Flos chinensis extract, its preparation method and use
A technology of dense Mongolian flowers and extracts, applied in the field of medicine, can solve problems such as unknown, unresearched, complex chemical components, etc., and achieve the effects of improving retinal degenerative diseases, inhibiting cell death, and preventing and treating thickness.
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preparation Embodiment 1
[0094] Preparation Example 1: Water extraction and alcohol extraction to extract the active ingredients in the flower
[0095] Weigh 100 g of Chinese herbal medicine pieces of M. japonica, add 1000 ml of 95% ethanol, infiltrate for 0.5 h, heat under reflux at 80 °C for 1 h, and collect the filtrate by filtration; add 1000 ml of 95% ethanol to the filter residue again, extract under reflux at 80 °C for 1 h, filter and combine twice The filtrate was concentrated under reduced pressure using a rotary evaporator to obtain a 95% concentrated solution of the floristry florets extract, which was freeze-dried (19.6 g), number MMH-95.
[0096] After 95% ethanol extraction, 1000 ml of 50% ethanol was added to the filter residue, heated and refluxed at 100 °C for 1 h, and the filtrate was collected by filtration; 1000 ml of 50% ethanol was added to the filter residue again, heated and refluxed at 100 °C for 1 h, filtered and combined two filtrates, and the filtrate was reduced by rotary e...
preparation Embodiment 2
[0100] Preparation Example 2: Water Extraction-Solvent Extraction Method Separation of Active Components in Bulgari
[0101] Weigh 100 g of Chinese herbal medicine pieces of Mimonella japonica, add 1000 ml of distilled water, infiltrate for 0.5 h and then heat and reflux at 100 °C for 1 h, filter and collect the filtrate; add 1000 ml of distilled water to the filter residue again, heat and reflux for 1 h at 100 °C, filter and combine the filtrates twice, and use rotary Evaporator was concentrated under reduced pressure to obtain 200ml of Mimeng flower water extract concentrate, 200ml of ethyl acetate solvent was added for extraction, and after standing to separate layers, the upper layer of ethyl acetate solution was collected. Press-dried to obtain ethyl acetate extraction sample, number MMH-H 2 O-EA; add 200ml of n-butanol solvent to the remaining water layer for extraction, let stand until the layers are separated, collect the upper n-butanol solution, extract 5 times by th...
preparation Embodiment 3
[0102] Preparation Example 3: Purification and Identification of Compounds I to V
[0103] Weigh the MMH-C-H obtained in Preparation Example 2 2 5 g of sample O was dissolved by adding an appropriate amount of distilled water to heat and ultrasonically dissolved, and macroporous resin (D101) was used for column chromatography separation (500 × 60 mm), firstly washed with 1000 ml of distilled water, and then washed with 1000 ml of 20% ethanol and collected the eluate. The deliquoring was analyzed by HPLC to contain main characteristic peaks, and the purity was above 70%. The samples were concentrated and combined to obtain 20% ethanol elution samples. The sample was purified again with ODS chromatography packing, eluted with distilled water and 10% methanol in sequence, concentrated and combined with 10% methanol eluent, and analyzed by HPLC, the purity was 94.14%. Concentrate and dry under reduced pressure to obtain 86.2 mg of brown-yellow solid powder, No. Compound I, which ...
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