Leveling inspection and calibration device for mouse stereotactic skull

Abstract

The invention discloses a mouse stereotactic skull leveling inspection and calibration device, and belongs to the technical field of medical instruments. The device comprises a skull plate, an anterior fontanel leveling column, a posterior fontanelle leveling column, a left leveling column and a right leveling column, the skull plate is a transparent skull plate, a skull seam is formed in the skull plate, an anterior fontanel hole is formed in the front side, a posterior fontanelle hole sliding groove is formed in the rear side, a left leveling hole sliding groove is formed in the left side, and a right leveling hole sliding groove is formed in the right side; the anterior fontanel leveling column is connected with the anterior fontanel hole, and a front sleeve and a rear sleeve are arranged on the anterior fontanel leveling column; the posterior fontanelle leveling column is connected with the posterior fontanelle hole sliding groove, and a branch pipe is arranged on the posterior fontanelle leveling column. By means of the skull plate, the anterior fontanel leveling column, the posterior fontanelle leveling column, the left leveling column and the right leveling column, the positions of an anterior fontanel and a posterior fontanelle of a mouse skull can be rapidly and accurately determined, the skull horizontal condition can be rapidly and accurately judged, the positioningaccuracy is improved, the positioning step is effectively compressed, time is shortened, operation is easy and convenient, and operation is easy.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a rodent stereotaxic skull leveling inspection and calibration device. Background technique [0002] In neuroscience research, regulating and observing the functions of specific brain regions often requires the use of stereotaxic techniques to accurately inject drugs, viruses, etc. into these brain regions. To achieve precise injection of a specific brain region, it is necessary to determine the three-dimensional coordinates of the nuclei in advance: take the bregma of the skull (the intersection point formed by the sagittal suture and the coronal suture) as the origin of the coordinates, and determine the distance of the brain region by the distance in the XYZ three directions from the origin. position, and then inject the electrodes into the brain according to the XYZ coordinates, and inject the drugs or viruses needed for the experiment. [0003] A key technology to e...

AI Technical Summary

Problems solved by technology

However, due to individual differences in the skulls of rodents, there are variations in the bregma, which leads to deviations in the injection position and experimental errors; on the other hand, this leveling step takes at least half of the stereotaxic time, and the operation is complicated, which seriously reduces the experimental efficiency; Experimenters, especially beginners, often need to practice for a long time at this step, and the largest error in positioning is also generated at this step. Due to the large number of steps and differences in operation, it is more difficult to trace the source of errors

Images