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Plants having increased yield-related traits and a method for making the same

A plant, yield technology, applied in the field of molecular biology, can solve the problems of average yield decline, crop loss, etc.

Inactive Publication Date: 2012-12-19
BASF PLANT SCI GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Abiotic stress is the leading cause of crop loss worldwide, reducing the average yield of most major crop plants by more than 50% (Wang et al., Planta (2003) 218:1-14)

Method used

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  • Plants having increased yield-related traits and a method for making the same
  • Plants having increased yield-related traits and a method for making the same
  • Plants having increased yield-related traits and a method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0519] Example 1: Identification of sequences related to nucleic acid sequences used in the methods of the invention

[0520] Using database sequence search tools such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215:403-410; and Altschul et al. (1997) Nucleic Acids Res. 25:3389-3402), Sequences (full-length cDNA, EST or genomic sequences) related to the nucleic acid sequences used in the methods of the invention were identified in the sequences maintained by the National Center for Biotechnology Information (NCBI) Entrez nucleotide database. The BLAST program is used to find regions of local similarity between sequences by comparing nucleic acid sequences or polypeptide sequences to sequence databases and by calculating the statistical significance of the matches. For example, the TBLASTN algorithm is applied to the polypeptide encoded by the nucleic acid sequence of the present invention, with the default setting and the filter turned on to...

Embodiment 2

[0526] Embodiment 2: Alignment of polypeptide sequences of the present invention

[0527] Polypeptide alignment was performed using the Vector NTI package (Invitrogen) AlignX program based on the popular progressive alignment ClustalW algorithm (Thompson et al (1997) Nucleic Acids Res 25:4876-4882; Chenna et al (2003) Nucleic Acids Res 31:3497-3500). Alignment of sequences. The default values ​​are gap opening penalty of 10, gap extension penalty of 0.1, and the selected weight matrix is ​​Blosum62 (if comparing peptides). Minor artificial edits can be made to further refine the alignment. A phylogenetic tree of polypeptides was constructed using the neighbor-joining clustering algorithm provided in the AlignX program of Vector NTI (Invitrogen).

[0528] Use progressive comparison Clustal algorithm (1.83), utilize default value (Thompson et al., (1997) Nucleic Acids Res25:4876-4882; Chenna et al., (2003).Nucleic Acids Res31:3497-3500), to Fujimoto etc. (2004 ; All Arabidops...

Embodiment 3

[0535] Example 3: Calculation of Global Identity Percentage Between Polypeptide Sequences That Can Be Used to Implement the Methods of the Invention

[0536] The global similarity and identity percentages between the full-length polypeptide sequences that can be used to implement the method of the present invention utilize one of the methods available in the art, i.e. MatGAT (Matrix Global Alignment Tool) software (BMC Bioinformatics.20034:29.MatGAT: An application that generates similarity / identity matrices using protein or DNA sequences. Campanella JJ, Bitincka L, Smalley J; software hosted by Ledion Bitincka), was identified. MatGAT software can generate similarity / identity matrices of DNA or protein sequences without pre-aligning the data. The program performs a series of pairwise alignments using the Myers and Miller global alignment algorithm (with a gap opening penalty of 12 and a gap extension penalty of 2) to calculate similarity and identity using, for example, Blosu...

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Abstract

The present invention relates generally to the field of molecular biology and concerns a method for increasing various plant yield-related traits by modulating expression in a plant of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19 / 20 (AHL19 / 20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide. The present invention also concerns plants having modulating expression of a nucleic acid sequence encoding a yield increasing polypeptide selected from the group consisting of: an AT-hook motif nuclear localized 19 / 20 (AHL19 / 20), a GRP (Growth Regulating Protein) (wherein said GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and an alanine aminotransferase (AAT) polypeptide which plants have increased yield-related traits relative to control plants. The invention also provides constructs useful in the methods of the invention.

Description

[0001] This application is a divisional application of the Chinese patent application 200880025418.0 (PCT / EP2008 / 059515) with the filing date of July 21, 2008, and the title of the invention is "Plant with Enhanced Yield-related Traits and Preparation Method thereof". field of invention [0002] The present invention generally relates to the field of molecular biology and relates to a method for enhancing various plant yield-related traits by increasing expression in a plant of a nucleic acid sequence encoding a yield-enhancing polypeptide selected from the group consisting of: [0003] Nuclear localized AT-hook motif protein 19 / 20 (AHL19 / 20), [0004] GRP (growth regulatory protein, wherein the GRP polypeptide is a metallothionein 2a (MT2a) polypeptide), an alanine aminotransferase (AAT)-like polypeptide, and [0005] Alanine aminotransferase (AAT) polypeptide. The present invention also relates to plants having increased expression of a nucleic acid sequence encoding said y...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/84A01H5/00A01H5/10C12N5/10C12Q1/68
CPCC12N15/8261Y02A40/146C12N15/8273
Inventor Y·海茨费尔德V·弗兰卡德
Owner BASF PLANT SCI GMBH
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