African swine fever virus P22 protein nanoparticle and preparation method and application thereof

An African swine fever virus and nanoparticle technology, applied in the field of African swine fever virus P22 protein nanoparticles and their preparation, can solve the problems of inability to resist virulent attack, low protective effect, and no protective effect.
CN112574318AActive Publication Date: 2021-03-30非零和(北京)投资管理有限公司
3 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
非零和(北京)投资管理有限公司
Publication Date
2021-03-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to an African swine fever virus P22 protein nanoparticle and a preparation method and application thereof, the protein nanoparticle is formed by self-assembly of a protein monomer, and the protein monomer is a fusion protein sequentially containing metallothionein, glutathione thiotransferase and P22 protein from amino acid to a carboxyl terminal. The GSTP1-MT3 protein and the P22 antigen of the African swine fever virus are subjected to fusion expression, and in a pichia pastoris expression system, protein nanoparticles can be spontaneously formed through induction of ferrous ions. Immunogenicity determination is carried out in a mouse body, and the P22 protein nanoparticle can cause very strong immune response and has great potential to be developed into a new safeand effective African swine fever virus vaccine.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an African swine fever virus P22 protein nanoparticle and a preparation method and application thereof. Background technique

[0002] African swine fever (African swine fever, ASF) is an acute, hemorrhagic, highly contagious infectious disease caused by African swine fever virus (ASFV) infecting domestic pigs or wild boars, characterized by short course of disease, high fever and Hemorrhagic lesions, acute infection mortality rate up to 100%, a serious threat to the global pig industry.

[0003] In the 1960s, people first tried to use inactivated ASF vaccines, but most of them had no protective effect. In 2014, Blome et al. used the latest adjuvant combined with ASF inactivated vaccine for immunization, but still could not resist the virulent attack. Using subunit vaccines prepared by protective antigens (p72, p54, CD2v, etc.), immunized pigs can only provide partial protection, bu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More