Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Immunofluorescence kit for E-Cadherin expression of peripheral blood circulating tumor cells of patient with renal cell carcinoma, and detection method

A technology for peripheral blood circulation and renal cell carcinoma, applied in the field of molecular biology, can solve the problems of inability to evaluate patients, inability to obtain tissue samples in real time or repeatedly, and inability to obtain tissue samples, so as to reduce the loss of cells, avoid edge effects, The effect of avoiding distractions

Inactive Publication Date: 2020-09-18
山东凯歌智能机器有限公司
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the inability of patients with advanced or recurrent renal cell carcinoma to obtain tissue samples through real-time or repeated punctures, and to evaluate the status of E-Cadherin in patients, the present invention provides a non-diagnostic method for E-Cadherin gene mutation in peripheral blood circulating tumor cells of patients with renal cell carcinoma. Objective detection method: use a membrane filtration device to separate and obtain CTCs in the peripheral blood of patients with advanced or recurrent non-renal cell carcinoma whose tissue samples cannot be obtained, and further use immunofluorescence technology to detect the expression of E-Cadherin in CTCs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunofluorescence kit for E-Cadherin expression of peripheral blood circulating tumor cells of patient with renal cell carcinoma, and detection method
  • Immunofluorescence kit for E-Cadherin expression of peripheral blood circulating tumor cells of patient with renal cell carcinoma, and detection method
  • Immunofluorescence kit for E-Cadherin expression of peripheral blood circulating tumor cells of patient with renal cell carcinoma, and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Use a membrane filtration device to separate and obtain CTCs in the peripheral blood of patients with advanced or recurrent renal cell carcinoma whose tissue samples cannot be obtained, and determine whether CTCs exist:

[0049] Collect 5ml of fasting blood from the median cubital vein for 8-12 hours, dilute the peripheral blood with 45ml diluent (ingredients: 1mmol / L EDTA+0.1%BSA+0.1%trehalose+0.2% lauryl polyoxyethylene ether), and then Add 3ml of 4% paraformaldehyde to fix the diluted blood sample for 10 minutes;

[0050] At fixed intervals, assemble the membrane filtration device: as attached figure 1 , figure 2 , image 3 As shown, the filtering device is composed of a filter 3, a filter membrane 7, a blood sample container 2, a waste liquid tank 5, and an iron stand 1;

[0051] Wet the filter 3 with 10ml of PBS, and then add the fixed peripheral blood sample into the blood sample container 2 of the membrane filtration device, so that it is naturally filtere...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of molecular biology, and particularly relates to an immunofluorescence kit for detecting E-Cadherin expression of peripheral blood circulating tumor cellsof a patient with renal cell carcinoma, and a detection method. The immunofluorescence kit comprises a diluents 45 mL, decolorizing liquid 1 mL, a staining solution A 0.5 mL, a staining solution B 1mL, methanol 200 mu l, 2% PFA 200 mu l, 10% goat serum 100 mu l, a primary antibody suspension 100 mu l composed of mouse anti-CK, rat anti-CD45 and rabbit anti-E-Cadherin, a secondary antibody suspension 100 mu l composed of fluorescently-labeled goat anti-mouse, fluorescently-labeled goat anti-rat and fluorescently-labeled goat anti-rabbit, and a DAPI tablet. According to the detection method provided by the invention, the E-Cadherin expression condition of the patient with later-period or relapse renal cell carcinoma can be detected without acquiring a tissue specimen through needle biopsy.The immunofluorescence kit belongs to minimally invasive technology and can realize real-time detection.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to an immunofluorescence kit and a detection method for detecting the expression of E-Cadherin in peripheral blood circulating tumor cells of patients with renal cell carcinoma. Background technique [0002] Renal cell carcinoma (RCC) is one of the most common malignant tumors of the urinary system, its morbidity and mortality account for about 2-3% of systemic malignant tumors, and its incidence has been on the rise in recent decades. Radical surgery is an effective means for the treatment of renal cell carcinoma, but about 20% to 30% of renal cell carcinoma patients have developed distant metastasis at the time of diagnosis. Even after undergoing radical surgery for renal cell carcinoma, 20% to 40% of patients will still experience recurrence and metastasis. Previous studies have suggested that epithelial-mesenchymal transition (EMT) plays an important role ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/574G01N33/569B01D29/05
CPCB01D29/05G01N33/56966G01N33/57438G01N33/57492G01N33/582G01N2333/705
Inventor 李琛琛洪英侯景阳李文涛
Owner 山东凯歌智能机器有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com