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Switching detection method for eluent

A detection method and eluent technology, applied in the field of elution, can solve problems such as inaccurate switching timing of eluent, and achieve the effect of increasing the content

Active Publication Date: 2020-08-18
CHANGDE ZHENGYANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a detection method for switching eluents, aiming to solve the problem of inaccurate switching timing of the existing eluents

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] An embodiment of the present invention provides an eluent switching detection method, including:

[0032] Prepare a silica gel plate with a size of 2cm*6cm, specify a starting line parallel to one side of the silica gel plate, specify three points on the starting line, and the distance between the starting line and one side of the silica gel plate is 7mm;

[0033] When judging whether the eluent contains solanesol, prepare the solanesol standard sample, point the standard sample to the middle point of the three points, and the distance between two adjacent points is 5mm;

[0034] Take the sample to be detected, that is, the eluent containing solanesol, and spot the sample to be detected on the other two points;

[0035] Put the silica gel plate obliquely into the developing slot, add the developing solution to the developing slot to a depth of 5 mm, make the developing solution flush with the starting line, expand the standard sample and the sample to be tested to a pos...

Embodiment 2

[0039] The difference between this embodiment and the first embodiment is that the developing solution includes 85% of n-hexane and 15% of ethyl acetate by weight percentage, and the others are the same as the first embodiment.

Embodiment 3

[0041] The difference between this embodiment and the first embodiment is that the developing solution includes 95% n-hexane and 5% ethyl acetate by weight percentage, and the other is the same as the first embodiment.

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Abstract

The method is suitable for the technical field of elution. The invention provides a switching detection method of an eluent. The method comprises the steps: employing a point plate analysis method, selecting n-hexane and ethyl acetate as developing solutions of solanesol and phenolic substances, performing reasonable blending respectively, and accurately detecting solanesol and phenolic substancesin the eluent; judging the switching time of the eluent more accurately by combining conventional theoretical calculation, so the content of effective components in the eluent obtained in each elution stage is increased, and finally, the yield of tobacco leaf essential oil, solanesol and phenolic substances in tobacco leaves is increased.

Description

technical field [0001] The invention belongs to the technical field of elution, and in particular relates to a detection method for switching an eluent. Background technique [0002] Elution refers to the process in which the mobile phase continuously passes through the stationary phase of the chromatographic column, and the components of the sample are washed out from the stationary phase in sequence. Generally, the force between the mobile phase and the stationary phase is weaker than that of the sample. Most chromatographic separations use elution. The elution method can be divided into isocratic elution and gradient elution. [0003] The process of preparing active ingredients such as tobacco leaf essential oil, solanesol, and polyphenols from tobacco leaves includes: extracting tobacco leaves with an organic solvent to obtain an extract, concentrating the extract, and eluting and separating the extract. The eluent will elute the various effective components in the conc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 曾勇黄平易松山杨鸿波陈德亮陈孟军
Owner CHANGDE ZHENGYANG BIOTECH CO LTD