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Microorganism enhancement method for enriching cadmium in hyperaccumulative plants and the composite suspension used

A complex and biochar technology, applied in the direction of chemical instruments and methods, restoration of contaminated soil, application, etc., can solve problems such as plant physiological damage, achieve low cost, improve the ability of cadmium enrichment, and the method is simple Effect

Active Publication Date: 2021-02-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But at the same time, under heavy metal stress, the time required for phytoremediation and the physiological damage to plants under high pollution conditions are the main problems faced by phytoremediation.

Method used

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  • Microorganism enhancement method for enriching cadmium in hyperaccumulative plants and the composite suspension used
  • Microorganism enhancement method for enriching cadmium in hyperaccumulative plants and the composite suspension used
  • Microorganism enhancement method for enriching cadmium in hyperaccumulative plants and the composite suspension used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, a kind of preparation method of bacterial strain-carbon-based saline complex suspension, carries out following steps successively:

[0031] 1), prepare the thalline of cadmium-resistant bacteria M6:

[0032] Inoculate the cadmium-resistant bacteria M6 into the liquid LB medium, shake the bacteria to the logarithmic phase at a temperature of 28°C and a speed of 200r / min under aerobic conditions, and adjust the OD value with a UV spectrophotometer according to the growth curve of the bacteria , to ensure that the bacterial liquid cfu ≥ 1 × 10 8 cells / mL, centrifuge at 8000r / min for 10min, discard the supernatant, and obtain the bacterial cells for use.

[0033] 2) Collect pine and bamboo from the wild, first place them in an oven at 60°C for drying and dehydration for 20 hours, then dry them in an oven at 48°C for 28 hours, take them out, crush them with a crusher, and pass through a 1mm sieve to obtain pine and bamboo particles;

[0034] The pine and bamb...

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Abstract

The invention discloses a preparation method of a bacterial strain-carbon-based saline complex suspension, comprising the following steps: preparing the thallus of cadmium-resistant bacteria M6; preparing biomass charcoal by using pine and bamboo; adding pine and bamboo biomass charcoal into physiological saline, adjusting pH to neutral; add bacteria to the obtained charcoal-based saline and cultivate to obtain a strain-charcoal-saline complex suspension. The present invention also provides a microbial enhancement method for enriching cadmium in hyperaccumulative plants: planting sedum sedum with ore in cadmium-contaminated soil, and applying strain-carbon-based salt water complex suspension every 8-12 days. The microbial enhancement method of the present invention is based on increasing the field activity of the cadmium-resistant bacterial agent, thereby improving the ability of hyperaccumulator plants to enrich cadmium. The method is simple, low in cost, and has broad application prospects.

Description

technical field [0001] The invention relates to a microbial strengthening method for superaccumulating phytoremediation of soil cadmium pollution, and the used bacterial strain-carbon-based salt water complex suspension. Background technique [0002] With the continuous advancement of my country's industrialization process, the problem of heavy metal pollution in soil has become increasingly serious and has attracted more and more attention. At the same time, people's research on pollution control methods has become more and more in-depth. Phytoremediation has attracted widespread attention because of its low price, simple operation, less environmental disturbance, and more thorough treatment effects. But at the same time, under heavy metal stress, the time required for phytoremediation and the physiological damage to plants under high pollution conditions are the main problems faced by phytoremediation. Therefore, with the deepening of research, it has been found that the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40B09C1/10B09C1/08C12N11/14
CPCB09C1/08B09C1/10B09C1/105B09C2101/00C09K17/40C12N11/14
Inventor 张奇春王京文王浣荷金树权
Owner ZHEJIANG UNIV