Saline alkali soil microbial remediation microbial agent and preparation method and application thereof

A technology for microbial remediation and saline-alkali soil, which is applied in the field of saline-alkaline soil microbial remediation bacterial agents and its preparation, and can solve the problems of no symbiosis and synergy between bacteria and substrates, unfavorable proliferation and survival of bacteria, unfavorable survival of soil microorganisms, etc. , to achieve green and healthy development, improve nutrient use efficiency and cultivated land quality, and improve soil fertility

Active Publication Date: 2020-08-07
INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the soil conditioning products in the prior art are designed from a single point of view, either microorganisms or minerals, and the effect is single; microbial soil conditioning products are produced by using a single organic raw material or a single bacterial strain for adsorption, which is not conducive to the bacterial

Method used

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  • Saline alkali soil microbial remediation microbial agent and preparation method and application thereof
  • Saline alkali soil microbial remediation microbial agent and preparation method and application thereof
  • Saline alkali soil microbial remediation microbial agent and preparation method and application thereof

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preparation example Construction

[0029] As a preferred embodiment, the preparation method of composite bacterial agent in the present invention comprises:

[0030] (1) The jelly-like bacillus liquid and the Streptomyces jingyang liquid obtained by shaking the flask culture are connected to the seed tank for mixed fermentation according to the inoculum size of 2.8-3.2%, and cultivated at a temperature of 30° C.-35° C. for 30-35 hours;

[0031] (2) The Bacillus subtilis liquid obtained by shaking the flask culture is inserted into the seed tank according to the inoculation amount of 2.8-3.2%, and cultivated at a temperature of 30-37° C. for 24-26 hours;

[0032] (3) the jelly-like bacillus obtained in the step (1), the Streptomyces jingyang mixed bacterial liquid by the inoculation amount of 10%~20%, the Bacillus subtilis bacterial liquid obtained in the step (2) by the 5%~10 % of the inoculation amount is connected to the production tank, and cultured at 30-35°C for 38-40 hours at the same time;

[0033](4) S...

Embodiment 1

[0118] Compound bacteria preparation:

[0119] Process flow:

[0120] 1. Strain liquid fermentation

[0121] 1.1 Shake flask culture:

[0122] (1) Culture medium of Streptomyces jingyang

[0123] Medium: 20 grams of soluble starch

[0124] KNO 3 1.0g

[0125] K 2 HPO 4 0.5 grams

[0126] MgSO 4 .7H 2 O 0.5 g

[0127] NaCl 0.5g

[0128] FeSO 4 .7H 2 O 0.01 g

[0129] 1000ml of distilled water

[0130] pH7.3

[0131] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the slant to corresponding shake flasks, and cultivate them at 26°C and 200rpm for 42h.

[0132] (2) Cultivation of Bacillus subtilis liquid

[0133] Culture medium: 200 grams of potatoes

[0134] 20 grams of sucrose

[0135] 1000ml of distilled water

[0136] pH 6.3

[0137] Inject 150ml of culture medium into a 500ml Erlenmeyer flask; use 5ml of sterile water to transfer the strains on the slant to corresponding shake flasks, and ...

Embodiment 2

[0189] Repair strong saline alkaline soil bacteria agent:

[0190] Fully mix 50 parts of edible fungus residue, 20 parts of humic acid powder, 15 parts of sulfur (industrial grade), and 15 parts of the composite bacterial agent prepared in Example 1, extrude and granulate, and obtain the bacterial agent for repairing strong saline alkaline soil .

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Abstract

The invention relates to the technical field of microbial soil remediation, and discloses a saline alkali soil microbial remediation microbial agent, which is prepared from the following raw materialsin parts by weight: 40 to 50 parts of edible fungus residue, 20 to 30 parts of humic acid powder, 6 to 10 parts of sulfur, and 10 to 20 parts of a composite microbial agent containing streptomyces jingyangensis, bacillus subtilis and bacillus mucilaginosus. According to the microbial soil remediation agent provided by the invention, various microbial living life activities and metabolites, organic matters and minerals are combined, so that the pH and salt content of soil are reduced, the granular structure and organic matter content of the soil are increased, the soil fertility is improved, and the soil ecology is recovered.

Description

technical field [0001] The invention relates to the technical field of soil remediation, in particular to a microbial remediation agent for saline-alkali soil and its preparation method and application. Background technique [0002] Land salinization is an important worldwide problem faced by human beings, and the development and management of saline-alkali land is a hot spot concerned by relevant countries and institutions in the world. The formation of saline-alkali land is due to natural or man-made reasons, which increase the underground water level, increase the salinity, dry climate, and increase evaporation, resulting in enhanced salinization or alkalization of the soil surface, and surface salinity or alkalization. aggravated phenomenon. Saline-alkali soil is formed under certain natural or artificial conditions. The essence of its formation is the redistribution of various soluble salts in the horizontal and vertical directions on the ground, so that the salt gradu...

Claims

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Application Information

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IPC IPC(8): C05F17/20C05G3/80C12N1/20C09K17/40C09K101/00C09K109/00C12R1/125C12R1/465C12R1/07
CPCC05D9/00C05F17/20C05G3/80C09K17/40C12N1/20C09K2101/00C09K2109/00C05F5/00C05F5/002C05F11/02Y02W30/40
Inventor 江丽华石璟王梅李洪梅徐钰杨岩李妮胡斌
Owner INST OF AGRI RESOURCES & ENVIRONMENT SHANDONG ACADEMY OF AGRI SCI
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