Method for producing high-selenium-content protein through yeast fermentation by using exogenous metallothionein

Abstract

The invention discloses a method for producing high-selenium-content protein by constructing yeast fermentation by using exogenous metallothionein. Yeast is used as an original strain to design a primer pair YES2-MT2 and a primer pair MT2-yes2-F, genetic transformation is carried out to obtain genetically engineered bacteria, and the genetically engineered bacteria are fermented to produce the high-selenium-content protein. According to the invention, Saccharomyces cerevisiae is used as an original bacterial strain, the genetically engineered bacteria are obtained through exogenous gene genetic transformation, and the genetically engineered bacteria are subjected to fermentation production and separation purification to obtain the high-selenium-content protein. The total efficiency of converting inorganic selenium into selenoprotein reaches up to 22%, and industrial production can be achieved.

Description

technical field [0001] The invention relates to a method for using exogenous metallothionein to construct yeast to ferment and produce high-selenoproteins. Specifically, Saccharomyces cerevisiae is used as the original strain to obtain genetically engineered bacteria through genetic transformation of exogenous genes, and the genetically engineered bacteria are then fermented Produce high selenoproteins. Background technique [0002] Selenium is an essential trace element for living organisms. It has the functions of anti-oxidation, anti-aging, improving immunity, preventing cardiovascular diseases and cancer. However, people in many areas of my country suffer from insufficient selenium nutrition and need to supplement selenium-containing foods. Studies have shown that organic selenium compounds can be better absorbed and utilized by the human body. The organic selenium synthesized by microbial fermentation has the characteristics of rapidity, stability and high efficiency. ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the problems in the prior art that the high-efficiency enrichment method of organic selenium in cells is induced by heavy metals or directly expresses the MT gene construction vector in the host, and the induction by heavy metals will cause difficulties in later stage purification, etc. However, direct expression of the MT gene construct vector in the host will face defects such as the difficulty of detecting transformants, and provides a method for using exogenous metallothionein to construct yeast fermentation to produce high selenoproteins