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Experimental method for promoting neuron function by using small-molecule sea cucumber peptide

An experimental method, sea cucumber peptide technology, applied in the field of functional testing of active peptides, can solve problems such as no method for active peptides to promote neuron function testing, and achieve simple and effective operations

Pending Publication Date: 2020-08-25
好当家集团有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many active peptides in the market. However, there is no way to accurately test the neuron-promoting function of these active peptides. How to test the neuron-promoting function of small molecule sea cucumber peptides has become an urgent problem to be solved.

Method used

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  • Experimental method for promoting neuron function by using small-molecule sea cucumber peptide
  • Experimental method for promoting neuron function by using small-molecule sea cucumber peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] An experimental method for promoting neuron function by small-molecule sea cucumber peptides, comprising the following steps:

[0016] a. Prepare a small molecule sea cucumber peptide solution with a mass concentration of 6%. Specifically, put the small molecule sea cucumber peptide powder into water at 72°C to fully dissolve and stir evenly. The dissolution time is controlled for 3.5 minutes;

[0017] b. The small molecule sea cucumber peptide solution is used for neuron-promoting function test, specifically: select pregnant test mice, take out the embryo, remove the outer cell membrane of the embryo, remove the head, limbs and internal organs, and transfer the remaining embryos to a petri dish filled with PBS Rinse in medium, take out the C1-C3 area of ​​the spine under a biodissecting microscope, finely chop it with sterilized scissors, transfer it together with PBS solution to a 15ml centrifuge tube, centrifuge at 1000 rpm for 5 min, pour off the supernatant, and add...

Embodiment 2

[0020] An experimental method for promoting neuron function by small-molecule sea cucumber peptides, comprising the following steps:

[0021] a. Prepare a small molecule sea cucumber peptide solution with a mass concentration of 3%, specifically, put the small molecule sea cucumber peptide powder into water at 70°C to fully dissolve and stir evenly, and the dissolution time is controlled for 2.5 minutes;

[0022] b. The small molecule sea cucumber peptide solution is used for neuron-promoting function test, specifically: select pregnant test mice, take out the embryo, remove the outer cell membrane of the embryo, remove the head, limbs and internal organs, and transfer the remaining embryos to a petri dish filled with PBS Rinse in medium, take out the C1-C3 area of ​​the spine under a biodissecting microscope, finely chop it with sterilized scissors, transfer it together with PBS solution to a 15ml centrifuge tube, centrifuge at 1000 rpm for 5 min, pour off the supernatant, and...

Embodiment 3

[0025] An experimental method for promoting neuron function by small-molecule sea cucumber peptides, comprising the following steps:

[0026] a. Prepare a small molecule sea cucumber peptide solution with a mass concentration of 8%, specifically, put the small molecule sea cucumber peptide powder into water at 72°C to fully dissolve and stir evenly, and the dissolution time is controlled for 5 minutes;

[0027] b. The small molecule sea cucumber peptide solution is used for neuron-promoting function test, specifically: select pregnant test mice, take out the embryo, remove the outer cell membrane of the embryo, remove the head, limbs and internal organs, and transfer the remaining embryos to a petri dish filled with PBS Rinse in medium, take out the C1-C3 area of ​​the spine under a biodissecting microscope, finely chop it with sterilized scissors, transfer it together with PBS solution to a 15ml centrifuge tube, centrifuge at 1000 rpm for 5 min, pour off the supernatant, and a...

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Abstract

The invention discloses an experimental method for promoting a neuron function by using small-molecule sea cucumber peptide. The experimental method comprises the following steps of a, preparing an equal amount of small-molecule sea cucumber peptide solution; b, testing the neuron promoting function of the small-molecule sea cucumber peptide; and c, carrying out observation of neuronal cell changes. The experimental method for promoting the neuron growth function by using the small-molecule sea cucumber peptide is simple and effective, and can be used as a detection method for identifying thequality of the active peptide.

Description

technical field [0001] The invention relates to the technical field of functional testing of active polypeptides, in particular to an experimental method for promoting neuron function by small molecule sea cucumber peptides. Background technique [0002] Sea cucumbers are distributed in all seas of the world, and the most species are in the Indo-West Pacific region, especially edible sea cucumbers, which are mostly distributed in tropical coral reefs. There is only one kind of edible sea cucumber in northern my country, that is sea cucumber, while Hainan Island and Paracel Islands produce more than a dozen edible sea cucumbers. Edible sea cucumbers mostly live in the intertidal zone or shallow sea. Sea cucumbers are distributed in the deep sea and even in the abyss. Most of them are flat-footed sea cucumbers. Sea cucumbers have strong regenerative power. When stimulated or in an unfavorable environment, such as dirty water and lack of oxygen, the body often contracts strong...

Claims

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Application Information

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IPC IPC(8): C12N5/0793G01N33/569
CPCC12N5/0619G01N33/56966C12N2509/00C12N2501/998C12N2501/13
Inventor 孙永军鞠文明鞠晓庆毕囡囡邱美茹王鹏程彭莉晶唐晓波吴学记刘炳岗
Owner 好当家集团有限公司
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