Multi-virus immunodetection device and method based on smart phone and micro-fluidic chip

Abstract

The invention discloses a multi-virus immunodetection device and method based on a smart phone and a micro-fluidic chip. The device is used for carrying out multi-virus immunodetection on a blood sample infected with viruses of a human body, a horizontally arranged mobile phone slot is formed in the middle of the upper surface of the shell, a horizontally arranged detection device, a reaction chipand a reagent chip are sequentially arranged above the mobile phone slot, and the reagent chip is driven by a lifting device to vertically move up and down. Reagent bottles with different depths andpenetrable adhesive films at the bottoms are designed, and separation and coupling of the reagent bottles and a sharp tube on the reaction chip is realized, and sample purification, incubation, colordevelopment and the like are integrated on one chip. The square air cavity is controlled to contract and expand by the electromagnetic clamp, so that the requirement of driving liquid to flow in different reaction steps is met, and the existing microplate reader can be replaced by only one smart phone for absorbance detection. The reaction chip is independent of other structures, different antibodies or antigens can be coated in reaction tubes in different reaction chips, and multi-virus detection is realized.

Description

technical field [0001] The invention belongs to the field of biomedical detection, and uses a smart phone and a microfluidic chip to semi-quantitatively detect blood samples infected by human viruses, in particular to a multi-virus immune detection device and method for blood samples infected by human viruses. Background technique [0002] A variety of diseases are acquired by people infected with viruses, which pose a major threat to human survival and health. The detection of these diseases is mainly through blood tests, the most commonly used of which is ELISA, which is based on the specific combination of antigen-antibody, and the degree of disease is judged by detecting the absorbance of the sample after the reaction, which has extremely high sensitivity and specificity. However, this method requires a series of manual and complex operations such as sample addition, incubation, liquid preparation, washing, enzyme addition, incubation, washing, color development, termina...

AI Technical Summary

Problems solved by technology

[0003]With the popularity of smart phones and the integration of smart phone functions, smart phones have great application value in real-time and portable detection, but based on smart phones Devices for biomedical testing have not yet been widely used. It is necessary to design a device that meets the above-mentioned virus detection. Combining with a smart phone is the best choice to minimize costs and improve device portability.

Images