97 results about "Zinc metallothionein" patented technology

The invention belongs to the technical field of production and preparation of health bedding fabrics for teenagers, and relates to a far infrared anti-electromagnetic wave body-perfuming sleeping bag used for teenagers, comprising nano selenium and traditional Chinese medicinal materials and capable of generating negative ions. Oxygen is necessary for life, life activities can not continue without oxygen, and a brain will die if no oxygen gas is supplied for 5 minutes, while 20% of oxygen forms oxygen radicals which are harmful for a human body during metabolism. The metallothionein in nano selenium-containing bee products has the effect of strongly scavenging free radicals, the nano powder of medical stone or tourmaline has a far infrared anti-electromagnetic wave function, and the molecules of nano silver antimicrobial agent and traditional Chinese medicinal materials such as sweet osmanthus flower, jasmine flower and peach flower penetrate into deep skin tissues at a depth of 3-5 mm. The sleeping bag provided by the invention can be automatically heated, and has the effects of promoting metabolism, tissue vitality and blood circulation and perfuming skin. The spontaneously generated negative ions have the effects of regulating surrounding air quality, enhancing immunity, and strongly killing bacteria and mites.

The invention provides a multi-copy metallothionein recombinant expression vector and a method thereof for high-efficiency expression of metallothionein, and belongs to the technical field of biological chemistry. The method is as follows: constructing the multi-copy metallothionein recombinant expression vector; converting the constructed multi-copy metallothionein recombinant expression vector in pichia pastoris GS115 or KM71, and screening on a histidine-free culture medium to obtain recombinant bacteria; inoculating the screened recombinant bacteria in a BMGY culture solution, rapidly oscillating for culture, centrifugally collecting bacteria, resuspending with the culture solution, diluting, rapidly oscillating for culture until log phase, adding methanol, and feeding the methanol in batches to continue to cultivate; collecting the bacteria, ultrasonically crushing, collecting supernatant by centrifugation, and separating and purifying the supernatant to obtain the metallothionein. The method overcomes the defects of a traditional metallothionein preparation method, can effectively avoid the waste of a lot of animal resources and bacteria sourced endotoxin contamination, reduces the production cost, and improves the protein yield.

The invention discloses a ramie metallothionein gene and recombinant protein and an application thereof. The ramie metallothionein gene is a nucleotide sequence shown as the sequence table SEQ ID NO.1, and the ramie metallothionein is an amino acid sequence shown as the sequence table SEQ ID NO.2. A new metallothionein gene is obtained from ramie which is a herbaceous plant through in-vivo cloning, a recombinant expression engineering strain is constructed, and the recombinant metallothionein is obtained through expression and purification. The recombinant protein contains 12 cysteines (Cys),is abundant in mercapto groups that can chelate with cadmium and other heavy metal ions, and has wide application prospects in the fields of preparation of a heavy metal chelating antidote, chemical or medicine or heavy metal polluted cultivated land and pollution treatment of agricultural non-point sources.

The invention provides a fusion protein of a human epidermal growth factor and metallothionein, which comprises a first part and a second part, wherein the first part is the human epidermal growth factor, the second part is the human metallothionein, and the human metallothionein is positioned at the C terminal of the human epidermal growth factor. The invention further provides an application of the fusion protein in the preparation of drugs for treating burns or scalds or wounds or ulcers and the application of the fusion protein in the preparation of skin nursing drugs. Compared with the original human epidermal growth factor and the metallothionein, the fusion protein can increase the stability, reduce the clearance rate, reduce the degradation, bring benefits and convenience to the use of the EGF-MT fusion protein and further reduce the using dosage and the medication frequency. Simultaneously, the formed fusion protein is a bifunctional molecule which has double effects of EGF and MT.

The invention discloses Yunnan red pear-1 metallothionein gene PyMT1 and a prokaryotic expression vector thereof, and the prokaryotic expression vector is a prokaryotic expression vector which contains the Yunnan red pear-1 metallothionein gene PyMT1. According to the invention, PyMT1 gene is cloned from Yunnan red pear-1; the over expression of the gene in bacterial cells is controlled by T7 promoter and lactose operon with the induction of IPTG; PyMT1 protein is synthesized and secreted into cytoplasm; the expression level is detected by SDS-PAGE; test results show that the expression level of the PyMT1 protein reaches a maximum when the expression is inducted by 1mM IPTG at 37 DEG C for 4 hours and when the expression is inducted by 0.5 mM IPTG at 37 DEG C for 4 hours; the vector adopted by the invention can successfully express a lot of Yunnan red pear-1 metallothionein; the test results also demonstrates that the Yunnan red pear-1 metallothionein gene PyMT1 of Yunnan red pear has a typical function of heavy metal ion tolerance of the metallothionein gene, which lays a foundation for further research of PyMT1 functions and the applications of the metallothionein gene in microbial tolerance to heavy metal.

The invention discloses a method which is feasible in pharmaceutical industry for efficient secretion expressing, preparing and purifying of gene recombinant human metallothionein or analogues thereof. The method is characterized by introducing exogenous genes containing dual-promoters into relevant host yeast cells in transformation or double crossover recombination manner by using plasmid vector pPICZ alpha-MT of exogenous metal sulfur protein genes which contain methanol response element AOX and contain or not contain metal response element MRE to construction expression engineering cells containing the dual-promoters in the methanol yeast host cells. According to the invention, several problems with traditional preparation method of metallothionein, such as high production cost, slaughter and organ harvesting to large number of animals, virus pollution derived from animal, endotoxin pollution derived from bacterial, immunogen of non-humanized protein and the like, are avoided effectively,.

The invention discloses a gene engineering applying method of elsholtzia metal sulphur protein gene EhMT1 in Haizhou, wherein the GenBank entry number of cDNA sequence of EhMT1 is DQ059081 and the coded amino acid sequence is AAY57923; EhMT1 participates the accumulation of Haizhou elsholtzia root; the analysis of mRNA expression is that the Haizhou elsholtzia is induced by excessive copper; EhMT1 can be goal gene leading-in plant, which improves the copper resistance of plant and the accumulation of copper.

The invention relates to an industrial method for extracting and purifying zinc metallothionin from rabbit liver. It comprises following steps: inducing, coarse extracting, chromatography purifying; inducing adult rabbit for 5- 7 times through belly hypodermic injection, the zinc injection dosage is increased by 10- 250 mg for every kg rabbit gradually; stirring rabbit liver with homogenate liquor, adding denatured liqour and stirring, freezing and centrifuging, collecting supernatant -1, heating fastly, colling fastly to normal temperature, freezing and centrifuging again, getting supernatant -2, condensing through ultra- filtering membrane, getting supernatant -3 for chromatography in gel medium, desalting collecting liquid and freeze drying. The invention is characterized by higher productivity of Zn- MT, low extracting cost, high purity, simple preparation process and convenient extracting method.

The invention relates to a radiation-resistant skin cream composition, which comprises the following components in percentage by weight: 5.0% of aloe gel extractive, 3.0% of pyrrolidone, 5.0% of tea polyphenol, 1.0% of seabuckthorn seed oil, 8.0% of arbutin, 3.0% of hyaluronic acid, 1.0% of metallothionein, 0.5% of vitamin B3, 0.5% of vitamin E, 0.4% of vitamin C, 0.3% of vitamin A, and the balance of deionized water. The radiation-resistant skin cream composition disclosed by the invention can be used for effectively resisting ultraviolet light and skin injury by separable radiation and environmental pollution (waste gas, waste water and oily fume); and the radiation-resistant skin cream composition is particularly beneficial to caring skin of workers outdoors and in the front of a fluorescent screen, and has the effects of removing stain, preventing chromatosis, accelerating skin inflammation rehabilitation and relieving heavy metal toxicity and skin allergic reaction.

The invention relates to an African swine fever virus P22 protein nanoparticle and a preparation method and application thereof, the protein nanoparticle is formed by self-assembly of a protein monomer, and the protein monomer is a fusion protein sequentially containing metallothionein, glutathione thiotransferase and P22 protein from amino acid to a carboxyl terminal. The GSTP1-MT3 protein and the P22 antigen of the African swine fever virus are subjected to fusion expression, and in a pichia pastoris expression system, protein nanoparticles can be spontaneously formed through induction of ferrous ions. Immunogenicity determination is carried out in a mouse body, and the P22 protein nanoparticle can cause very strong immune response and has great potential to be developed into a new safeand effective African swine fever virus vaccine.

The invention discloses a yeast strain for displaying metallothionein on a cell surface and application of the same in heavy metal adsorption. The yeast strain for displaying metallothionein on the cell surface is obtained by integrating a box gene for displaying metallothionein on the cell surface and a constitutive promoter P<PGK1> gene into the chromosome of a host stain via an integrative vector; and the yeast strain can continuously and stably express metallothionein on the cell surface. The recombinant Saccharomyces cerevisiae strain provided by the invention has good adsorption capability and tolerance to a plurality of heavy metals like Cd<2+>, Cu<2+> and Cr<6+>; e.g., the strain needs 12 h for complete reduction of Cr<6+> with a concentration of 50 mg/L, 6 h faster compared with an original strain, and has a reduction rate 1.5 times of the reduction rate of the original strain; the removal rates of the strain to Cu<2+>, total Cr and Cd<2+> are increased by 13.35%, 10.13% and 19.39% respectively compared with the original strain; so the strain has great application potential in the field of treatment of heavy metals.

A composite medicine used also as the additive of cosmetics and health-care food for lowering blood viscosity, dissolving thrombus, and regulating blood pressure, blood sugar and blood fat is prepared through extracting to obtain L-polybasic metallothionein (L.pMT), lumbrikinase and antibacterial peptide, proportionally mixing it with the extracts of astragalus root and red sage root, thiooctanoic acid and 1,6-pectose-zinc biphosphate, and dispensing.

The present invention relates to neural cell survival, differentiation and proliferation promoting peptide fragments derived from metallothioneins (MT), pharmaceutical compositions comprising said peptide fragments and uses thereof for treatment of diseases and conditions where the effects of stimulating neural cell proliferation, differentiation and/or survival, and/or stimulating neural plasticity associated with learning and memory are beneficial for treatment.

The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel nucleotide sequence for a promoter for the gene encoding metallothionein. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant or plant cell with a nucleotide sequence operably linked to one of the promoters of the present invention.

The invention discloses a metallothionein detection method based on a carbon quantum dot-gold nanoparticle system. The preparation method comprises the following steps of: carrying out hydrothermal reaction on L-glutamic acid and 4-aminosalicylic acid to prepare green fluorescent carbon quantum dots; respectively detecting the fluorescence intensity F0 of a carbon quantum dot-gold nanoparticle standard solution and the fluorescence intensity F of a series metallothionein standard solution, establishing a linear relation between (F-F0)/F0 and the metallothionein concentration cMTs, detecting the fluorescence intensity of a to-be-detected sample solution, and substituting the fluorescence intensity into the linear relation to calculate the metallothionein concentration of the to-be-detectedsample solution. The method disclosed by the invention can be used for simply, conveniently, quickly and sensitively detecting metallothionein, and is suitable for detecting metallothionein in any aqueous solution system, especially urine.

The invention relates to a method for improving the metallothionein content of spirulina. The method comprises the following steps: (1), vaccinating the spirulina to a culture medium to ensure that the spirulina grows to a logarithmic growth phase, wherein the temperature is 26 to 38 DEG C, the pH value is 8.25 to 9, and the culturing time is 3 to 10 days; and (2), culturing the spirulina after the step (1) continuously, wherein the temperature is adjusted to be 25 to be 35 DEG C, the pH value is controlled to be 9 to 11, the zinc ionic concentration reaches 0.01 to 0.09 mol/L, and the culturing time is 7 to 14 days, so as to obtain the spirulina rich in metallothionein. Through the adoption of the method provided by the invention, the source of metallothionein is greatly enlarged to reduce the production cost of metallothionein; and as metallothionein is extracted from spirulina, the advantages are outstanding, the growth is fast, the output is large, the processing is easy, and the metallothionein content of the spirulina cell cultured according to the method provided by the invention is improved by more than 100 percent.

The invention relates to the field of nano medicine, and especially relates to a fluorescence imaging probe, a preparation method and applications thereof. The nano fluorescence imaging probe comprises a material having a metal-organic skeleton structure and heavy metal ions. The probe can enter lung cancer cells through endocytosis. The nano fluorescence imaging probe can stimulate different lung cancer cells to generate different contents of metallothionein, then the probe can combine with metallothionein, then the probe and metallothionein are discharged from the cells, and the amount of residual nano fluorescence imaging probe in cells will determine the intensity of cell fluorescence images, so according to the difference of intensity of cell fluorescence images, the lung squamous cell carcinoma and lung adenocarcinoma can be precisely differentiated.

The invention provides a chlorella-based metallothionein preparation method. The method comprises the steps: respectively feeding zinc and copper ions to a chlorella reactor for culturing; concentrating through a microfiltration membrane; breaking the wall of chlorella at the normal pressure; extracting chlorophyll; and extracting metallothionein. According to the preparation method, algae can be used as the source of metallothionein, so that the production cost of metallothionein is obviously decreased; metallothionein extracted through the chlorella has outstanding advantages, can grow fast, and is high in accumulation and easily processed; dry chlorella powder with metallothionein extracted out can be used for preparing a feed of the animal husbandry; chlorophyll in the chlorella is extracted through ethyl alcohol, so that the influence of chlorophyll on extraction and separation of metallothionein can be reduced, and the quality of metallothionein can be improved; and then zinc and copper are utilized for alternative stress induction for preparing metallothionein; and therefore, the productive rate of the obtained metallothionein is up to 10g/250kg, and the high-purity metallothionein can be obtained after purifying.

The invention discloses an application of D609 in preparation of a medicine for preventing and treating retinal injury diseases. The Chinese name of D609 is tricyclic decane-9-yl-dithiocarbonate potassium salt, and the D609 is an antiviral and antitumor small molecular compound, which belongs to a selective inhibitor of phosphatidylcholine specific phospholipase (PC-PLC). According to the invention, the wide and deep research is carried out, the unexpected discovery is realized for the first time, the expression of metallothionein is up-regulated by the D609; the oxidative damage of RPE cellscan be effectively inhibited; therefore, the compound can be used for preventing or treating retinal injury diseases caused by oxidative stress injury of retinal pigment epithelial cells, especially,the compound can be extremely effectively used for preventing or treating macular degeneration diseases of retina, has no obvious drug toxicity, can effectively control the occurrence and developmentof injury diseases of retina, and provides a new theoretical support for developing unknown biological activity of D609 and future clinical treatment effects.

The invention discloses an extraction method for chlorella vulgaris metallothioneins with whitening functions. According to the method, zinc chloride is adopted for stress culture of common marine chlorella vulgaris, the optimal induction condition of the metallothioneins can be obtained through investigation of different zinc stress concentrations and stress times, and the metallothionein products are obtained through the processes of extraction, separation and purification. Tyrosinase activity inhibiting tests prove that when the concentration of the chlorella vulgaris metallothioneins is 1.2 mg/ml, the activity of diphenolase can be reduced by 87 percent and monophenolase doesn't show activity within 2300 s, which shows that the chlorella vulgaris metallothioneins have favorable whitening functions and a wide application prospect in the fields of cosmetics and health products.

The invention provides a sunscreen skin-moistening lotion which comprises, by mass, 5-30 parts of 2, 2'-dihydroxy-4-methoxybenzophenone, 3-10 parts of 3-(4-methylbenzal) camphor, 2-20 parts of zinc oxide, 2-8 parts of stearic acid, 1-5 parts of essence, 40-80 parts of water, 5-10 parts of tea polyphenol, 4-10 parts of zinc metallothionein and 10-30 parts of vaseline. The sunscreen skin-moistening lotion can nourish and whiten skin and delay skin aging and is simple in preparation method, low in cost and suitable for general use.