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Application of 5-iodo-tuberculocin in preparation of antifungal drugs

A technology of tuberculin and antifungal drugs, applied in the field of medicine, can solve the problems of less research in the field of antifungal infections

Active Publication Date: 2020-08-25
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 5-Itu has also been reported in the field of anti-bacterial infection in recent years, but there is less research in the field of anti-fungal infection
At present, there is no report on the antifungal effect of 5-Itu at home and abroad

Method used

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  • Application of 5-iodo-tuberculocin in preparation of antifungal drugs
  • Application of 5-iodo-tuberculocin in preparation of antifungal drugs
  • Application of 5-iodo-tuberculocin in preparation of antifungal drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The effect of embodiment 1,5-Itu on different fungi

[0016] Materials and methods

[0017] 1. Test drug:

[0018] 5-Itu (CAS No. 24386-93-4): purchased from Shanghai Kairui Biotechnology Co., Ltd., purity ≥ 99%.

[0019] Fluconazole: sigma company, batch number 036M4709V.

[0020] Dimethylsulfoxide (DMSO): Tianjin Tianli Chemical Reagent Co., Ltd.

[0021] Each reagent was prepared in DMSO and stored at -20°C. Before the experiment, the drug was taken out and placed in a 35°C incubator to melt, and mixed thoroughly.

[0022] 2. Strains:

[0023] Strains Used: Including:

[0024] 1. Sensitive strains (sensitive to fluconazole): sensitive Candida albicans (SC5314), Candida parapsilosis (22019), Cryptococcus neoformans (32609);

[0025] 2. Drug-resistant strains (resistant to fluconazole): drug-resistant Candida albicans (103), drug-resistant Candida tropicalis, naturally drug-resistant Candida krusei (ATCC2340), naturally drug-resistant Candida glabrata (ATCC2281)...

Embodiment 2

[0052] Embodiment 2, the impact of 5-Itu on the survival of different fungi

[0053] experimental method:

[0054] 1, medicine, bacterial strain, material are with embodiment 1

[0055] 2. Experimental steps:

[0056] Before the experiment, a small number of bacterial clones were picked from the SDA medium stored at 4°C, inoculated into 1mL YEPD medium, and cultured at 35°C, 200rpm shaking for 16h, so that the fungus was in the late exponential growth phase. Dilute with RPMI1640 liquid culture medium and adjust the bacterial concentration to 1×10 5CFU / mL. Add fluconazole and compound 5-Itu to the above bacterial liquid respectively, so that the final concentration of fluconazole is 1 μg / mL, the final concentration of compound 5-Itu is 8 μg / mL, 4 μg / mL and 2 μg / mL, and the blank control is added with the same volume of DMSO, the content of DMSO in all bacterial solutions was lower than 0.5%. In a 35°C incubator, shake culture at 200rpm, take samples at 0h, 6h, 12h, 24h, an...

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Abstract

The invention provides application of 5-Itu in preparation of antifungal drugs. In-vitro cell experiments show that 5-Itu has broad-spectrum antifungal activity and has different degrees of antifungaland even fungicidal effects on drug-resistant candida albicans 103, sensitive candida albicans SC5314, cryptococcus neoformans 32609, candida krusei ATCC2340, candida parapsilosis 22019, candida glabrata ATCC2281 and drug-resistant candida tropicalis. Therefore, 5-Itu can be used as an antifungal drug and used for preparing antifungal drugs, and a new candidate drug is provided for fungal infection treatment; and 5-Itu has the characteristics of broad antifungal spectrum and fungicidal activity, and a new treatment way is provided for clinical efficient treatment of fungal and even drug-resistant fungal infection.

Description

technical field [0001] The invention belongs to the technical field of medicine, and specifically relates to the application of 5-iodo-tubercidin in the preparation of antifungal drugs. Background technique [0002] 5-Iodotubercidin (5-Idotubercidin, abbreviated as 5-Itu) is a highly efficient adenosine kinase inhibitor. Convulsions, melanin removal, regulation of fatty acid metabolism and other functions. [0003] 5-Itu has also been reported in the field of anti-bacterial infection in recent years, but there is less research in the field of anti-fungal infection. At present, there is no report on the antifungal effect of 5-Itu at home and abroad. Contents of the invention [0004] Aiming at the problems existing in the prior art, the present invention provides the use of 5-Itu for preparing antifungal drugs. [0005] The present invention is achieved through the following technical solutions: [0006] Use of 5-iodo-tubercidin for the preparation of antifungal drugs. ...

Claims

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Application Information

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IPC IPC(8): A61K31/7064A61P31/10
CPCA61K31/7064A61P31/10
Inventor 刘伟王苗苗倪萍韩研春温芝潼
Owner SHAANXI UNIV OF SCI & TECH