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Method for improving metallothionein content of spirulina

A technology of metallothionein and spirulina, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high technical difficulty and high production cost, and achieve a large accumulation, easy processing, and rapid growth Effect

Active Publication Date: 2012-06-27
何忠志
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the above-mentioned problem of high production cost and high technical difficulty, the present invention has carried out a lot of research on how to increase the content of metallothionein in spirulina. Method for sulfur protein content

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1) Spirulina was inoculated into Z's medium at an inoculation concentration of 10 ml / L, and cultured at 26°C and pH=8.25 for 7 days to reach the logarithmic growth phase.

[0026] 2) Continue culturing the spirulina that reached the logarithmic growth phase in step 1), adjust the temperature to 26°C, control the pH at 9, add zinc gluconate to make the zinc ion concentration reach 0.01mol / L, and cultivate for 7 days to obtain rich Spirulina containing metallothionein. The content of Spirulina metallothionein is 11.6g / 100g.

Embodiment 2

[0028] 1) Spirulina was inoculated into Z's medium at an inoculation concentration of 10 ml / L, and cultured at 38° C. and pH=9 for 10 days to reach the logarithmic growth phase.

[0029] 2) Continue culturing the spirulina that has reached the logarithmic growth phase in step 1), adjust the temperature to 35°C, control the pH at 11, add zinc chloride, the zinc ion concentration reaches 0.09mol / L, and cultivate for 14 days to obtain metal sulfur Spirulina with increased protein content. The content of Spirulina metallothionein is 9.0g / 100g.

Embodiment 3

[0031] 1) Spirulina was inoculated into Z's medium at an inoculation concentration of 10 ml / L, and cultured at 32°C and pH=8.8 for 3 days to reach the logarithmic growth phase.

[0032] 2) Continue culturing the spirulina that has reached the logarithmic growth phase in step 1), adjust the temperature to 30°C, control the pH at 11, add zinc sulfate, the zinc ion concentration reaches 0.07mol / L, and cultivate for 10 days to obtain metallothionein Increased content of spirulina. The content of Spirulina metallothionein is 14.4g / 100g.

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Abstract

The invention relates to a method for improving the metallothionein content of spirulina. The method comprises the following steps: (1), vaccinating the spirulina to a culture medium to ensure that the spirulina grows to a logarithmic growth phase, wherein the temperature is 26 to 38 DEG C, the pH value is 8.25 to 9, and the culturing time is 3 to 10 days; and (2), culturing the spirulina after the step (1) continuously, wherein the temperature is adjusted to be 25 to be 35 DEG C, the pH value is controlled to be 9 to 11, the zinc ionic concentration reaches 0.01 to 0.09 mol / L, and the culturing time is 7 to 14 days, so as to obtain the spirulina rich in metallothionein. Through the adoption of the method provided by the invention, the source of metallothionein is greatly enlarged to reduce the production cost of metallothionein; and as metallothionein is extracted from spirulina, the advantages are outstanding, the growth is fast, the output is large, the processing is easy, and the metallothionein content of the spirulina cell cultured according to the method provided by the invention is improved by more than 100 percent.

Description

technical field [0001] The invention relates to the field of biomass accumulation and extraction, in particular to a method for increasing the content of metallothionein in spirulina. Background technique [0002] Metallothionein (MT) is a kind of protein with low molecular weight, high sulfhydryl content, and unique ability to bind metal ions. Studies have proved that MT can widely exist in various organisms, including various microorganisms, various tissues and organs of higher animals, plants and humans. After being cultured under appropriate culture conditions and culture medium, the metallothionein can be separated and extracted by chromatography. [0003] The metallothionein molecule is elliptical, with an average molecular mass of 6500u, divided into two structural domains, each molecule contains 7 to 12 metal atoms, and has special light absorption. Studies have found that metallothionein has strong antioxidant activity, and its ability to scavenge superoxide anion...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12R1/89
Inventor 常蓉李博生
Owner 何忠志
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