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Truncated protein of nfe2l1 and its application

A protein and nucleic acid molecular technology, applied in the field of biomedicine, can solve the problem of non-transcription factor function of NFE2L1 that has not been seen

Active Publication Date: 2021-07-23
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing NFE2L1 protein modification and cleavage regulation theory, multi-subtype model and other molecular theoretical models are all based on its antioxidant transcription factor function, and there is no research on the possible non-transcription factor function of NFE2L1 in the available range

Method used

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  • Truncated protein of nfe2l1 and its application
  • Truncated protein of nfe2l1 and its application
  • Truncated protein of nfe2l1 and its application

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Embodiment Construction

[0018] Embodiments of the present invention will be described in detail below in conjunction with examples, but the following examples are only used to illustrate the present invention, and should not limit the scope of the present invention. Unless otherwise specified, the reagents, biological materials, culture media and solutions used below are commonly used in the art, publicly available or commercially available.

[0019] Inventors on NFE2L1 1-620 A brief description of the discovery process of its function and anti-tumor effect is as follows:

[0020] 1. NFE2L1 1-620 Expression plasmid construction and expression verification:

[0021] a) Use a total RNA extraction kit to extract total RNA from a human cell line, and use a reverse transcription kit to reverse transcribe the total RNA into cDNA as a template for a PCR reaction.

[0022] b) Use the NFE2L1 gene amplification primers (NFE2L1-F: 5'-CTCGAGGAATTCATGCTTTCTCTGAAGAAATAC-3'; NFE2L1-R: 5'-GGCCGCTCTAGATCACTTTCTCCG...

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Abstract

The present invention relates to the original discovery and application of the truncated protein of the transcription factor NFE2L1. The truncated protein sequence found in the present invention is shown in SEQ ID NO.1, which has the effect of inhibiting tumor growth.

Description

Technical field: [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a truncated protein of NFE2L1 independent of its transcription factor activity and its application in tumors and other related diseases. Background technique: [0002] The redox homeostasis composed of oxidative stress and antioxidant system is a necessary prerequisite for maintaining normal physiological homeostasis. The long-term oxidative damage caused by redox homeostasis imbalance is the cause of many chronic systemic diseases (such as chronic inflammation, diabetes, obesity, neurodegenerative diseases, aging, tumors, etc.). The cap'n'collarbasic-leucine zipper (CNC-bZIP) family of transcription factors is the most powerful transcription factor known to regulate redox homeostasis in eukaryotes group; among the 6 members of mammalian CNC-bZIP transcription factors, nuclear factor erythrocyte factor 2-like factor 1 (NFE2L1 / Nrf1) is the member with the most pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C12N15/12C12N15/85C12N5/10A61K38/17A61P35/00
CPCA61K38/00A61P35/00C07K14/47
Inventor 邱露高艳锋祁元明
Owner ZHENGZHOU UNIV