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Method for improving seed germination and/or plant tolerance to environmental stress

A technology for tolerance, plants, applied in the field of producing them, seedlings or plants or parts thereof, capable of solving the problems of loss of effectiveness of coating treatments, erosion, loss of components or active ingredients, etc.

Active Publication Date: 2020-08-18
DANSTAR FERMENT AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Depending on the composition of the seed coating, components or active ingredients are prone to loss or may be attacked by biological activity in the field (such as microorganisms or pests), which will lead to an eventual loss of the effectiveness of the coating treatment

Method used

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  • Method for improving seed germination and/or plant tolerance to environmental stress
  • Method for improving seed germination and/or plant tolerance to environmental stress
  • Method for improving seed germination and/or plant tolerance to environmental stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1: Effect of External / Foliar Application of Glycine-Betaine on Internal Concentration of Glycine-Betaine in Maize Seeds

[0067] method:

[0068] Field trials were conducted with two different maize varieties: LG 32.58 variety and Casey variety.

[0069] To prevent self-fertilization during maize seed production, the male reproductive parts of the female lines are removed before the time of pollen shedding.

[0070] At the stage of grain development, it will be sold commercially at an equivalent of 4kg / ha (Lallemand) formulation sprayed to female line leaves. Each replicate consisted of 18 plants. Control plants were treated with water.

[0071] Collect seeds (200) at maturity. The seeds were ground using a ball mill and extracted with ethanol. Samples were evaporated to dryness and dissolved in deuterium oxide (D2O) (50 mg / ml). Using 3-(trimethylsilyl)-1-propanesulfonic acid sodium salt (DSS) as internal standard 1 H-NMR analysis (Bruker, at 500 MHz). ...

Embodiment 2

[0086] Example 2: Effect of cold temperature on corn seed germination

[0087] method:

[0088] Two hundred seeds of each treatment (plants treated with water and plants treated with glycine-betaine) were planted on moistened sand. The samples were placed in a germination chamber at 10°C for 7 days. The samples were then transferred to a germination chamber set at 25°C for 5 days. Experiment with a variety of seeds that have increased hardiness compared to more traditional seed varieties. Standard seeds germinate 50-70% after the cold germination test.

[0089] result:

[0090] Table 5: Effect of cold temperature on maize seed germination

[0091]

[0092] S - means statistically significant difference

[0093] The results disclosed in Table 5 show that seeds with increased internal content of glycine-betaine after cold treatment have a significant Better seed germination percentage.

[0094] ***

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PUM

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Abstract

The present disclosure relates to a seed of a plant, a seedling or a plant derived from the seed containing an increased internal concentration of glycine-betaine compared to untreated controls. The present disclosure further relates to a method to produce a seed of a plant, a seedling or a plant derived from said seed containing an increased internal concentration of glycine-betaine which will confer to the seed, seedling or plant derived from said seed an enhanced germination potential and / or increased tolerance against various environmental stresses compared to untreated controls. The present disclosure further relates to uses of glycine-betaine for enhancing the germination potential of a seed and / or for increasing the tolerance of a seed against environmental stress. The present disclosure also relates to seedlings and plants, or parts thereof, derived from a seed of the present invention having an increased international concentration of glycine-betaine. The present disclosure further relates to seedlings or plants, or parts thereof, obtainable by the methods of the present invention.

Description

technical field [0001] The present disclosure relates to seeds of plants containing increased internal concentrations of glycine-betaine, seedlings or plants derived from said seeds, and methods for producing the same. The present disclosure also relates to a method for providing a benefit to a plant seed or a seedling or plant derived from said seed. More particularly, the present disclosure provides a method for producing or derived from plant seeds having enhanced seed germination and / or increased tolerance to various environmental stresses (environmental stress) seedlings or plants. The present disclosure also relates to the use of glycine-betaine for enhancing the germination potential of seeds and / or for increasing the tolerance of seeds to environmental stress. The present disclosure also relates to seeds, seedlings and plants or parts thereof derived from the invention having an increased international concentration of glycine-betaine. The present disclosure also re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/44A01P21/00
CPCA01N37/44
Inventor 奥利维尔·科尔
Owner DANSTAR FERMENT AG
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