Chlorella-based metallothionein preparation method
A metallothionein and chlorella technology, which is applied in the preparation methods of metallothionein and peptides, and methods based on microorganisms, etc., can solve the problems of high transfer fee, large equipment investment, low output, etc., to improve quality and reduce Production cost, rapid growth effect
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Embodiment 1
[0027] Chlorella-based metallothionein preparation method, according to the following steps:
[0028] Step 1, the raw material of chlorella culture fluid is the NaHCO of 2g / L 3 , 0.5g / L of KNO 3 , 0.02g / L of KH 2 PO 4 , 0.3mg / L of V B1 and a V of 1.0μg / L B12 , put the culture solution in the reactor, sterilize at 120°C, inoculate the chlorella and the culture solution at a ratio of 1:3 by volume, then place it in a light incubator at 25°C, cultivate it for 12 hours, and then put the reaction Place the device in an incubator at 25°C without light, incubate for 5 hours, and then put it in an incubator with light for 12 hours, repeat this 10 times, and add 5 μmol / L zinc sulfate to the chlorella in the logarithmic growth phase Solution, after culturing for 120 hours, add 5 μmol / L copper chloride solution to the chlorella after the above-mentioned treatment, and then cultivate for 10 days;
[0029] Step 2, after culturing for 10 days, concentrate the culture solution and the ...
Embodiment 2
[0035] Chlorella-based metallothionein preparation method, according to the following steps:
[0036] Step 1, the raw material of chlorella culture fluid is the NaHCO of 2g / L 3 , 0.5g / L of KNO 3 , 0.02g / L of KH 2 PO 4 , 0.3mg / L of V B1 and a V of 1.0μg / L B12 , put the culture solution in the reactor, sterilize at 120°C, inoculate the chlorella and the culture solution at a ratio of 1:1 by volume, then place it in a light incubator at 25°C, cultivate it for 12 hours, and then put the reaction Place the device in an incubator without light at 25°C for 5 hours, and then put it in an incubator with light for 12 hours. Repeat this three times. Add 100 μmol / L chloride to the chlorella in the logarithmic growth phase. Zinc and zinc gluconate mixed solution, after cultivating for 24 hours, then add 100 μmol / L copper sulfate and copper gluconate mixed solution to the chlorella after the above treatment, and then cultivate for 3 days;
[0037] Step 2. After 3 days of cultivation, ...
Embodiment 3
[0043] Chlorella-based metallothionein preparation method, according to the following steps:
[0044] Step 1, the raw material of chlorella culture fluid is the NaHCO of 2g / L 3 , 0.5g / L of KNO 3 , 0.02g / L of KH 2 PO 4 , 0.3mg / L of V B1 and a V of 1.0μg / L B12 , put the culture solution in the reactor, sterilize it at 120°C, inoculate the chlorella and the culture solution according to the volume ratio of 1:2, then put it in the light incubator at 25°C, cultivate it for 12h, and then put the reaction Place the device in an incubator without light at 25°C for 5 hours, and then put it in an incubator with light for 12 hours. Repeat this 8 times. Add 50 μmol / L chloride to the chlorella in the logarithmic growth phase. Zinc solution, after cultivating for 90 hours, add 70 μmol / L copper chloride solution to the chlorella after the above treatment, and then cultivate for 8 days;
[0045] Step 2, after culturing for 8 days, concentrate the culture solution and the chlorella in it...
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