can solve the problems of insufficient speed and precision, and achieve the effects of high accuracy, high throughput and high specificity

Inactive Publication Date: 2020-08-04
SOUTH CHINA AGRI UNIV +2
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the defect that the existing method for distinguishing japonica rice and indica rice is not fast enough and accurate enough, the present invention provides an application of rice transcription factor BTF3 in differentiating japonica rice and indica rice, using transcription factor BTF3 as a molecular marker, using transcription factor BTF3 to identify There are polymorphic differences between japonica and japonica rice, and the japonica and indica rice can be quickly identified by detecting the transcription factor BTF3

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of rice transcription factor BTF3 in identification of japonica rice and indica rice and method for identifying japonica rice and indica rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] 1. Materials and methods

[0063] 1.1 Experimental materials

[0064] The experimental materials used as the molecular marker method for identifying indica and japonica rice varieties of the present invention include common wild rice and Nivara wild rice (Nivara wild rice); indica varieties 1-7 are respectively 93-11, Nante, Guang Luai 4, Zhenshan 97, Minghui 63, Huangsizhan and Huanghuazhan; Japonica rice varieties 1-7 are Nipponbare, Taichung 65, Zhonghua 11, Nongken 58, Yunjing 23, Ewan 3 and Jindao respectively number 1.

[0065] 1.2 Experimental method

[0066] step 1)

[0067] (1) Take about 0.5g of rice leaves, cut them into small pieces, put them in a pre-cooled mortar, add liquid nitrogen, grind the leaves into powder, put them in a 2mL centrifuge tube, and add 800μL 2×CTAB extraction buffer , Mix well, water bath at 65℃ for 30min;

[0068] (2) Add equal volume of chloroform, isoamyl alcohol and absolute ethanol (76:4:20), shake for 10min, and mix well;

[0069] (3) Cen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a rice transcription factor BTF3 in identification of japonica rice and indica rice and a method for identifying the japonica rice and the indica rice, and relates to the technical field of rice variety identification. According to the application of the rice transcription factor BTF3 in identification of japonica rice and indica rice, the polymorphism difference of the transcription factor BTF3 in the japonica rice and indica rice is utilized, and germplasm resources of the indica rice and japonica rice can be conveniently and accurately distinguished from each other for rice cultivated varieties. The invention also provides a method for identifying japonica rice and indica rice by using the rice transcription factor BTF3, and the rapid identificationof the japonica rice and indica rice can be realized only by PCR and enzyme digestion. The identification method disclosed by the invention is low in cost, high in flux, high in specificity (namely high accuracy) and suitable for production practice.

Description

Technical field [0001] The present invention relates to the technical field of rice variety identification, in particular to the application of rice transcription factor BTF3 in identifying japonica rice and indica rice and a method for identifying japonica rice and indica rice. Background technique [0002] Rice varieties are mainly cultivated in Asia. According to a series of phenotypic differences, they are divided into Indica (Oryzasaliva subsp Indica) and Japonica (Oryza saliva subsp Japanica). According to different geographical planting conditions and production needs, China has developed and cultivated thousands of indica and rice varieties. Japonica rice varieties. To ensure high and stable yields, seed purity of rice varieties is one of the most critical factors. The purity of rice varieties is traditionally identified in different growth periods such as seedling stage, heading stage, and ripening stage. There are three main aspects of the identified traits, namely pl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/156
Inventor 王曼陈杰湖张群宇刘伟林锐松
Owner SOUTH CHINA AGRI UNIV