A method of using single nucleotide polymorphism (SNP) markers to identify individual animals
A polynucleotide, individual technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve polymorphism microsatellite data difficult to distinguish a certain relationship, low mutation rate, etc. question
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[0123] Tag screening
[0124] The dog genome information was downloaded from the National Human Genome Research Institute Dog Genome (NHGRI Dog Genome) project released in 2017 and 2018, consisting of about 1,355 dog genome data in 2017 and 722 dog genome data in 2018. Approximately 127 dog genome data were downloaded from iDOG (DOGSD). The reference genome was used for CanFam 3.1.199, and the dog breed information was collected from Dogbreedchart ( http: / / www.dogbreedchart.com / ). Common names were extracted from the NHGRI Dog Genome Project and Dogbreedchart. The relevant information of each pedigree is deduced from the SNP of each pedigree, and because the size of the currently available specimens of each breed is relatively small, it is possible to avoid inbreeding during the entire marker selection process. Then, cultivars with a cohort size of less than 10 were removed, leaving only ~66 cultivars. As a result, candidate markers were screened on the basis of cultivar...
Embodiment 1
[0170] Embodiment 1: candidate marker screening
[0171] Such as figure 1 Candidate markers were screened as shown, at which point different dog samples were identified, as figure 2 shown. A total of 18 dog samples including 6 cell lines were collected. Candidate markers were tested in samples from 18 dogs, including cell lines from 6 dogs, and 100%, 97.22% and 89% coverage were confirmed for 14 samples, 3 samples and 1 sample, respectively ( table 3).
[0172] Likewise, 100%, 96% and 92% of cholate were confirmed for 12 specimens, 5 specimens and 1 specimen respectively (Table 4)
[0173] Gene sequence number 20 was analyzed, as 4 non-detections were shown in 36 samples (18 sample repeats) in "37_18379068", 0 to 1 non-detection proved that all 25 identifications were good (Table 3). The characteristic information of the dog samples used in the experiment is shown in Table 2 above. Intraoral swab samples from approximately 12 dogs and cell line samples from 6 dogs we...
Embodiment 2
[0180] Embodiment 2: reproducibility verification
[0181] To verify reproducibility, 18 samples were replicated and 2 different experimental plates were prepared at different times. Table 5 shows the cholate esters of the samples. The results are shown in Table 5, and it was verified that the marker according to the present invention exhibited high reproducibility.
[0182] table 5
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