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Preparation method of immobilized microbial agent from modified wheat bran material

A technology of immobilizing microorganisms and wheat bran, which is applied in the direction of fixing on/in organic carriers, can solve the problems of poor adsorption, non-renewable, difficult mining, etc., and achieve increased specific surface area, reduced economic costs, and rational utilization resource effect

Active Publication Date: 2020-09-15
ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently commonly used carriers include inorganic carriers (zeolite, kaolin, activated carbon) and organic carriers (corn flour, soybean meal), etc., but these materials have poor adsorption, easy to cause secondary pollution, difficult to mine, non-renewable, and high cost. It is difficult to be used in large quantities in actual waste disposal, so we need a carrier with strong adsorption, high stability, low price and environmental friendliness

Method used

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  • Preparation method of immobilized microbial agent from modified wheat bran material
  • Preparation method of immobilized microbial agent from modified wheat bran material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of a modified wheat bran material immobilized microbial inoculum includes the following steps:

[0020] 1. Bacterial liquid preparation: activate the strains stored in the low-temperature (-40°C) refrigerator, inoculate it into the configured 100mL LB liquid medium, and place it in a constant temperature shaker to ferment for 1d, for later use.

[0021] 2. Carrier preparation:

[0022] (1) Hydrolysis of starch in wheat bran: crush the wheat bran through a 50-mesh sieve, weigh 50g into a triangular flask, and add 500mL NaH 2 PO 4 -NaOH buffer solution, put it in a constant temperature incubator for 50min, then add 1.5g alpha amylase (the mass ratio of wheat bran to alpha amylase is 10:0.3), adjust the pH to 6, put it in a 60℃ environment for hydrolysis After 5h, rinse with distilled water and dry to constant weight. The enzymolysis rate can reach 95%-99%.

[0023] (2) Hydrolysis of protein in wheat bran: Weigh 30g of (1) the starch-removed bran in an Erlenm...

Embodiment 2

[0030] A preparation method of a modified wheat bran material immobilized microbial inoculum includes the following steps:

[0031] 1. Bacterial liquid preparation: activate the strains stored in a low-temperature (-20°C) refrigerator, inoculate it into the configured 100mL LB liquid medium, and place it in a constant temperature shaker to ferment for 2 days, and then set aside.

[0032] 2. Carrier preparation:

[0033] (1) Hydrolysis of starch in wheat bran: crush the wheat bran through a 50-mesh sieve, weigh 100g in a triangular flask, and add 1L NaH 2 PO 4 -NaOH buffer solution, put it in a constant temperature incubator to preheat for 30 minutes, then add 2g of alpha amylase (the mass ratio of wheat bran to alpha amylase is 10:0.2), adjust the pH to 6.5, and hydrolyze it at 65°C for 3 hours Then, rinse with distilled water and dry to constant weight. The enzymatic hydrolysis rate can reach more than 95%.

[0034] (2) Hydrolysis of the protein in wheat bran: Weigh 60g of (1) the s...

Embodiment 3

[0037] A preparation method of a modified wheat bran material immobilized microbial inoculum includes the following steps:

[0038] 1. Bacterial liquid preparation: activate the strains stored in a low-temperature (-80°C) refrigerator, inoculate it into a prepared 100mL LB liquid medium, and place it in a constant temperature shaker to ferment for 1.5 days for use.

[0039] 2. Carrier preparation:

[0040] (1) Hydrolysis of starch in wheat bran: crush the wheat bran through a 50-mesh sieve, weigh 100g in a triangular flask, and add 1L NaH 2 PO 4 -NaOH buffer solution, put it in a constant temperature incubator to preheat for 40 minutes, then add 2.5g alpha amylase (the mass ratio of wheat bran to alpha amylase is 10:0.25), adjust the pH to 6.3, and hydrolyze at 62°C After 4h, rinse with distilled water and dry to constant weight. The enzymatic hydrolysis rate can reach more than 95%.

[0041] (2) Hydrolysis of the protein in wheat bran: Weigh 60g of (1) the starch-removed bran in an ...

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Abstract

The invention discloses a preparation method of an immobilized microbial agent from a modified wheat bran material. The preparation method comprises the following steps of S1, preparing a bacterial solution for later use; S2, carrier preparation, specifically, gradually carrying out amylohydrolysis and proteolytic modification on the wheat bran to prepare a carrier for later use, wherein the hydrolysis sequence is irrespective; and S3, microbial agent preparation, specifically, uniformly mixing the bacterial solution obtained in the step S1 and the carrier obtained in the step S2 according toa ratio of 1mL: (0.5-1) g, and drying to constant weight. Common wheat bran is modified and used as the carrier of the microbial agent, and the immobilization efficiency is high; due to the fact thatthe specific surface area of the modified wheat bran is greatly increased, a rich micropore growth environment is created for microorganisms, the adsorption amount and the containing amount of the microorganisms are increased, the modified wheat bran can be used as a novel carrier, and the viable count in the microbial agent prepared by adopting the carrier far exceeds the executive standard.

Description

Technical field [0001] The present invention relates to the technical field of microbial fermentation, in particular to a preparation method of a modified wheat bran material immobilized microbial agent. Background technique [0002] With the rapid development of my country’s economy and the continuous improvement of people’s living standards, the scale of my country’s planting, breeding, and industry is also growing, and the resulting livestock and poultry manure, garden waste, sludge and kitchen waste, etc. The output of organic solid waste is increasing day by day. Traditional incineration and landfilling are not only easy to cause secondary pollution, but also a waste of resources. In recent years, the technology of using biological method to treat garbage has become more mature. Compared with traditional methods, biological method not only treats garbage thoroughly, has no secondary pollution, and is safe, but also reduces transportation and labor costs. The processed product...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02
CPCC12N11/02
Inventor 丁国春吴娴
Owner ZHONGNONGXINKE SUZHOU ORGANIC RECYCLING RES INST CO LTD
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