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Application of SOX17 protein in preparation of renal clear cell carcinoma diagnosis or prognosis evaluation kit

A technology for clear cell renal cell carcinoma and SOX17, which is applied in the preparation of diagnostic or prognosis assessment kits for clear cell renal cell carcinoma, and can solve the problems of unclear molecular mechanism of SOX17 and no SOX17 protein.

Inactive Publication Date: 2020-09-08
SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no research report on SOX17 in clear cell renal cell carcinoma, and the molecular mechanism of SOX17 in the development of clear cell renal cell carcinoma is still unclear.
And there is no research report on the application of SOX17 protein in the preparation of renal clear cell carcinoma diagnosis or the prediction of the prognosis of patients with renal clear cell carcinoma

Method used

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  • Application of SOX17 protein in preparation of renal clear cell carcinoma diagnosis or prognosis evaluation kit
  • Application of SOX17 protein in preparation of renal clear cell carcinoma diagnosis or prognosis evaluation kit
  • Application of SOX17 protein in preparation of renal clear cell carcinoma diagnosis or prognosis evaluation kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] A total of 458 patients with clear cell renal cell carcinoma were selected from two centers for paraffin sections (343 cases of clear cell renal cell carcinoma were obtained from Shanghai Changhai Hospital and 115 cases from Shanghai Cancer Center of Fudan University in two centers, and were diagnosed by 2 pathologists. clear cell renal cell carcinoma), the immunohistochemical method was used to detect the expression of SOX17 protein in the cancer tissues and adjacent normal tissues of clear cell renal cell carcinoma patients, and the immunohistochemical score was calculated. The specific steps were as follows:

[0058] (1) Paraffin sections of clear cell renal cell carcinoma tissue were prepared, and the oven was set at 60°C overnight;

[0059] (2) slices are dewaxed to water;

[0060] (XyleneⅠ①10min→XyleneⅡ②10min→XyleneⅢ③10min→100% ethanol 5min→95% ethanol 5min→85% ethanol 5min→75% ethanol 5min→double distilled water 5min)

[0061] (3) 3%H 2 o 2 Solution, placed at...

Embodiment 2

[0080] Combined with the prognostic information, 458 patients with clear cell renal cell carcinoma merged from the two centers in Example 1 were divided into Center 1 (343 cases from Shanghai Changhai Hospital) and Center 2 (115 cases from Shanghai Cancer Center of Fudan University). Using the H-score score of SOX17 protein in 343 patients in Center 1, the Receiver Operating Characteristic Curve (Receiver Operating Characteristic Curve, ROC) analysis was performed according to the 5-year survival time as the standard, and the data analysis was performed using R software (version 3.5.3). The best cut-off value cut off is 100 (see figure 2 ).

Embodiment 3

[0082] Combined with the prognosis information, the cut off values ​​obtained in Example 2 were used to divide 343 cases in Center 1, 115 cases in Center 2, and 458 patients from the merger of the two centers into the SOX17 high expression group (H-score greater than or equal to 100) and the SOX17 The low expression group (H-score less than 100) was statistically analyzed for pathological features, and data analysis was performed using SPSS software (version 17.0). The results showed that the SOX17 high expression group had lower TNM stage, WHO / ISUP nuclear grade and SSIGN score (see Table 1-3).

[0083] Table 1 Correlation between SOX17 expression and clinicopathological features in patients with clear cell renal cell carcinoma in Center 1

[0084]

[0085]

[0086] Table 2 Correlation between SOX17 expression and clinicopathological features in patients with clear cell renal cell carcinoma in center 2

[0087]

[0088] Table 3 Correlation between SOX17 expression a...

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Abstract

The invention relates to the technical field of biology, in particular to application of SOX17 protein in preparation of a renal clear cell carcinoma diagnosis or prognosis evaluation kit. A brand-newbiomarker is provided for diagnosis or prognosis prediction of the renal clear cell carcinoma by discovering the correlation between the SOX17 protein and the renal clear cell carcinoma. Expression of the SOX17 protein in the tumor tissue of a patient with renal clear cell carcinoma is remarkably reduced, the reduction amplitude is related to the survival time of the patient, and the low expression is remarkably related to the prognosis difference of the patient, so that the kit has great guiding significance for diagnosis and treatment of the patient with renal clear cell carcinoma.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of SOX17 protein in the preparation of diagnostic or prognosis evaluation kits for renal clear cell carcinoma. Background technique [0002] Renal cell carcinoma (Renal Cell Carcinoma, RCC) is a malignant tumor originating from renal epithelial cells, referred to as renal cancer for short, accounting for about 2%-3% of adult malignant tumors. Kidney cancer accounted for 2.2% of new tumors worldwide in 2018, with a mortality rate of approximately 1.8%. Among urinary system tumors, the incidence of kidney cancer is second only to bladder cancer. my country is the country with the highest incidence of kidney cancer in the world, and the incidence rate of men is about twice that of women. Clear Cell Renal Cell Carcinoma (ccRCC) is the most common pathological type of renal cancer, accounting for about 80%-90%. [0003] Currently, there is no specific tool for early scr...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/577G01N33/58
CPCG01N33/57438G01N33/57484G01N33/577G01N33/581G01N2333/4703
Inventor 崔心刚王超姜宁王宇宁洪天宇彭广王鸿儒曹建伟叶剑青干思舜储传敏
Owner SHANGHAI CITY PUDONG NEW AREA GONGLI HOSPITAL
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