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Application of microorganisms as diagnostic marker of endometrial cancer and kit

A technology of endometrial cancer and diagnostic markers, applied in the field of molecular markers, can solve problems such as the breakdown of microbial balance

Pending Publication Date: 2020-08-07
FUJIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the anatomical characteristics of the female reproductive tract, it is an open structure, vulnerable to the invasion of various pathogens. Although it has a certain defense function, when the environment changes, drug stimulation, the host's own level and immune status change, the external When the source pathogen enters the reproductive tract or the flora in the female reproductive tract will also change, the microbial balance in the host reproductive tract will be broken, and the original physiological state will turn into a pathological state, resulting in the occurrence of diseases

Method used

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  • Application of microorganisms as diagnostic marker of endometrial cancer and kit
  • Application of microorganisms as diagnostic marker of endometrial cancer and kit
  • Application of microorganisms as diagnostic marker of endometrial cancer and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 Extraction of total DNA in endometrial tissue (SDS method)

[0030] 1) Lysis: Take 50 mg of ground tissue in liquid nitrogen, add EDTA (0.5M, pH8.0) and 550 μl SDS, mix well, add Proteinase K, invert and mix well, incubate at 55°C for 2 hours, and gently vortex to help Completely lysed.

[0031] 2) Centrifugation: Centrifuge at 12000rpm for 5min, absorb the supernatant, add protein precipitation solution (5M NaCl), vortex gently for 10sec, place at -20°C for several minutes, then centrifuge at 12000rpm for 10min.

[0032] 3) Precipitation: Pipette the supernatant into a new EP tube, centrifuge at 12,000 rpm for 5 minutes, and there may be a small amount of protein precipitate at the bottom of the tube. Pipette the supernatant after centrifugation into a new EP tube, add isopropanol, mix well and place at -20°C for 20 minutes, then centrifuge at 12,000 rpm for 10 minutes. Pour off the liquid, taking care not to pour out the precipitate.

[0033] 4) Washing: Wash twi...

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Abstract

The invention belongs to the technical field of molecular markers, and provides application of microorganisms as a diagnostic marker of endometrial cancer and a kit. The microorganisms comprise microorganisms of Rothia, Rhodoplanes, Lachnospira, Campylobacter, Succinivibrio and Pseudoramibacter_Eubacterium. Microorganisms of Rothia, Rhodoplanes, Lachnospira, Campylobacter, Succinivibrio and Pseudoramibacter_Eubacterium can be used as the diagnostic marker of endometrial cancer.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, and in particular relates to the application and kit of microorganisms as diagnostic markers for endometrial cancer. Background technique [0002] With the development of bacterial 16S rRNA gene sequencing technology, the relationship between microbial flora and disease has become a research hotspot. Domestic and foreign studies have found that the incidence of gynecological malignancies is closely related to the microbial flora of the reproductive tract. Due to the anatomical characteristics of the female reproductive tract, it is an open structure, vulnerable to the invasion of various pathogenic bacteria. Although it has a certain defense function, when the environment changes, drug stimulation, the host's own level and immune status change, the external When the source pathogen enters the reproductive tract or the flora in the female reproductive tract will also change, the microbi...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/689C12Q1/6869C12Q1/04C12N15/11
CPCC12Q1/6886C12Q1/689C12Q1/6869C12Q2600/158
Inventor 何斐卢婉婷黄玉秀胡志坚林征
Owner FUJIAN MEDICAL UNIV
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